Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line
Aim. Our aim the was to screen the commonly used Chinese herbs in order to detect changes in ERBB2 and ESR1 gene expression using MCF-7 cells. Methods. Using the MCF-7 human breast cancer cell line, cell cytotoxicity and proliferation were evaluated by MTT and trypan blue exclusion assays, respectiv...
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doaj-fc610391581940a180a5ad9466334a012020-11-24T21:27:58ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882014-01-01201410.1155/2014/965486965486Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell LineJen-Hwey Chiu0Chun-Ju Chang1Jing-Chong Wu2Hui-Ju Liu3Che-Sheng Wen4Chung-Hua Hsu5Jiun-Liang Chen6Ling-Ming Tseng7Wei-Shone Chen8Yi-Ming Shyr9Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei 112, TaiwanDepartment of Food Science, National Taiwan Ocean University, Keelung 202, TaiwanCenter for Traditional Chinese Medicine, Kaohsiung Veterans General Hospital, Kaohsiung 81362, TaiwanInstitute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei 112, TaiwanDepartment of Orthopedics, Cheng Hsin General Hospital, Taipei 112, TaiwanInstitute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei 112, TaiwanSchool of Traditional Chinese Medicine, Chang Gung University, Taoyuan 333, TaiwanDivision of General Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei 112, TaiwanDivision of Colorectal Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei 112, TaiwanDivision of General Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei 112, TaiwanAim. Our aim the was to screen the commonly used Chinese herbs in order to detect changes in ERBB2 and ESR1 gene expression using MCF-7 cells. Methods. Using the MCF-7 human breast cancer cell line, cell cytotoxicity and proliferation were evaluated by MTT and trypan blue exclusion assays, respectively. A luciferase reporter assay was established by transient transfecting MCF-7 cells with plasmids containing either the ERBB2 or the ESR1 promoter region linked to the luciferase gene. Chinese herbal extracts were used to treat the cells at 24 h after transfection, followed by measurement of their luciferase activity. The screening results were verified by Western blotting to measure HER2 and ERα protein expression. Results. At concentrations that induced little cytotoxicity, thirteen single herbal extracts and five compound recipes were found to increase either ERBB2 or ESR1 luciferase activity. By Western blotting, Si-Wu-Tang, Kuan-Shin-Yin, and Suan-Tsao-Ren-Tang were found to increase either HER2 or ERα protein expression. In addition, Ligusticum chuanxiong was shown to have a great effect on ERBB2 gene expression and synergistically with estrogen to stimulate MCF-7 cell growth. Conclusion. Our results provide important information that should affect clinical treatment strategies among breast cancer patients who are receiving hormonal or targeted therapies.http://dx.doi.org/10.1155/2014/965486 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jen-Hwey Chiu Chun-Ju Chang Jing-Chong Wu Hui-Ju Liu Che-Sheng Wen Chung-Hua Hsu Jiun-Liang Chen Ling-Ming Tseng Wei-Shone Chen Yi-Ming Shyr |
spellingShingle |
Jen-Hwey Chiu Chun-Ju Chang Jing-Chong Wu Hui-Ju Liu Che-Sheng Wen Chung-Hua Hsu Jiun-Liang Chen Ling-Ming Tseng Wei-Shone Chen Yi-Ming Shyr Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line Evidence-Based Complementary and Alternative Medicine |
author_facet |
Jen-Hwey Chiu Chun-Ju Chang Jing-Chong Wu Hui-Ju Liu Che-Sheng Wen Chung-Hua Hsu Jiun-Liang Chen Ling-Ming Tseng Wei-Shone Chen Yi-Ming Shyr |
author_sort |
Jen-Hwey Chiu |
title |
Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line |
title_short |
Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line |
title_full |
Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line |
title_fullStr |
Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line |
title_full_unstemmed |
Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line |
title_sort |
screening to identify commonly used chinese herbs that affect erbb2 and esr1 gene expression using the human breast cancer mcf-7 cell line |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2014-01-01 |
description |
Aim. Our aim the was to screen the commonly used Chinese herbs in order to detect changes in ERBB2 and ESR1 gene expression using MCF-7 cells. Methods. Using the MCF-7 human breast cancer cell line, cell cytotoxicity and proliferation were evaluated by MTT and trypan blue exclusion assays, respectively. A luciferase reporter assay was established by transient transfecting MCF-7 cells with plasmids containing either the ERBB2 or the ESR1 promoter region linked to the luciferase gene. Chinese herbal extracts were used to treat the cells at 24 h after transfection, followed by measurement of their luciferase activity. The screening results were verified by Western blotting to measure HER2 and ERα protein expression. Results. At concentrations that induced little cytotoxicity, thirteen single herbal extracts and five compound recipes were found to increase either ERBB2 or ESR1 luciferase activity. By Western blotting, Si-Wu-Tang, Kuan-Shin-Yin, and Suan-Tsao-Ren-Tang were found to increase either HER2 or ERα protein expression. In addition, Ligusticum chuanxiong was shown to have a great effect on ERBB2 gene expression and synergistically with estrogen to stimulate MCF-7 cell growth. Conclusion. Our results provide important information that should affect clinical treatment strategies among breast cancer patients who are receiving hormonal or targeted therapies. |
url |
http://dx.doi.org/10.1155/2014/965486 |
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