Safety of the novel influenza viral vector Brucella abortus vaccine in pregnant heifers

• Main Authors: Kaissar Tabynov, Sholpan Ryskeldinova, Zhailaubay Kydyrbayev, Abylai Sansyzbay
• Format: Article
• Language: English
• Published: Universidade Federal de Santa Maria 2016-01-01
• Series: Ciência Rural
• Subjects: Brucella abortus; vacina; vetor viral; segurança; novilha em gestação; termometria; reatogenicidade; aborto
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100114&lng=en&tlng=en

ABSTRACT: The present study provides the first information about the safety of a new influenza viral vector vaccine expressing the Brucella ribosomal protein L7/L12 or Omp16 containing the adjuvant Montanide Gel01 in pregnant heifers. Immunization of pregnant heifers was conducted via the conjunctival (n=10) or subcutaneous (n=10) route using cross prime and booster vaccination schedules at an interval of 28 days. The vector vaccine was evaluated in comparison with positive control groups vaccinated with B. abortus S19 (n=10) or B. abortus RB51 (n=10) and a negative (PBS+Montanide Gel01; n=10) control group. Clinical studies, thermometry, assessment of local reactogenicity and observation of abortion showed that the vector vaccine via the conjunctival or subcutaneous route was completely safe for pregnant heifers compared to the commercial vaccines B. abortus S19 or B. abortus RB51. The only single adverse event was the formation of infiltration at the site of subcutaneous injection; this reaction was not observed for the conjunctival route.