Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice

Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice

Ribosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes that can inhibit protein translation by depurinating rRNA. Most plant RIPs are synthesized with a leader sequence that sequesters the proteins to a cell compartment away from the host ribosomes. However, several rice RIPs lack th...

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Main Authors: Pieter Wytynck, Jeroen Lambin, Simin Chen, Sinem Demirel Asci, Isabel Verbeke, Jeroen De Zaeytijd, Kondeti Subramanyam, Els J.M. Van Damme
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:International Journal of Molecular Sciences
Subjects:
abiotic stress
localization
<i>Oryza sativa</i>
ribosome-inactivating protein
rice
Online Access:https://www.mdpi.com/1422-0067/22/3/1434
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spelling doaj-fc9bbf48012d42ae9c3933c6133c05672021-02-02T00:00:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01221434143410.3390/ijms22031434Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of RicePieter Wytynck0Jeroen Lambin1Simin Chen2Sinem Demirel Asci3Isabel Verbeke4Jeroen De Zaeytijd5Kondeti Subramanyam6Els J.M. Van Damme7Laboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumLaboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumLaboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumLaboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumLaboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumLaboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumLaboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumLaboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumRibosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes that can inhibit protein translation by depurinating rRNA. Most plant RIPs are synthesized with a leader sequence that sequesters the proteins to a cell compartment away from the host ribosomes. However, several rice RIPs lack these signal peptides suggesting they reside in the cytosol in close proximity to the plant ribosomes. This paper aims to elucidate the physiological function of two nucleocytoplasmic RIPs from rice, in particular, the type 1 RIP referred to as OsRIP1 and a presumed type 3 RIP called nuRIP. Transgenic rice lines overexpressing these RIPs were constructed and studied for developmental effects resulting from this overexpression under greenhouse conditions. In addition, the performance of transgenic seedlings in response to drought, salt, abscisic acid and methyl jasmonate treatment was investigated. Results suggest that both RIPs can affect methyl jasmonate mediated stress responses.https://www.mdpi.com/1422-0067/22/3/1434abiotic stresslocalization<i>Oryza sativa</i>ribosome-inactivating proteinrice
collection DOAJ
language English
format Article
sources DOAJ
author Pieter Wytynck
Jeroen Lambin
Simin Chen
Sinem Demirel Asci
Isabel Verbeke
Jeroen De Zaeytijd
Kondeti Subramanyam
Els J.M. Van Damme
spellingShingle Pieter Wytynck
Jeroen Lambin
Simin Chen
Sinem Demirel Asci
Isabel Verbeke
Jeroen De Zaeytijd
Kondeti Subramanyam
Els J.M. Van Damme
Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice
International Journal of Molecular Sciences
abiotic stress
localization
<i>Oryza sativa</i>
ribosome-inactivating protein
rice
author_facet Pieter Wytynck
Jeroen Lambin
Simin Chen
Sinem Demirel Asci
Isabel Verbeke
Jeroen De Zaeytijd
Kondeti Subramanyam
Els J.M. Van Damme
author_sort Pieter Wytynck
title Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice
title_short Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice
title_full Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice
title_fullStr Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice
title_full_unstemmed Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice
title_sort effect of rip overexpression on abiotic stress tolerance and development of rice
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1661-6596
1422-0067
publishDate 2021-02-01
description Ribosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes that can inhibit protein translation by depurinating rRNA. Most plant RIPs are synthesized with a leader sequence that sequesters the proteins to a cell compartment away from the host ribosomes. However, several rice RIPs lack these signal peptides suggesting they reside in the cytosol in close proximity to the plant ribosomes. This paper aims to elucidate the physiological function of two nucleocytoplasmic RIPs from rice, in particular, the type 1 RIP referred to as OsRIP1 and a presumed type 3 RIP called nuRIP. Transgenic rice lines overexpressing these RIPs were constructed and studied for developmental effects resulting from this overexpression under greenhouse conditions. In addition, the performance of transgenic seedlings in response to drought, salt, abscisic acid and methyl jasmonate treatment was investigated. Results suggest that both RIPs can affect methyl jasmonate mediated stress responses.
topic abiotic stress
localization
<i>Oryza sativa</i>
ribosome-inactivating protein
rice
url https://www.mdpi.com/1422-0067/22/3/1434
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AT jeroenlambin effectofripoverexpressiononabioticstresstoleranceanddevelopmentofrice
AT siminchen effectofripoverexpressiononabioticstresstoleranceanddevelopmentofrice
AT sinemdemirelasci effectofripoverexpressiononabioticstresstoleranceanddevelopmentofrice
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