Brain astrocyte synthesis of docosahexaenoic acid from n-3 fatty acids is limited at the elongation of docosapentaenoic acid
The phospholipids, particularly phosphatidylethanolamine, of brain gray matter are enriched with docosahexaenoic acid (22:6n-3). The importance of uptake of preformed 22:6n-3 from plasma compared with synthesis from the α-linolenic acid (18:3n-3) precursor in brain is not known. Deficiency of 18:3n-...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2002-09-01
|
Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520328170 |
id |
doaj-fcfe16b1e241497dbbf461bdf4368d0a |
---|---|
record_format |
Article |
spelling |
doaj-fcfe16b1e241497dbbf461bdf4368d0a2021-04-27T04:43:12ZengElsevierJournal of Lipid Research0022-22752002-09-0143915291536Brain astrocyte synthesis of docosahexaenoic acid from n-3 fatty acids is limited at the elongation of docosapentaenoic acidSheila M. Innis0Roger A. Dyer1Department of Paediatrics, University of British Columbia, Vancouver, BC, Canada, V5Z 4H4Department of Paediatrics, University of British Columbia, Vancouver, BC, Canada, V5Z 4H4The phospholipids, particularly phosphatidylethanolamine, of brain gray matter are enriched with docosahexaenoic acid (22:6n-3). The importance of uptake of preformed 22:6n-3 from plasma compared with synthesis from the α-linolenic acid (18:3n-3) precursor in brain is not known. Deficiency of 18:3n-3 results in a compensatory increase in the n-6 docosapentaenoic acid (22:5n-6) in brain, which could be formed from the precursor linoleic acid (18:2n-6) in liver or brain. We studied n-3 and n-6 fatty acid incorporation in brain astrocytes cultured in chemically defined medium using delipidated serum supplemented with specific fatty acids. High performance liquid chromatography with evaporative light scattering detection and gas liquid chromatography were used to separate and quantify cell and media lipids and fatty acids. Although astrocytes are able to form 22:6n-3, incubation with 18:3n-3 or eicosapentaenoic acid (20:5n-3) resulted in a time and concentration dependent accumulation of 22:5n-3 and decrease in 22:6n-3 g/g cell fatty acids. Astrocytes cultured with 18:2n-6 failed to accumulate 22:5n-6.Astrocytes secreted cholesterol esters (CE) and phosphatidylethanolamine containing saturated and monounsaturated fatty acids, and arachidonic acid (20:4n-6) and 22:6n-3. These studies suggest conversion of 22:5n-3 limits 22:6n-3 synthesis, and show astrocytes release fatty acids in CE.http://www.sciencedirect.com/science/article/pii/S0022227520328170astrocytedocosahexaenoic aciddocosapentaenoic acidalpha linolenic aciddesaturationelongation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sheila M. Innis Roger A. Dyer |
spellingShingle |
Sheila M. Innis Roger A. Dyer Brain astrocyte synthesis of docosahexaenoic acid from n-3 fatty acids is limited at the elongation of docosapentaenoic acid Journal of Lipid Research astrocyte docosahexaenoic acid docosapentaenoic acid alpha linolenic acid desaturation elongation |
author_facet |
Sheila M. Innis Roger A. Dyer |
author_sort |
Sheila M. Innis |
title |
Brain astrocyte synthesis of docosahexaenoic acid from n-3 fatty acids is limited at the elongation of docosapentaenoic acid |
title_short |
Brain astrocyte synthesis of docosahexaenoic acid from n-3 fatty acids is limited at the elongation of docosapentaenoic acid |
title_full |
Brain astrocyte synthesis of docosahexaenoic acid from n-3 fatty acids is limited at the elongation of docosapentaenoic acid |
title_fullStr |
Brain astrocyte synthesis of docosahexaenoic acid from n-3 fatty acids is limited at the elongation of docosapentaenoic acid |
title_full_unstemmed |
Brain astrocyte synthesis of docosahexaenoic acid from n-3 fatty acids is limited at the elongation of docosapentaenoic acid |
title_sort |
brain astrocyte synthesis of docosahexaenoic acid from n-3 fatty acids is limited at the elongation of docosapentaenoic acid |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2002-09-01 |
description |
The phospholipids, particularly phosphatidylethanolamine, of brain gray matter are enriched with docosahexaenoic acid (22:6n-3). The importance of uptake of preformed 22:6n-3 from plasma compared with synthesis from the α-linolenic acid (18:3n-3) precursor in brain is not known. Deficiency of 18:3n-3 results in a compensatory increase in the n-6 docosapentaenoic acid (22:5n-6) in brain, which could be formed from the precursor linoleic acid (18:2n-6) in liver or brain. We studied n-3 and n-6 fatty acid incorporation in brain astrocytes cultured in chemically defined medium using delipidated serum supplemented with specific fatty acids. High performance liquid chromatography with evaporative light scattering detection and gas liquid chromatography were used to separate and quantify cell and media lipids and fatty acids. Although astrocytes are able to form 22:6n-3, incubation with 18:3n-3 or eicosapentaenoic acid (20:5n-3) resulted in a time and concentration dependent accumulation of 22:5n-3 and decrease in 22:6n-3 g/g cell fatty acids. Astrocytes cultured with 18:2n-6 failed to accumulate 22:5n-6.Astrocytes secreted cholesterol esters (CE) and phosphatidylethanolamine containing saturated and monounsaturated fatty acids, and arachidonic acid (20:4n-6) and 22:6n-3. These studies suggest conversion of 22:5n-3 limits 22:6n-3 synthesis, and show astrocytes release fatty acids in CE. |
topic |
astrocyte docosahexaenoic acid docosapentaenoic acid alpha linolenic acid desaturation elongation |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520328170 |
work_keys_str_mv |
AT sheilaminnis brainastrocytesynthesisofdocosahexaenoicacidfromn3fattyacidsislimitedattheelongationofdocosapentaenoicacid AT rogeradyer brainastrocytesynthesisofdocosahexaenoicacidfromn3fattyacidsislimitedattheelongationofdocosapentaenoicacid |
_version_ |
1721506752292192256 |