Effects of quercetin on Aroclor 1254-induced expression of CYP450 and cytokines in pregnant rats
The present study aimed to investigate the protective effect of quercetin on polychlorinated biphenyls (PCB)-induced liver and embryo damage in pregnant Sprague–Dawley rats. Pregnant rats were divided into five groups, and then were orally gavaged daily with peanut oil (vehicle) or a commercial PCB...
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doaj-fca63d2a120c45738dd72aabd5c22f912020-11-25T03:34:58ZengTaylor & Francis GroupJournal of Immunotoxicology1547-691X1547-69012019-01-0116114014810.1080/1547691X.2019.16045851604585Effects of quercetin on Aroclor 1254-induced expression of CYP450 and cytokines in pregnant ratsLina Xu0Xiaojun Guo1Nan Li2Qing Pan3Yu Zhong Ma4Hebei University of EngineeringHebei Agricultural UniversityHebei Agricultural UniversityLangfang Polytechnic InstituteHebei Agricultural UniversityThe present study aimed to investigate the protective effect of quercetin on polychlorinated biphenyls (PCB)-induced liver and embryo damage in pregnant Sprague–Dawley rats. Pregnant rats were divided into five groups, and then were orally gavaged daily with peanut oil (vehicle) or a commercial PCB mixture (Aroclor 1254) — with or without co-treatment with 75, 150, or 300 mg/kg quercetin — on gestation days (GD) 4–7. At GD 9, all rats were euthanized, and their blood, liver, and uterus were collected. Expressions of CYP450 mRNA and protein in liver, cytokines (IFNγ, IL-2, IL-4, and IL-6) and IFNγ/IL-4 ratios in liver and sera, liver morphology, and the status of implanted embryos were analyzed. The results showed Aroclor 1254 treatment alone caused hepatic cord damage (i.e. cell disorganization, swelling, decreased cytoplasm, vacuolization), and that quercetin co-treatment appeared to mitigate this damage. Similarly, levels of CYP1A1 and CYP2B1 mRNA in livers of Aroclor 1254-only-treated rats were significantly higher than those in rats co-treated with quercetin. Hepatic and sera levels of IFNγ, IL-2, IL-6, and IFNγ/IL-4 ratios, and the ratio of delayed-development embryos, all increased in Aroclor 1254-treated rats, but were relatively decreased as a result of quercetin co-treatments. IL-4 levels were decreased by Aroclor 1254 and tended to increase back to normal when quercetin was used. The results indicated that quercetin imparted a protective effect against Aroclor 1254-induced toxicity in pregnant rats, in part, by modulating levels of important pro-inflammatory cytokines and reducing induced CYP1A1 and CYP2B1 expression.http://dx.doi.org/10.1080/1547691X.2019.1604585quercetinaroclor 1254ratlivercyp450cytokinesifnγ/il-4embryo |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lina Xu Xiaojun Guo Nan Li Qing Pan Yu Zhong Ma |
spellingShingle |
Lina Xu Xiaojun Guo Nan Li Qing Pan Yu Zhong Ma Effects of quercetin on Aroclor 1254-induced expression of CYP450 and cytokines in pregnant rats Journal of Immunotoxicology quercetin aroclor 1254 rat liver cyp450 cytokines ifnγ/il-4 embryo |
author_facet |
Lina Xu Xiaojun Guo Nan Li Qing Pan Yu Zhong Ma |
author_sort |
Lina Xu |
title |
Effects of quercetin on Aroclor 1254-induced expression of CYP450 and cytokines in pregnant rats |
title_short |
Effects of quercetin on Aroclor 1254-induced expression of CYP450 and cytokines in pregnant rats |
title_full |
Effects of quercetin on Aroclor 1254-induced expression of CYP450 and cytokines in pregnant rats |
title_fullStr |
Effects of quercetin on Aroclor 1254-induced expression of CYP450 and cytokines in pregnant rats |
title_full_unstemmed |
Effects of quercetin on Aroclor 1254-induced expression of CYP450 and cytokines in pregnant rats |
title_sort |
effects of quercetin on aroclor 1254-induced expression of cyp450 and cytokines in pregnant rats |
publisher |
Taylor & Francis Group |
series |
Journal of Immunotoxicology |
issn |
1547-691X 1547-6901 |
publishDate |
2019-01-01 |
description |
The present study aimed to investigate the protective effect of quercetin on polychlorinated biphenyls (PCB)-induced liver and embryo damage in pregnant Sprague–Dawley rats. Pregnant rats were divided into five groups, and then were orally gavaged daily with peanut oil (vehicle) or a commercial PCB mixture (Aroclor 1254) — with or without co-treatment with 75, 150, or 300 mg/kg quercetin — on gestation days (GD) 4–7. At GD 9, all rats were euthanized, and their blood, liver, and uterus were collected. Expressions of CYP450 mRNA and protein in liver, cytokines (IFNγ, IL-2, IL-4, and IL-6) and IFNγ/IL-4 ratios in liver and sera, liver morphology, and the status of implanted embryos were analyzed. The results showed Aroclor 1254 treatment alone caused hepatic cord damage (i.e. cell disorganization, swelling, decreased cytoplasm, vacuolization), and that quercetin co-treatment appeared to mitigate this damage. Similarly, levels of CYP1A1 and CYP2B1 mRNA in livers of Aroclor 1254-only-treated rats were significantly higher than those in rats co-treated with quercetin. Hepatic and sera levels of IFNγ, IL-2, IL-6, and IFNγ/IL-4 ratios, and the ratio of delayed-development embryos, all increased in Aroclor 1254-treated rats, but were relatively decreased as a result of quercetin co-treatments. IL-4 levels were decreased by Aroclor 1254 and tended to increase back to normal when quercetin was used. The results indicated that quercetin imparted a protective effect against Aroclor 1254-induced toxicity in pregnant rats, in part, by modulating levels of important pro-inflammatory cytokines and reducing induced CYP1A1 and CYP2B1 expression. |
topic |
quercetin aroclor 1254 rat liver cyp450 cytokines ifnγ/il-4 embryo |
url |
http://dx.doi.org/10.1080/1547691X.2019.1604585 |
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