Isolation and Mechanical Measurements of Myofibrils from Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Summary: Tension production and contractile properties are poorly characterized aspects of excitation-contraction coupling of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Previous approaches have been limited due to the small size and structural immaturity of early-stage h...
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Format: | Article |
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Elsevier
2016-06-01
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Series: | Stem Cell Reports |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671116300315 |
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doaj-fcea7251262e4c4781ea99c51aaabcc4 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Josè Manuel Pioner Alice W. Racca Jordan M. Klaiman Kai-Chun Yang Xuan Guan Lil Pabon Veronica Muskheli Rebecca Zaunbrecher Jesse Macadangdang Mark Y. Jeong David L. Mack Martin K. Childers Deok-Ho Kim Chiara Tesi Corrado Poggesi Charles E. Murry Michael Regnier |
spellingShingle |
Josè Manuel Pioner Alice W. Racca Jordan M. Klaiman Kai-Chun Yang Xuan Guan Lil Pabon Veronica Muskheli Rebecca Zaunbrecher Jesse Macadangdang Mark Y. Jeong David L. Mack Martin K. Childers Deok-Ho Kim Chiara Tesi Corrado Poggesi Charles E. Murry Michael Regnier Isolation and Mechanical Measurements of Myofibrils from Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Stem Cell Reports |
author_facet |
Josè Manuel Pioner Alice W. Racca Jordan M. Klaiman Kai-Chun Yang Xuan Guan Lil Pabon Veronica Muskheli Rebecca Zaunbrecher Jesse Macadangdang Mark Y. Jeong David L. Mack Martin K. Childers Deok-Ho Kim Chiara Tesi Corrado Poggesi Charles E. Murry Michael Regnier |
author_sort |
Josè Manuel Pioner |
title |
Isolation and Mechanical Measurements of Myofibrils from Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes |
title_short |
Isolation and Mechanical Measurements of Myofibrils from Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes |
title_full |
Isolation and Mechanical Measurements of Myofibrils from Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes |
title_fullStr |
Isolation and Mechanical Measurements of Myofibrils from Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes |
title_full_unstemmed |
Isolation and Mechanical Measurements of Myofibrils from Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes |
title_sort |
isolation and mechanical measurements of myofibrils from human induced pluripotent stem cell-derived cardiomyocytes |
publisher |
Elsevier |
series |
Stem Cell Reports |
issn |
2213-6711 |
publishDate |
2016-06-01 |
description |
Summary: Tension production and contractile properties are poorly characterized aspects of excitation-contraction coupling of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Previous approaches have been limited due to the small size and structural immaturity of early-stage hiPSC-CMs. We developed a substrate nanopatterning approach to produce hiPSC-CMs in culture with adult-like dimensions, T-tubule-like structures, and aligned myofibrils. We then isolated myofibrils from hiPSC-CMs and measured the tension and kinetics of activation and relaxation using a custom-built apparatus with fast solution switching. The contractile properties and ultrastructure of myofibrils more closely resembled human fetal myofibrils of similar gestational age than adult preparations. We also demonstrated the ability to study the development of contractile dysfunction of myofibrils from a patient-derived hiPSC-CM cell line carrying the familial cardiomyopathy MYH7 mutation (E848G). These methods can bring new insights to understanding cardiomyocyte maturation and developmental mechanical dysfunction of hiPSC-CMs with cardiomyopathic mutations. : In this article, Pioner and colleagues reported contractile properties of isolated myofibrils from hiPSC-CMs with highly mature morphology. This approach permits quantitative assessment of maturation and contractile properties of hiPSC-CMs and can be used to study the development of contractile dysfunction in genetically based cardiac diseases. The authors present a patient-derived cell line carrying a novel familial cardiomyopathy MYH7 mutation (E848G). |
url |
http://www.sciencedirect.com/science/article/pii/S2213671116300315 |
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doaj-fcea7251262e4c4781ea99c51aaabcc42020-11-24T21:23:47ZengElsevierStem Cell Reports2213-67112016-06-0166885896Isolation and Mechanical Measurements of Myofibrils from Human Induced Pluripotent Stem Cell-Derived CardiomyocytesJosè Manuel Pioner0Alice W. Racca1Jordan M. Klaiman2Kai-Chun Yang3Xuan Guan4Lil Pabon5Veronica Muskheli6Rebecca Zaunbrecher7Jesse Macadangdang8Mark Y. Jeong9David L. Mack10Martin K. Childers11Deok-Ho Kim12Chiara Tesi13Corrado Poggesi14Charles E. Murry15Michael Regnier16Division of Physiology, Department of Experimental and Clinical Medicine, University of Florence, Viale Morgagni 63, 50134 Florence, Italy; Corresponding authorBioengineering, University of Washington, Seattle, WA 98109, USABioengineering, University of Washington, Seattle, WA 98109, USAMedicine, Division of Cardiology, University of Washington, Seattle, WA 98195, USARehabilitation Medicine, University of Washington, Seattle, WA 98109, USAPathology, University of Washington, Seattle, WA 98109, USAPathology, University of Washington, Seattle, WA 98109, USABioengineering, University of Washington, Seattle, WA 98109, USABioengineering, University of Washington, Seattle, WA 98109, USAMedicine, Division of Cardiology, University of Colorado, Denver, CO 80262, USARehabilitation Medicine, University of Washington, Seattle, WA 98109, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USARehabilitation Medicine, University of Washington, Seattle, WA 98109, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USABioengineering, University of Washington, Seattle, WA 98109, USADivision of Physiology, Department of Experimental and Clinical Medicine, University of Florence, Viale Morgagni 63, 50134 Florence, ItalyDivision of Physiology, Department of Experimental and Clinical Medicine, University of Florence, Viale Morgagni 63, 50134 Florence, ItalyBioengineering, University of Washington, Seattle, WA 98109, USA; Medicine, Division of Cardiology, University of Washington, Seattle, WA 98195, USA; Pathology, University of Washington, Seattle, WA 98109, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA; Center for Cardiovascular Biology, University of Washington, Seattle, WA 98109, USABioengineering, University of Washington, Seattle, WA 98109, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA; Center for Cardiovascular Biology, University of Washington, Seattle, WA 98109, USASummary: Tension production and contractile properties are poorly characterized aspects of excitation-contraction coupling of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Previous approaches have been limited due to the small size and structural immaturity of early-stage hiPSC-CMs. We developed a substrate nanopatterning approach to produce hiPSC-CMs in culture with adult-like dimensions, T-tubule-like structures, and aligned myofibrils. We then isolated myofibrils from hiPSC-CMs and measured the tension and kinetics of activation and relaxation using a custom-built apparatus with fast solution switching. The contractile properties and ultrastructure of myofibrils more closely resembled human fetal myofibrils of similar gestational age than adult preparations. We also demonstrated the ability to study the development of contractile dysfunction of myofibrils from a patient-derived hiPSC-CM cell line carrying the familial cardiomyopathy MYH7 mutation (E848G). These methods can bring new insights to understanding cardiomyocyte maturation and developmental mechanical dysfunction of hiPSC-CMs with cardiomyopathic mutations. : In this article, Pioner and colleagues reported contractile properties of isolated myofibrils from hiPSC-CMs with highly mature morphology. This approach permits quantitative assessment of maturation and contractile properties of hiPSC-CMs and can be used to study the development of contractile dysfunction in genetically based cardiac diseases. The authors present a patient-derived cell line carrying a novel familial cardiomyopathy MYH7 mutation (E848G).http://www.sciencedirect.com/science/article/pii/S2213671116300315 |