Isolation and Identification of Nocardia spp. Using Phenotypic Methods from Soil Samples of North Khorasan Province
<p><strong><em>Introduction</em></strong><strong>: </strong><em>N</em><em>o</em><em>c</em><em>a</em><em>rdia </em>is saprophytic soil bacterium of the actinomycetes families. <em>Nocardia <...
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doaj-fcc7984573b343c9865cb4d7e47321a92020-11-24T22:15:04ZengTehran University of Medical SciencesJournal of Medical Bacteriology2251-86492322-25812015-11-0141-281452Isolation and Identification of Nocardia spp. Using Phenotypic Methods from Soil Samples of North Khorasan ProvinceFatemeh Andalibi0Mehdi Fatahi-Bafghi1Parvin Heidarieh2Masoumeh Rasouli-Nasab3Shadi Habibnia4MohammadReza Pourmand5SeyyedSaeed Eshraghi6Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, - Student Research Center, Tehran, IR IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR IranDepartment of Bacteriology and Virology, School of Medicine, Alborz University of Medical Sciences, Karaj, IR IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran<p><strong><em>Introduction</em></strong><strong>: </strong><em>N</em><em>o</em><em>c</em><em>a</em><em>rdia </em>is saprophytic soil bacterium of the actinomycetes families. <em>Nocardia </em>has high diversity of species and due to species diversity of <em>Nocardia</em>, phenotypic tests are essential for early identification of <em>Nocardia </em>species.</p><p><strong><em>M</em></strong><strong><em>e</em></strong><strong><em>thods</em></strong><strong>: </strong>155 soil samples from different regions of North Khorasan province in Iran were collected, including: urban and rural areas. Isolation of <em>Nocardia </em>was performed by paraffin baiting Technique. Identification of <em>Nocardia </em>species was performed by phenotypic methods that are including: hydrolysis of the amino acids, acid production of carbohydrates and, growth at 35° C and 45°C.</p><p><strong><em>Results</em></strong><strong>: </strong>11 <em>Nocardia </em>species (7%) were identified . These bacteria were related to the gardens, the sands soils, and soil of town square. No growth was observed at 45°C. As the result, our isolates were identified as <em>Nocardia asteroides </em>complex.</p><p><strong><em>Conclusion</em></strong><strong>: </strong>Isolation and identification of <em>Nocardia </em>spp. from soil of different regions in North Khorasan province in Iran can help to enhance our understanding of epidemiological and ecological of the pathogenic <em>Nocardia </em>species.</p>http://jmb.tums.ac.ir/index.php/jmb/article/view/59Nocardia, soil Paraffin baiting, Phenotypic tests, Khorasan |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fatemeh Andalibi Mehdi Fatahi-Bafghi Parvin Heidarieh Masoumeh Rasouli-Nasab Shadi Habibnia MohammadReza Pourmand SeyyedSaeed Eshraghi |
spellingShingle |
Fatemeh Andalibi Mehdi Fatahi-Bafghi Parvin Heidarieh Masoumeh Rasouli-Nasab Shadi Habibnia MohammadReza Pourmand SeyyedSaeed Eshraghi Isolation and Identification of Nocardia spp. Using Phenotypic Methods from Soil Samples of North Khorasan Province Journal of Medical Bacteriology Nocardia, soil Paraffin baiting, Phenotypic tests, Khorasan |
author_facet |
Fatemeh Andalibi Mehdi Fatahi-Bafghi Parvin Heidarieh Masoumeh Rasouli-Nasab Shadi Habibnia MohammadReza Pourmand SeyyedSaeed Eshraghi |
author_sort |
Fatemeh Andalibi |
title |
Isolation and Identification of Nocardia spp. Using Phenotypic Methods from Soil Samples of North Khorasan Province |
title_short |
Isolation and Identification of Nocardia spp. Using Phenotypic Methods from Soil Samples of North Khorasan Province |
title_full |
Isolation and Identification of Nocardia spp. Using Phenotypic Methods from Soil Samples of North Khorasan Province |
title_fullStr |
Isolation and Identification of Nocardia spp. Using Phenotypic Methods from Soil Samples of North Khorasan Province |
title_full_unstemmed |
Isolation and Identification of Nocardia spp. Using Phenotypic Methods from Soil Samples of North Khorasan Province |
title_sort |
isolation and identification of nocardia spp. using phenotypic methods from soil samples of north khorasan province |
publisher |
Tehran University of Medical Sciences |
series |
Journal of Medical Bacteriology |
issn |
2251-8649 2322-2581 |
publishDate |
2015-11-01 |
description |
<p><strong><em>Introduction</em></strong><strong>: </strong><em>N</em><em>o</em><em>c</em><em>a</em><em>rdia </em>is saprophytic soil bacterium of the actinomycetes families. <em>Nocardia </em>has high diversity of species and due to species diversity of <em>Nocardia</em>, phenotypic tests are essential for early identification of <em>Nocardia </em>species.</p><p><strong><em>M</em></strong><strong><em>e</em></strong><strong><em>thods</em></strong><strong>: </strong>155 soil samples from different regions of North Khorasan province in Iran were collected, including: urban and rural areas. Isolation of <em>Nocardia </em>was performed by paraffin baiting Technique. Identification of <em>Nocardia </em>species was performed by phenotypic methods that are including: hydrolysis of the amino acids, acid production of carbohydrates and, growth at 35° C and 45°C.</p><p><strong><em>Results</em></strong><strong>: </strong>11 <em>Nocardia </em>species (7%) were identified . These bacteria were related to the gardens, the sands soils, and soil of town square. No growth was observed at 45°C. As the result, our isolates were identified as <em>Nocardia asteroides </em>complex.</p><p><strong><em>Conclusion</em></strong><strong>: </strong>Isolation and identification of <em>Nocardia </em>spp. from soil of different regions in North Khorasan province in Iran can help to enhance our understanding of epidemiological and ecological of the pathogenic <em>Nocardia </em>species.</p> |
topic |
Nocardia, soil Paraffin baiting, Phenotypic tests, Khorasan |
url |
http://jmb.tums.ac.ir/index.php/jmb/article/view/59 |
work_keys_str_mv |
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