SNAREs Controlling Vesicular Release of BDNF and Development of Callosal Axons
At presynaptic active zones, exocytosis of neurotransmitter vesicles (SVs) is driven by SNARE complexes that recruit Syb2 and SNAP25. However, it remains unknown which SNAREs promote the secretion of neuronal proteins, including those essential for circuit development and experience-dependent plasti...
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doaj-fcd323bfb0454da59e71731c6fece7f92020-11-24T22:14:29ZengElsevierCell Reports2211-12472015-05-011171054106610.1016/j.celrep.2015.04.032SNAREs Controlling Vesicular Release of BDNF and Development of Callosal AxonsMasafumi Shimojo0Julien Courchet1Simon Pieraut2Nina Torabi-Rander3Richard Sando III4Franck Polleux5Anton Maximov6Department of Molecular and Cellular Neuroscience, The Scripps Research Institute (TSRI), La Jolla, CA 92037, USADepartment of Molecular and Cellular Neuroscience, The Scripps Research Institute (TSRI), La Jolla, CA 92037, USADepartment of Molecular and Cellular Neuroscience, The Scripps Research Institute (TSRI), La Jolla, CA 92037, USADepartment of Molecular and Cellular Neuroscience, The Scripps Research Institute (TSRI), La Jolla, CA 92037, USADepartment of Molecular and Cellular Neuroscience, The Scripps Research Institute (TSRI), La Jolla, CA 92037, USADepartment of Molecular and Cellular Neuroscience, The Scripps Research Institute (TSRI), La Jolla, CA 92037, USADepartment of Molecular and Cellular Neuroscience, The Scripps Research Institute (TSRI), La Jolla, CA 92037, USAAt presynaptic active zones, exocytosis of neurotransmitter vesicles (SVs) is driven by SNARE complexes that recruit Syb2 and SNAP25. However, it remains unknown which SNAREs promote the secretion of neuronal proteins, including those essential for circuit development and experience-dependent plasticity. Here we demonstrate that Syb2 and SNAP25 mediate the vesicular release of BDNF in axons and dendrites of cortical neurons, suggesting these SNAREs act in multiple spatially segregated secretory pathways. Remarkably, axonal secretion of BDNF is also strongly regulated by SNAP47, which interacts with SNAP25 but appears to be dispensable for exocytosis of SVs. Cell-autonomous ablation of SNAP47 disrupts the layer-specific branching of callosal axons of projection cortical neurons in vivo, and this phenotype is recapitulated by ablation of BDNF or its receptor, TrkB. Our results provide insights into the molecular mechanisms of protein secretion, and they define the functions of SNAREs in BDNF signaling and regulation of neuronal connectivity.http://www.sciencedirect.com/science/article/pii/S2211124715004337 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Masafumi Shimojo Julien Courchet Simon Pieraut Nina Torabi-Rander Richard Sando III Franck Polleux Anton Maximov |
spellingShingle |
Masafumi Shimojo Julien Courchet Simon Pieraut Nina Torabi-Rander Richard Sando III Franck Polleux Anton Maximov SNAREs Controlling Vesicular Release of BDNF and Development of Callosal Axons Cell Reports |
author_facet |
Masafumi Shimojo Julien Courchet Simon Pieraut Nina Torabi-Rander Richard Sando III Franck Polleux Anton Maximov |
author_sort |
Masafumi Shimojo |
title |
SNAREs Controlling Vesicular Release of BDNF and Development of Callosal Axons |
title_short |
SNAREs Controlling Vesicular Release of BDNF and Development of Callosal Axons |
title_full |
SNAREs Controlling Vesicular Release of BDNF and Development of Callosal Axons |
title_fullStr |
SNAREs Controlling Vesicular Release of BDNF and Development of Callosal Axons |
title_full_unstemmed |
SNAREs Controlling Vesicular Release of BDNF and Development of Callosal Axons |
title_sort |
snares controlling vesicular release of bdnf and development of callosal axons |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2015-05-01 |
description |
At presynaptic active zones, exocytosis of neurotransmitter vesicles (SVs) is driven by SNARE complexes that recruit Syb2 and SNAP25. However, it remains unknown which SNAREs promote the secretion of neuronal proteins, including those essential for circuit development and experience-dependent plasticity. Here we demonstrate that Syb2 and SNAP25 mediate the vesicular release of BDNF in axons and dendrites of cortical neurons, suggesting these SNAREs act in multiple spatially segregated secretory pathways. Remarkably, axonal secretion of BDNF is also strongly regulated by SNAP47, which interacts with SNAP25 but appears to be dispensable for exocytosis of SVs. Cell-autonomous ablation of SNAP47 disrupts the layer-specific branching of callosal axons of projection cortical neurons in vivo, and this phenotype is recapitulated by ablation of BDNF or its receptor, TrkB. Our results provide insights into the molecular mechanisms of protein secretion, and they define the functions of SNAREs in BDNF signaling and regulation of neuronal connectivity. |
url |
http://www.sciencedirect.com/science/article/pii/S2211124715004337 |
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