Cytotoxic Effect of Hypoxic Environment in Mesenchymal Stem Cell

• Main Authors: Sevil Özer, H. Seda Vatansever, Feyzan Özdal-Kurt
• Format: Article
• Language: English
• Published: MDPI AG 2018-12-01
• Series: Proceedings
• Subjects: stem cells; hypoxic; cytotoxicity
• Online Access: https://www.mdpi.com/2504-3900/2/25/1592

Bone marrow mesenchymal stem cells (BM-MSCs) are used to repair hypoxic or ischemic tissue. After hypoxic the level of ATP is decreases, cellular functions do not continue and apoptosis or necrosis occur. Apoptosis is a progress of programmed cell death that occurs in normal or pathological conditions. In this study, we were investigated the hypoxic effect on apoptosis in mesenchymal stem cell. Bone marrow-derived stem cells were cultured in hypoxic (1% or 3%) or normoxic conditions 24, 96 well plates for 36 h. Cell viability was shown by MTT assay on 36 h. After fixation of cells with 4% paraformaldehyde, distributions of caspase-3, Bcl-2 and Bax with indirect immunoperoxidase technique, apoptotic cells with TUNEL assay were investigated. All staining results were evaluated using H-score analyses method with ANOVA, statistically. As a result, hypoxic condition was toxic for human mesenchymal stem cells and the number of death cell was higher in that than normoxic condition.