Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances.
The bacterial strain, EMM-1, was isolated from the rhizosphere of red maize ("Rojo Criollo") and identified as Pseudomonas protegens EMM-1 based on phylogenetic analysis of 16S rDNA, rpoB, rpoD, and gyrB gene sequences. We uncovered genes involved in the production of antimicrobial compoun...
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doaj-fc858ac0a6be4ef2825de686aca3e56d2021-03-04T11:10:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011510e024054510.1371/journal.pone.0240545Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances.Catherine Cesa-LunaAntonino BaezAlberto Aguayo-AcostaRoberto Carlos Llano-VillarrealVíctor Rivelino Juárez-GonzálezPaul GaytánMaría Del Rocío Bustillos-CristalesAmérica Rivera-UrbalejoJesús Muñoz-RojasVerónica Quintero-HernándezThe bacterial strain, EMM-1, was isolated from the rhizosphere of red maize ("Rojo Criollo") and identified as Pseudomonas protegens EMM-1 based on phylogenetic analysis of 16S rDNA, rpoB, rpoD, and gyrB gene sequences. We uncovered genes involved in the production of antimicrobial compounds like 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin, and lectin-like bacteriocins. These antimicrobial compounds are also produced by other fluorescent pseudomonads alike P. protegens. Double-layer agar assay showed that P. protegens EMM-1 inhibited the growth of several multidrug-resistant (MDR) bacteria, especially clinical isolates of the genera Klebsiella and β-hemolytic Streptococcus. This strain also displayed inhibitory effects against diverse fungi, such as Aspergillus, Botrytis, and Fusarium. Besides, a crude extract of inhibitory substances secreted into agar was obtained after the cold-leaching process, and physicochemical characterization was performed. The partially purified inhibitory substances produced by P. protegens EMM-1 inhibited the growth of Streptococcus sp. and Microbacterium sp., but no inhibitory effect was noted for other bacterial or fungal strains. The molecular weight determined after ultrafiltration was between 3 and 10 kDa. The inhibitory activity was thermally stable up to 60°C (but completely lost at 100°C), and the inhibitory activity remained active in a wide pH range (from 3 to 9). After treatment with a protease from Bacillus licheniformis, the inhibitory activity was decreased by 90%, suggesting the presence of proteic natural compounds. All these findings suggested that P. protegens EMM-1 is a potential source of antimicrobials to be used against pathogens for humans and plants.https://doi.org/10.1371/journal.pone.0240545 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Catherine Cesa-Luna Antonino Baez Alberto Aguayo-Acosta Roberto Carlos Llano-Villarreal Víctor Rivelino Juárez-González Paul Gaytán María Del Rocío Bustillos-Cristales América Rivera-Urbalejo Jesús Muñoz-Rojas Verónica Quintero-Hernández |
spellingShingle |
Catherine Cesa-Luna Antonino Baez Alberto Aguayo-Acosta Roberto Carlos Llano-Villarreal Víctor Rivelino Juárez-González Paul Gaytán María Del Rocío Bustillos-Cristales América Rivera-Urbalejo Jesús Muñoz-Rojas Verónica Quintero-Hernández Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances. PLoS ONE |
author_facet |
Catherine Cesa-Luna Antonino Baez Alberto Aguayo-Acosta Roberto Carlos Llano-Villarreal Víctor Rivelino Juárez-González Paul Gaytán María Del Rocío Bustillos-Cristales América Rivera-Urbalejo Jesús Muñoz-Rojas Verónica Quintero-Hernández |
author_sort |
Catherine Cesa-Luna |
title |
Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances. |
title_short |
Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances. |
title_full |
Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances. |
title_fullStr |
Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances. |
title_full_unstemmed |
Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances. |
title_sort |
growth inhibition of pathogenic microorganisms by pseudomonas protegens emm-1 and partial characterization of inhibitory substances. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2020-01-01 |
description |
The bacterial strain, EMM-1, was isolated from the rhizosphere of red maize ("Rojo Criollo") and identified as Pseudomonas protegens EMM-1 based on phylogenetic analysis of 16S rDNA, rpoB, rpoD, and gyrB gene sequences. We uncovered genes involved in the production of antimicrobial compounds like 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin, and lectin-like bacteriocins. These antimicrobial compounds are also produced by other fluorescent pseudomonads alike P. protegens. Double-layer agar assay showed that P. protegens EMM-1 inhibited the growth of several multidrug-resistant (MDR) bacteria, especially clinical isolates of the genera Klebsiella and β-hemolytic Streptococcus. This strain also displayed inhibitory effects against diverse fungi, such as Aspergillus, Botrytis, and Fusarium. Besides, a crude extract of inhibitory substances secreted into agar was obtained after the cold-leaching process, and physicochemical characterization was performed. The partially purified inhibitory substances produced by P. protegens EMM-1 inhibited the growth of Streptococcus sp. and Microbacterium sp., but no inhibitory effect was noted for other bacterial or fungal strains. The molecular weight determined after ultrafiltration was between 3 and 10 kDa. The inhibitory activity was thermally stable up to 60°C (but completely lost at 100°C), and the inhibitory activity remained active in a wide pH range (from 3 to 9). After treatment with a protease from Bacillus licheniformis, the inhibitory activity was decreased by 90%, suggesting the presence of proteic natural compounds. All these findings suggested that P. protegens EMM-1 is a potential source of antimicrobials to be used against pathogens for humans and plants. |
url |
https://doi.org/10.1371/journal.pone.0240545 |
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