Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus
Helianthus tuberosus is a potential source of resistance to many disease-provoking pathogens. Three accessions of H. tuberosus were used in this research and they were crossed with cultivated sunflower. Six F] and two BC1F1 hybrid combinations were obtained. Analysis of meiosis was performed using a...
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Serbian Genetics Society
2006-01-01
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Online Access: | http://www.doiserbia.nb.rs/img/doi/0534-0012/2006/0534-00120603203A.pdf |
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doaj-fce20d818a194c50a79a2f07be4175d72020-11-24T23:28:12ZengSerbian Genetics SocietyGenetika0534-00122006-01-0138320321310.2298/GENSR0603203ACytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuusAtlagić JovankaTerzić SretenHelianthus tuberosus is a potential source of resistance to many disease-provoking pathogens. Three accessions of H. tuberosus were used in this research and they were crossed with cultivated sunflower. Six F] and two BC1F1 hybrid combinations were obtained. Analysis of meiosis was performed using aceto-carmine method (GEORGIEVA-TODOROVA, 1976) and pollen viability was determined by staining method of ALEXANDER (1969). Meiosis was regular in cultivated sunflower and the pollen viability was high (96.8-98.9%). Low percent of irregularities was found in the meiosis of H. tuberosus. Pollen viability was high (97.2-98.7%). Chromosome pairing was mostly regular in F1 hybrids (34 bivalents), but some meiocytes contained 28-32 bivalents with uni- and quadrivalents present. The percent of meiocytes with fast chromosomes in metaphase was 24.6-87.2, with lagging chromosomes in anaphase I 10.5-81.0 and in telophase 25.0-33.3. Chromosome bridges were detected in 0-9.9% of meiocytes in anaphase. Pollen viability in F1 hybrids ranged from 27.0 to 47.9%. In BC1F1 hybrids number of bivalents was 16-25, univalent 2-18 and multivalent 0-1. Although a triploid set of chromosomes (51) was expected in BC1F1 hybrids, number of chromosomes was 45-57. Pollen viability varied from 0 to 54.3%. http://www.doiserbia.nb.rs/img/doi/0534-0012/2006/0534-00120603203A.pdfcultivated sunflowerHelianthus tuberosusinterspecific hybridizationmeiosispollen viability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Atlagić Jovanka Terzić Sreten |
spellingShingle |
Atlagić Jovanka Terzić Sreten Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus Genetika cultivated sunflower Helianthus tuberosus interspecific hybridization meiosis pollen viability |
author_facet |
Atlagić Jovanka Terzić Sreten |
author_sort |
Atlagić Jovanka |
title |
Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus |
title_short |
Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus |
title_full |
Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus |
title_fullStr |
Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus |
title_full_unstemmed |
Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus |
title_sort |
cytogenetic study of hexaploid species helianthus tuberosus and its f1 and bc1f1 hybrids with cultivated sunflower, h. annuus |
publisher |
Serbian Genetics Society |
series |
Genetika |
issn |
0534-0012 |
publishDate |
2006-01-01 |
description |
Helianthus tuberosus is a potential source of resistance to many disease-provoking pathogens. Three accessions of H. tuberosus were used in this research and they were crossed with cultivated sunflower. Six F] and two BC1F1 hybrid combinations were obtained. Analysis of meiosis was performed using aceto-carmine method (GEORGIEVA-TODOROVA, 1976) and pollen viability was determined by staining method of ALEXANDER (1969). Meiosis was regular in cultivated sunflower and the pollen viability was high (96.8-98.9%). Low percent of irregularities was found in the meiosis of H. tuberosus. Pollen viability was high (97.2-98.7%). Chromosome pairing was mostly regular in F1 hybrids (34 bivalents), but some meiocytes contained 28-32 bivalents with uni- and quadrivalents present. The percent of meiocytes with fast chromosomes in metaphase was 24.6-87.2, with lagging chromosomes in anaphase I 10.5-81.0 and in telophase 25.0-33.3. Chromosome bridges were detected in 0-9.9% of meiocytes in anaphase. Pollen viability in F1 hybrids ranged from 27.0 to 47.9%. In BC1F1 hybrids number of bivalents was 16-25, univalent 2-18 and multivalent 0-1. Although a triploid set of chromosomes (51) was expected in BC1F1 hybrids, number of chromosomes was 45-57. Pollen viability varied from 0 to 54.3%. |
topic |
cultivated sunflower Helianthus tuberosus interspecific hybridization meiosis pollen viability |
url |
http://www.doiserbia.nb.rs/img/doi/0534-0012/2006/0534-00120603203A.pdf |
work_keys_str_mv |
AT atlagicjovanka cytogeneticstudyofhexaploidspecieshelianthustuberosusanditsf1andbc1f1hybridswithcultivatedsunflowerhannuus AT terzicsreten cytogeneticstudyofhexaploidspecieshelianthustuberosusanditsf1andbc1f1hybridswithcultivatedsunflowerhannuus |
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