Genotypic Characterization of Rickettsia bellii Reveals Distinct Lineages in the United States and South America
The bacterium Rickettsia bellii belongs to a basal group of rickettsiae that diverged prior to the pathogenic spotted fever group and typhus group Rickettsia species. Despite a diverse representation of R. bellii across more than 25 species of hard and soft ticks in the American continent, phylogeog...
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doaj-fcf9fdcc15ac4fd4977622875654c5612020-11-24T23:14:59ZengHindawi LimitedBioMed Research International2314-61332314-61412018-01-01201810.1155/2018/85054838505483Genotypic Characterization of Rickettsia bellii Reveals Distinct Lineages in the United States and South AmericaFelipe S. Krawczak0Marcelo B. Labruna1Joy A. Hecht2Christopher D. Paddock3Sandor E. Karpathy4Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, São Paulo, SP, BrazilDepartment of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, São Paulo, SP, BrazilRickettsial Zoonoses Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USARickettsial Zoonoses Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USARickettsial Zoonoses Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USAThe bacterium Rickettsia bellii belongs to a basal group of rickettsiae that diverged prior to the pathogenic spotted fever group and typhus group Rickettsia species. Despite a diverse representation of R. bellii across more than 25 species of hard and soft ticks in the American continent, phylogeographical relationships among strains of this basal group-Rickettsia species are unknown; the work described here explores these relationships. DNA was extracted from 30 R. bellii tick isolates: 15 from the United States, 14 from Brazil, and 1 from Argentina. A total of 2,269 aligned nucleotide sites of 3 protein coding genes (gltA, atpA, and coxA) and 2 intergenic regions (rpmE-tRNAfmet and RC1027-xthA2) were concatenated and subjected to phylogenetic analysis by Bayesian methods. Results showed a separation of almost all isolates between North and South Americas, suggesting that they have radiated within their respective continents. Phylogenetic positions of the 30 isolates could be a result of not only their geographical origin but also the tick hosts they have coevolved with. Whether R. bellii originated with ticks in North or South America remains obscure, as our analyses did not show evidence for greater genetic divergence of R. bellii in either continent.http://dx.doi.org/10.1155/2018/8505483 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Felipe S. Krawczak Marcelo B. Labruna Joy A. Hecht Christopher D. Paddock Sandor E. Karpathy |
spellingShingle |
Felipe S. Krawczak Marcelo B. Labruna Joy A. Hecht Christopher D. Paddock Sandor E. Karpathy Genotypic Characterization of Rickettsia bellii Reveals Distinct Lineages in the United States and South America BioMed Research International |
author_facet |
Felipe S. Krawczak Marcelo B. Labruna Joy A. Hecht Christopher D. Paddock Sandor E. Karpathy |
author_sort |
Felipe S. Krawczak |
title |
Genotypic Characterization of Rickettsia bellii Reveals Distinct Lineages in the United States and South America |
title_short |
Genotypic Characterization of Rickettsia bellii Reveals Distinct Lineages in the United States and South America |
title_full |
Genotypic Characterization of Rickettsia bellii Reveals Distinct Lineages in the United States and South America |
title_fullStr |
Genotypic Characterization of Rickettsia bellii Reveals Distinct Lineages in the United States and South America |
title_full_unstemmed |
Genotypic Characterization of Rickettsia bellii Reveals Distinct Lineages in the United States and South America |
title_sort |
genotypic characterization of rickettsia bellii reveals distinct lineages in the united states and south america |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2018-01-01 |
description |
The bacterium Rickettsia bellii belongs to a basal group of rickettsiae that diverged prior to the pathogenic spotted fever group and typhus group Rickettsia species. Despite a diverse representation of R. bellii across more than 25 species of hard and soft ticks in the American continent, phylogeographical relationships among strains of this basal group-Rickettsia species are unknown; the work described here explores these relationships. DNA was extracted from 30 R. bellii tick isolates: 15 from the United States, 14 from Brazil, and 1 from Argentina. A total of 2,269 aligned nucleotide sites of 3 protein coding genes (gltA, atpA, and coxA) and 2 intergenic regions (rpmE-tRNAfmet and RC1027-xthA2) were concatenated and subjected to phylogenetic analysis by Bayesian methods. Results showed a separation of almost all isolates between North and South Americas, suggesting that they have radiated within their respective continents. Phylogenetic positions of the 30 isolates could be a result of not only their geographical origin but also the tick hosts they have coevolved with. Whether R. bellii originated with ticks in North or South America remains obscure, as our analyses did not show evidence for greater genetic divergence of R. bellii in either continent. |
url |
http://dx.doi.org/10.1155/2018/8505483 |
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