Botrytis cinerea in raspberry in Serbia I: Morphological and molecular characterization
Morphological and molecular characterisation of 130 isolates of Botrytis cinerea, derived from raspberry fruit originating from six commercial fields in a raspberry growing region of Serbia (locations: Požega, Prilike, Arilje, Ivanjica, Šabac and Valjevo) was performed. The results showed that al...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Institute of Pesticides and Environmental Protection
2014-12-01
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| Series: | Pesticidi i Fitomedicina |
| Subjects: | |
| Online Access: | http://www.pesting.org.rs/download.php/documents/29-4/29-4%20237-247.pdf |
| Summary: | Morphological and molecular characterisation of 130 isolates of Botrytis cinerea, derived
from raspberry fruit originating from six commercial fields in a raspberry growing region
of Serbia (locations: Požega, Prilike, Arilje, Ivanjica, Šabac and Valjevo) was performed. The
results showed that all isolates formed white, uniform, aerial mycelia with entire margin on
PDA medium. First morphological differences among the isolates appeared after six days
of incubation. Three-week old isolates were grouped into eight distinct morphological
types – four mycelial and four sclerotial. Mostly, they were of sclerotial type (81.5%) and
the most frequently found was an S3 type, which formed large irregularly placed sclerotia.
This type was dominant in five of six investigated locations and represented 45-65% of the
isolates. The least frequent was the mycelial type M3 (0.7% of the isolates) characterized
by mycelial masses.
The presence of Boty and/or Flipper transposons was detected in isolates originating
from all investigated locations. It was discovered that the B. cinerea population in raspberry
in Serbia, besides the well-described genetically isolated sympatric species transposa
(43.1%) and vacuma (10.8%), contains also another two, boty (44.6%) and flipper (1.5%)
species with only one transposon (either Boty or Flipper) in the genome. In addition, it
was revealed that all isolates from raspberry collected in Serbia, transposa, vacuma, boty
or flipper, are sensitive or weakly resistant to fenhexamid and therefore belong to the B.
cinerea genetical Group II. |
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| ISSN: | 1820-3949 1820-3949 |