Comparative histochemical study of four enzyme activities on some organs of male Schistosoma mansoni and S. haematobium
Abstract Background Enzymes are the biological catalysts which accelerate the various cellular reactions. In general, the enzymes alkaline phosphatase (ALPase), acid phosphatase (ACPase), 5′-nucleotidase (5′-Nu) and glucose-6-phosphatase (G-6-Pase) have important roles in metabolism. In the present...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
SpringerOpen
2018-03-01
|
Series: | Journal of Basic and Applied Zoology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s41936-018-0034-6 |
id |
doaj-fcbf0b2738464a07a9098c9159695e0f |
---|---|
record_format |
Article |
spelling |
doaj-fcbf0b2738464a07a9098c9159695e0f2020-11-24T21:42:21ZengSpringerOpenJournal of Basic and Applied Zoology2090-990X2018-03-0179111010.1186/s41936-018-0034-6Comparative histochemical study of four enzyme activities on some organs of male Schistosoma mansoni and S. haematobiumEman A. El-Shabasy0Enayat S. Reda1Ashraf E. Said2Mai A. Saleh3Department of Zoology, Faculty of Science, Mansoura UniversityDepartment of Zoology, Faculty of Science, Mansoura UniversityDepartment of Zoology, Faculty of Science, Damietta UniversityDepartment of Zoology, Faculty of Science, Mansoura UniversityAbstract Background Enzymes are the biological catalysts which accelerate the various cellular reactions. In general, the enzymes alkaline phosphatase (ALPase), acid phosphatase (ACPase), 5′-nucleotidase (5′-Nu) and glucose-6-phosphatase (G-6-Pase) have important roles in metabolism. In the present study, a comparative account of the localization of activity of four enzymes acid phosphatase, alkaline phosphatase, glucose-6-phosphatase and 5′-nucleotidase was carried out in the organs and tissues of important human digeneans Schistosoma mansoni and Schistosoma haematobium from infected hamster (Cricentus auratus) in Egypt. Material and methods Histochemical methods were used on whole parasites to study the distribution of these enzymes. The following organs and tissues were studied: oral sucker, oesophagus, oesphageal gland, ventral sucker, ventral sucker rim, gynaecophoral canal edge, intestine, tubercles, muscles, tegumental folds, testes and excretory pore. Results Variable observations in the different organs and tissues were recorded. In S. mansoni, glucose-6-phosphatase was detected with the highest activity while alkaline phosphatase showed the lowest activity in most organs and tissues. In S. haematobium, acid phosphatase showed the highest activity and alkaline phosphatase showed the lowest activity in most organs and tissues. Although all enzymes showed clear activity in the oral sucker for both species, only glucose-6-phosphatase gave a positive reaction in the ventral sucker rim and acid phosphatase in the excretory pore of S. haematobium. On the other hand, all enzymes showed no activity in the muscles and excretory pore in S. mansoni. Conclusion ACPase activity was observed in most organs of S. haematobium. Also, G-6-Pase was detected in the ventral sucker rim and 5′-Nu was detected in tubercles. Meanwhile, ACPase and 5′-Nu were observed in the muscle fibers, testes, excretory pore and tegumental folds. On other hand, G-6-Pase is the most active enzyme in most organs of S. mansoni except the muscle fibers.http://link.springer.com/article/10.1186/s41936-018-0034-6Acid phosphataseAlkaline phosphataseGlucose-6-phosphatase and 5′-nucleotidaseSchistosoma mansoniS. haematobiumHistochemical study |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eman A. El-Shabasy Enayat S. Reda Ashraf E. Said Mai A. Saleh |
spellingShingle |
Eman A. El-Shabasy Enayat S. Reda Ashraf E. Said Mai A. Saleh Comparative histochemical study of four enzyme activities on some organs of male Schistosoma mansoni and S. haematobium Journal of Basic and Applied Zoology Acid phosphatase Alkaline phosphatase Glucose-6-phosphatase and 5′-nucleotidase Schistosoma mansoni S. haematobium Histochemical study |
author_facet |
Eman A. El-Shabasy Enayat S. Reda Ashraf E. Said Mai A. Saleh |
author_sort |
Eman A. El-Shabasy |
title |
Comparative histochemical study of four enzyme activities on some organs of male Schistosoma mansoni and S. haematobium |
title_short |
Comparative histochemical study of four enzyme activities on some organs of male Schistosoma mansoni and S. haematobium |
title_full |
Comparative histochemical study of four enzyme activities on some organs of male Schistosoma mansoni and S. haematobium |
title_fullStr |
Comparative histochemical study of four enzyme activities on some organs of male Schistosoma mansoni and S. haematobium |
title_full_unstemmed |
Comparative histochemical study of four enzyme activities on some organs of male Schistosoma mansoni and S. haematobium |
title_sort |
comparative histochemical study of four enzyme activities on some organs of male schistosoma mansoni and s. haematobium |
publisher |
SpringerOpen |
series |
Journal of Basic and Applied Zoology |
issn |
2090-990X |
publishDate |
2018-03-01 |
description |
Abstract Background Enzymes are the biological catalysts which accelerate the various cellular reactions. In general, the enzymes alkaline phosphatase (ALPase), acid phosphatase (ACPase), 5′-nucleotidase (5′-Nu) and glucose-6-phosphatase (G-6-Pase) have important roles in metabolism. In the present study, a comparative account of the localization of activity of four enzymes acid phosphatase, alkaline phosphatase, glucose-6-phosphatase and 5′-nucleotidase was carried out in the organs and tissues of important human digeneans Schistosoma mansoni and Schistosoma haematobium from infected hamster (Cricentus auratus) in Egypt. Material and methods Histochemical methods were used on whole parasites to study the distribution of these enzymes. The following organs and tissues were studied: oral sucker, oesophagus, oesphageal gland, ventral sucker, ventral sucker rim, gynaecophoral canal edge, intestine, tubercles, muscles, tegumental folds, testes and excretory pore. Results Variable observations in the different organs and tissues were recorded. In S. mansoni, glucose-6-phosphatase was detected with the highest activity while alkaline phosphatase showed the lowest activity in most organs and tissues. In S. haematobium, acid phosphatase showed the highest activity and alkaline phosphatase showed the lowest activity in most organs and tissues. Although all enzymes showed clear activity in the oral sucker for both species, only glucose-6-phosphatase gave a positive reaction in the ventral sucker rim and acid phosphatase in the excretory pore of S. haematobium. On the other hand, all enzymes showed no activity in the muscles and excretory pore in S. mansoni. Conclusion ACPase activity was observed in most organs of S. haematobium. Also, G-6-Pase was detected in the ventral sucker rim and 5′-Nu was detected in tubercles. Meanwhile, ACPase and 5′-Nu were observed in the muscle fibers, testes, excretory pore and tegumental folds. On other hand, G-6-Pase is the most active enzyme in most organs of S. mansoni except the muscle fibers. |
topic |
Acid phosphatase Alkaline phosphatase Glucose-6-phosphatase and 5′-nucleotidase Schistosoma mansoni S. haematobium Histochemical study |
url |
http://link.springer.com/article/10.1186/s41936-018-0034-6 |
work_keys_str_mv |
AT emanaelshabasy comparativehistochemicalstudyoffourenzymeactivitiesonsomeorgansofmaleschistosomamansoniandshaematobium AT enayatsreda comparativehistochemicalstudyoffourenzymeactivitiesonsomeorgansofmaleschistosomamansoniandshaematobium AT ashrafesaid comparativehistochemicalstudyoffourenzymeactivitiesonsomeorgansofmaleschistosomamansoniandshaematobium AT maiasaleh comparativehistochemicalstudyoffourenzymeactivitiesonsomeorgansofmaleschistosomamansoniandshaematobium |
_version_ |
1725917433927041024 |