Lactate as a Potential Biomarker of Sepsis in a Rat Cecal Ligation and Puncture Model
We attempted to investigate whether blood lactate is a useful biomarker for sepsis in a rat cecal ligation and puncture (CLP) model. Male Sprague-Dawley rats underwent approximately 75% cecum ligation and two punctures to induce high-grade sepsis. A lactate of 1.64 mmol/L (Youden score of 0.722) was...
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Series: | Mediators of Inflammation |
Online Access: | http://dx.doi.org/10.1155/2018/8352727 |
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doaj-fcc5c62e36744f55ac318e8292b4cadc2020-11-24T23:01:13ZengHindawi LimitedMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/83527278352727Lactate as a Potential Biomarker of Sepsis in a Rat Cecal Ligation and Puncture ModelXiaozhu Zhai0Zhengfei Yang1Guanghui Zheng2Tao Yu3Peng Wang4Xuefen Liu5Qin Ling6Longyuan Jiang7Wanchun Tang8Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaSun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaSun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaSun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaSun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaSun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaSun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaSun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaSun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaWe attempted to investigate whether blood lactate is a useful biomarker for sepsis in a rat cecal ligation and puncture (CLP) model. Male Sprague-Dawley rats underwent approximately 75% cecum ligation and two punctures to induce high-grade sepsis. A lactate of 1.64 mmol/L (Youden score of 0.722) was selected as the best cutoff value to predict the onset of sepsis after CLP exposure; 46 of 50 rats who survived 24 hours after the CLP were divided into the L group (lactate < 1.64 mmol/L) and M group (lactate ≥ 1.64 mmol/L). In the M group, the animals had significantly higher murine sepsis scores and none survived 5 days post-CLP, and the rate of validated septic animals, serum procalcitonin, high mobility group box 1, blood urea nitrogen, alanine transaminase, cardiac troponin I, and the wet-to-dry weight ratio were significantly higher compared to the L group. Worsen PaO2/FiO2, microcirculations, and mean arterial pressure were observed in the M group. More severe damage in major organs was confirmed by histopathological scores in the M group compared with the L group. In conclusion, lactate ≥ 1.64 mmol/L might serve as a potential biomarker to identify the onset of sepsis in a rat CLP model.http://dx.doi.org/10.1155/2018/8352727 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaozhu Zhai Zhengfei Yang Guanghui Zheng Tao Yu Peng Wang Xuefen Liu Qin Ling Longyuan Jiang Wanchun Tang |
spellingShingle |
Xiaozhu Zhai Zhengfei Yang Guanghui Zheng Tao Yu Peng Wang Xuefen Liu Qin Ling Longyuan Jiang Wanchun Tang Lactate as a Potential Biomarker of Sepsis in a Rat Cecal Ligation and Puncture Model Mediators of Inflammation |
author_facet |
Xiaozhu Zhai Zhengfei Yang Guanghui Zheng Tao Yu Peng Wang Xuefen Liu Qin Ling Longyuan Jiang Wanchun Tang |
author_sort |
Xiaozhu Zhai |
title |
Lactate as a Potential Biomarker of Sepsis in a Rat Cecal Ligation and Puncture Model |
title_short |
Lactate as a Potential Biomarker of Sepsis in a Rat Cecal Ligation and Puncture Model |
title_full |
Lactate as a Potential Biomarker of Sepsis in a Rat Cecal Ligation and Puncture Model |
title_fullStr |
Lactate as a Potential Biomarker of Sepsis in a Rat Cecal Ligation and Puncture Model |
title_full_unstemmed |
Lactate as a Potential Biomarker of Sepsis in a Rat Cecal Ligation and Puncture Model |
title_sort |
lactate as a potential biomarker of sepsis in a rat cecal ligation and puncture model |
publisher |
Hindawi Limited |
series |
Mediators of Inflammation |
issn |
0962-9351 1466-1861 |
publishDate |
2018-01-01 |
description |
We attempted to investigate whether blood lactate is a useful biomarker for sepsis in a rat cecal ligation and puncture (CLP) model. Male Sprague-Dawley rats underwent approximately 75% cecum ligation and two punctures to induce high-grade sepsis. A lactate of 1.64 mmol/L (Youden score of 0.722) was selected as the best cutoff value to predict the onset of sepsis after CLP exposure; 46 of 50 rats who survived 24 hours after the CLP were divided into the L group (lactate < 1.64 mmol/L) and M group (lactate ≥ 1.64 mmol/L). In the M group, the animals had significantly higher murine sepsis scores and none survived 5 days post-CLP, and the rate of validated septic animals, serum procalcitonin, high mobility group box 1, blood urea nitrogen, alanine transaminase, cardiac troponin I, and the wet-to-dry weight ratio were significantly higher compared to the L group. Worsen PaO2/FiO2, microcirculations, and mean arterial pressure were observed in the M group. More severe damage in major organs was confirmed by histopathological scores in the M group compared with the L group. In conclusion, lactate ≥ 1.64 mmol/L might serve as a potential biomarker to identify the onset of sepsis in a rat CLP model. |
url |
http://dx.doi.org/10.1155/2018/8352727 |
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