Determination of the antimicrobial and antibiofilm effects and ‘Quorum Sensing’ inhibition potentials of Castanea sativa Mill. extracts
The rapid rise of resistance causes existing antibiotics to become dysfunctional. Therefore, search for new antimicrobial active ingredients has increased in recent years. In this study, flower extracts of Castanea sativa were examined for antimicrobial and anti-quorum sensing aspects. The antimicro...
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doaj-fc4b7a8b8d55405c89273cf6a6bf6f492021-05-02T22:18:48ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092020-03-0148110.15835/nbha48111736Determination of the antimicrobial and antibiofilm effects and ‘Quorum Sensing’ inhibition potentials of Castanea sativa Mill. extractsSaliha EKŞİ0Ülkü Zeynep ÜREYEN ESERTAŞ1Ali Osman KILIÇ2Nebahat EJDER3Barış UZUNOK4Recep Tayyip Erdoğan University, The Faculty of Medicine, Department of Medical Microbiology, 53020 RizeRecep Tayyip Erdoğan University, The Faculty of Medicine, Department of Medical Microbiology, 53020 RizeKaradeniz Technical University, Faculty of Medicine, Department of Medical Microbiology, 61080 TrabzonRecep Tayyip Erdoğan University, The Faculty of Medicine, Department of Medical Microbiology, 53020 Uşak University, Faculty of Medicine, Department of Physiology, 64300 UşakThe rapid rise of resistance causes existing antibiotics to become dysfunctional. Therefore, search for new antimicrobial active ingredients has increased in recent years. In this study, flower extracts of Castanea sativa were examined for antimicrobial and anti-quorum sensing aspects. The antimicrobial properties of methanol, ethyl acetate, ethanol and hexane extracts of C. sativa against some gram-positive and gram-negative bacterial species, as well as yeasts (Candida albicans and Candida parapsilosis) were investigated by the agar well diffusion method. The minimum inhibition concentration (MIC) and minimum bactericidal concentrations (MBC) of C. sativa extracts were also determined. Chromobacterium violaceum ATCC 12472, C. violaceum 35352, C. violaceum VIR07 and C. violaceum CV026 indicator strains were used for determination of the quorum sensing inhibitions, and the Pseudomonas aeruginosa PAO1 strain was used for the swarming tests. Additionally, biofilm inhibition was detected by the spectrophotometric method using the P. aeruginosa PAO1 strain. Methanol, ethanol and ethyl acetate extracts of C. sativa was found to have high antibacterial and antifungal effects, while the methanol extract also had anti-quorum sensing, anti- swarming and biofilm inhibition effects, but no activity was found in the n- hexane extract. To the best of our knowledge this is the first report revealed that methanol extract obtained from C. sativa flowers induced anti-quorum sensing activities mainly inhibited the violacein production, swarming and biofilm formation. The present investigation provided evidence that the C. sativa flower extract maybe a potential source of antimicrobial agents. Therefore, much attention should be paid to C. sativa flower content, which could be used with high efficacy against microorganisms. https://www.notulaebotanicae.ro/index.php/nbha/article/view/11736antimicrobial activity; biofilms; Castanea sativa; quorum sensing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Saliha EKŞİ Ülkü Zeynep ÜREYEN ESERTAŞ Ali Osman KILIÇ Nebahat EJDER Barış UZUNOK |
spellingShingle |
Saliha EKŞİ Ülkü Zeynep ÜREYEN ESERTAŞ Ali Osman KILIÇ Nebahat EJDER Barış UZUNOK Determination of the antimicrobial and antibiofilm effects and ‘Quorum Sensing’ inhibition potentials of Castanea sativa Mill. extracts Notulae Botanicae Horti Agrobotanici Cluj-Napoca antimicrobial activity; biofilms; Castanea sativa; quorum sensing |
author_facet |
Saliha EKŞİ Ülkü Zeynep ÜREYEN ESERTAŞ Ali Osman KILIÇ Nebahat EJDER Barış UZUNOK |
author_sort |
Saliha EKŞİ |
title |
Determination of the antimicrobial and antibiofilm effects and ‘Quorum Sensing’ inhibition potentials of Castanea sativa Mill. extracts |
title_short |
Determination of the antimicrobial and antibiofilm effects and ‘Quorum Sensing’ inhibition potentials of Castanea sativa Mill. extracts |
title_full |
Determination of the antimicrobial and antibiofilm effects and ‘Quorum Sensing’ inhibition potentials of Castanea sativa Mill. extracts |
title_fullStr |
Determination of the antimicrobial and antibiofilm effects and ‘Quorum Sensing’ inhibition potentials of Castanea sativa Mill. extracts |
title_full_unstemmed |
Determination of the antimicrobial and antibiofilm effects and ‘Quorum Sensing’ inhibition potentials of Castanea sativa Mill. extracts |
title_sort |
determination of the antimicrobial and antibiofilm effects and ‘quorum sensing’ inhibition potentials of castanea sativa mill. extracts |
publisher |
AcademicPres |
series |
Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
issn |
0255-965X 1842-4309 |
publishDate |
2020-03-01 |
description |
The rapid rise of resistance causes existing antibiotics to become dysfunctional. Therefore, search for new antimicrobial active ingredients has increased in recent years. In this study, flower extracts of Castanea sativa were examined for antimicrobial and anti-quorum sensing aspects. The antimicrobial properties of methanol, ethyl acetate, ethanol and hexane extracts of C. sativa against some gram-positive and gram-negative bacterial species, as well as yeasts (Candida albicans and Candida parapsilosis) were investigated by the agar well diffusion method. The minimum inhibition concentration (MIC) and minimum bactericidal concentrations (MBC) of C. sativa extracts were also determined. Chromobacterium violaceum ATCC 12472, C. violaceum 35352, C. violaceum VIR07 and C. violaceum CV026 indicator strains were used for determination of the quorum sensing inhibitions, and the Pseudomonas aeruginosa PAO1 strain was used for the swarming tests. Additionally, biofilm inhibition was detected by the spectrophotometric method using the P. aeruginosa PAO1 strain. Methanol, ethanol and ethyl acetate extracts of C. sativa was found to have high antibacterial and antifungal effects, while the methanol extract also had anti-quorum sensing, anti- swarming and biofilm inhibition effects, but no activity was found in the n- hexane extract. To the best of our knowledge this is the first report revealed that methanol extract obtained from C. sativa flowers induced anti-quorum sensing activities mainly inhibited the violacein production, swarming and biofilm formation. The present investigation provided evidence that the C. sativa flower extract maybe a potential source of antimicrobial agents. Therefore, much attention should be paid to C. sativa flower content, which could be used with high efficacy against microorganisms.
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topic |
antimicrobial activity; biofilms; Castanea sativa; quorum sensing |
url |
https://www.notulaebotanicae.ro/index.php/nbha/article/view/11736 |
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