Attainment of brown adipocyte features in white adipocytes of hormone-sensitive lipase null mice.

BACKGROUND: Hormone-sensitive lipase (HSL) is expressed predominantly in adipose tissue, where it plays an important role in catecholamine-stimulated hydrolysis of stored tri- and diglycerides, thus mobilizing fatty acids. HSL exhibits broad substrate specificity and besides acylglycerides it hydrol...

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Main Authors: Kristoffer Ström, Ola Hansson, Stéphanie Lucas, Pernilla Nevsten, Céline Fernandez, Cecilia Klint, Sofia Movérare-Skrtic, Frank Sundler, Claes Ohlsson, Cecilia Holm
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2008-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2258419?pdf=render
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spelling doaj-fcea4ab05bc0418d8783b39a95f5aac22020-11-25T01:58:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-0133e179310.1371/journal.pone.0001793Attainment of brown adipocyte features in white adipocytes of hormone-sensitive lipase null mice.Kristoffer StrömOla HanssonStéphanie LucasPernilla NevstenCéline FernandezCecilia KlintSofia Movérare-SkrticFrank SundlerClaes OhlssonCecilia HolmBACKGROUND: Hormone-sensitive lipase (HSL) is expressed predominantly in adipose tissue, where it plays an important role in catecholamine-stimulated hydrolysis of stored tri- and diglycerides, thus mobilizing fatty acids. HSL exhibits broad substrate specificity and besides acylglycerides it hydrolyzes cholesteryl esters, retinyl esters and lipoidal esters. Despite its role in fatty acid mobilization, HSL null mice have been shown to be resistant to diet-induced obesity. METHODOLOGY/PRINCIPAL FINDINGS: Following a high-fat diet (HFD) regimen, energy expenditure, measured using indirect calorimetry, was increased in HSL null mice. White adipose tissue of HSL null mice was characterized by reduced mass and reduced protein expression of PPARgamma, a key transcription factor in adipogenesis, and stearoyl-CoA desaturase 1, the expression of which is known to be positively correlated to the differentiation state of the adipocyte. The protein expression of uncoupling protein-1 (UCP-1), the highly specific marker of brown adipocytes, was increased 7-fold in white adipose tissue of HSL null mice compared to wildtype littermates. Transmission electron microscopy revealed an increase in the size of mitochondria of white adipocytes of HSL null mice. The mRNA expression of pRb and RIP140 was decreased in isolated white adipocytes, while the expression of UCP-1 and CPT1 was increased in HSL null mice compared to wildtype littermates. Basal oxygen consumption was increased almost 3-fold in white adipose tissue of HSL null mice and was accompanied by increased uncoupling activity. CONCLUSIONS: These data suggest that HSL is involved in the determination of white versus brown adipocytes during adipocyte differentiation The exact mechanism(s) underlying this novel role of HSL remains to be elucidated, but it seems clear that HSL is required to sustain normal expression levels of pRb and RIP140, which both promote differentiation into the white, rather than the brown, adipocyte lineage.http://europepmc.org/articles/PMC2258419?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Kristoffer Ström
Ola Hansson
Stéphanie Lucas
Pernilla Nevsten
Céline Fernandez
Cecilia Klint
Sofia Movérare-Skrtic
Frank Sundler
Claes Ohlsson
Cecilia Holm
spellingShingle Kristoffer Ström
Ola Hansson
Stéphanie Lucas
Pernilla Nevsten
Céline Fernandez
Cecilia Klint
Sofia Movérare-Skrtic
Frank Sundler
Claes Ohlsson
Cecilia Holm
Attainment of brown adipocyte features in white adipocytes of hormone-sensitive lipase null mice.
PLoS ONE
author_facet Kristoffer Ström
Ola Hansson
Stéphanie Lucas
Pernilla Nevsten
Céline Fernandez
Cecilia Klint
Sofia Movérare-Skrtic
Frank Sundler
Claes Ohlsson
Cecilia Holm
author_sort Kristoffer Ström
title Attainment of brown adipocyte features in white adipocytes of hormone-sensitive lipase null mice.
title_short Attainment of brown adipocyte features in white adipocytes of hormone-sensitive lipase null mice.
title_full Attainment of brown adipocyte features in white adipocytes of hormone-sensitive lipase null mice.
title_fullStr Attainment of brown adipocyte features in white adipocytes of hormone-sensitive lipase null mice.
title_full_unstemmed Attainment of brown adipocyte features in white adipocytes of hormone-sensitive lipase null mice.
title_sort attainment of brown adipocyte features in white adipocytes of hormone-sensitive lipase null mice.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2008-01-01
description BACKGROUND: Hormone-sensitive lipase (HSL) is expressed predominantly in adipose tissue, where it plays an important role in catecholamine-stimulated hydrolysis of stored tri- and diglycerides, thus mobilizing fatty acids. HSL exhibits broad substrate specificity and besides acylglycerides it hydrolyzes cholesteryl esters, retinyl esters and lipoidal esters. Despite its role in fatty acid mobilization, HSL null mice have been shown to be resistant to diet-induced obesity. METHODOLOGY/PRINCIPAL FINDINGS: Following a high-fat diet (HFD) regimen, energy expenditure, measured using indirect calorimetry, was increased in HSL null mice. White adipose tissue of HSL null mice was characterized by reduced mass and reduced protein expression of PPARgamma, a key transcription factor in adipogenesis, and stearoyl-CoA desaturase 1, the expression of which is known to be positively correlated to the differentiation state of the adipocyte. The protein expression of uncoupling protein-1 (UCP-1), the highly specific marker of brown adipocytes, was increased 7-fold in white adipose tissue of HSL null mice compared to wildtype littermates. Transmission electron microscopy revealed an increase in the size of mitochondria of white adipocytes of HSL null mice. The mRNA expression of pRb and RIP140 was decreased in isolated white adipocytes, while the expression of UCP-1 and CPT1 was increased in HSL null mice compared to wildtype littermates. Basal oxygen consumption was increased almost 3-fold in white adipose tissue of HSL null mice and was accompanied by increased uncoupling activity. CONCLUSIONS: These data suggest that HSL is involved in the determination of white versus brown adipocytes during adipocyte differentiation The exact mechanism(s) underlying this novel role of HSL remains to be elucidated, but it seems clear that HSL is required to sustain normal expression levels of pRb and RIP140, which both promote differentiation into the white, rather than the brown, adipocyte lineage.
url http://europepmc.org/articles/PMC2258419?pdf=render
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