Cytotoxic Effect of Hypoxic Environment in Mesenchymal Stem Cell
Bone marrow mesenchymal stem cells (BM-MSCs) are used to repair hypoxic or ischemic tissue. After hypoxic the level of ATP is decreases, cellular functions do not continue and apoptosis or necrosis occur. Apoptosis is a progress of programmed cell death that occurs in normal or pathological conditio...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-12-01
|
Series: | Proceedings |
Subjects: | |
Online Access: | https://www.mdpi.com/2504-3900/2/25/1592 |
id |
doaj-fcf623f26a124494a8e9d3f32e9affc3 |
---|---|
record_format |
Article |
spelling |
doaj-fcf623f26a124494a8e9d3f32e9affc32020-11-24T23:31:29ZengMDPI AGProceedings2504-39002018-12-01225159210.3390/proceedings2251592proceedings2251592Cytotoxic Effect of Hypoxic Environment in Mesenchymal Stem CellSevil Özer0H. Seda Vatansever1Feyzan Özdal-Kurt2Faculty of Sciences and Letters, Department of Biology, Manisa Celal Bayar University, Manisa 45140, TurkeyFaculty of Medicine, Department of Histology and Embryology, Manisa Celal Bayar University, Manisa 45020, TurkeyFaculty of Sciences and Letters, Department of Biology, Manisa Celal Bayar University, Manisa 45140, TurkeyBone marrow mesenchymal stem cells (BM-MSCs) are used to repair hypoxic or ischemic tissue. After hypoxic the level of ATP is decreases, cellular functions do not continue and apoptosis or necrosis occur. Apoptosis is a progress of programmed cell death that occurs in normal or pathological conditions. In this study, we were investigated the hypoxic effect on apoptosis in mesenchymal stem cell. Bone marrow-derived stem cells were cultured in hypoxic (1% or 3%) or normoxic conditions 24, 96 well plates for 36 h. Cell viability was shown by MTT assay on 36 h. After fixation of cells with 4% paraformaldehyde, distributions of caspase-3, Bcl-2 and Bax with indirect immunoperoxidase technique, apoptotic cells with TUNEL assay were investigated. All staining results were evaluated using H-score analyses method with ANOVA, statistically. As a result, hypoxic condition was toxic for human mesenchymal stem cells and the number of death cell was higher in that than normoxic condition.https://www.mdpi.com/2504-3900/2/25/1592stem cellshypoxiccytotoxicity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sevil Özer H. Seda Vatansever Feyzan Özdal-Kurt |
spellingShingle |
Sevil Özer H. Seda Vatansever Feyzan Özdal-Kurt Cytotoxic Effect of Hypoxic Environment in Mesenchymal Stem Cell Proceedings stem cells hypoxic cytotoxicity |
author_facet |
Sevil Özer H. Seda Vatansever Feyzan Özdal-Kurt |
author_sort |
Sevil Özer |
title |
Cytotoxic Effect of Hypoxic Environment in Mesenchymal Stem Cell |
title_short |
Cytotoxic Effect of Hypoxic Environment in Mesenchymal Stem Cell |
title_full |
Cytotoxic Effect of Hypoxic Environment in Mesenchymal Stem Cell |
title_fullStr |
Cytotoxic Effect of Hypoxic Environment in Mesenchymal Stem Cell |
title_full_unstemmed |
Cytotoxic Effect of Hypoxic Environment in Mesenchymal Stem Cell |
title_sort |
cytotoxic effect of hypoxic environment in mesenchymal stem cell |
publisher |
MDPI AG |
series |
Proceedings |
issn |
2504-3900 |
publishDate |
2018-12-01 |
description |
Bone marrow mesenchymal stem cells (BM-MSCs) are used to repair hypoxic or ischemic tissue. After hypoxic the level of ATP is decreases, cellular functions do not continue and apoptosis or necrosis occur. Apoptosis is a progress of programmed cell death that occurs in normal or pathological conditions. In this study, we were investigated the hypoxic effect on apoptosis in mesenchymal stem cell. Bone marrow-derived stem cells were cultured in hypoxic (1% or 3%) or normoxic conditions 24, 96 well plates for 36 h. Cell viability was shown by MTT assay on 36 h. After fixation of cells with 4% paraformaldehyde, distributions of caspase-3, Bcl-2 and Bax with indirect immunoperoxidase technique, apoptotic cells with TUNEL assay were investigated. All staining results were evaluated using H-score analyses method with ANOVA, statistically. As a result, hypoxic condition was toxic for human mesenchymal stem cells and the number of death cell was higher in that than normoxic condition. |
topic |
stem cells hypoxic cytotoxicity |
url |
https://www.mdpi.com/2504-3900/2/25/1592 |
work_keys_str_mv |
AT sevilozer cytotoxiceffectofhypoxicenvironmentinmesenchymalstemcell AT hsedavatansever cytotoxiceffectofhypoxicenvironmentinmesenchymalstemcell AT feyzanozdalkurt cytotoxiceffectofhypoxicenvironmentinmesenchymalstemcell |
_version_ |
1725537802581442560 |