Effect of Paternal DNA Damage on Paternal DNA Degradation and Early Embryonic Development in Mouse Embryo: Supporting Evidence by GammaH2AX Expression
This study was investigated to test whether the zygote recognized the topoisomerase II beta (TOP2B) mediated DNA fragmentation in epididymal spermatozoa or the nuclease degradation in vas deferens spermatozoa by testing for the presence of gammaH2AX (γH2AX). The γH2AX is phosphorylation of histone p...
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The Korean Society of Animal Reproduction and Biotechnology
2019-09-01
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doaj-fd151306be1f46808bf1e442ae68713d2020-11-25T03:32:37ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632019-09-0134319720410.12750/JARB.34.3.197Effect of Paternal DNA Damage on Paternal DNA Degradation and Early Embryonic Development in Mouse Embryo: Supporting Evidence by GammaH2AX ExpressionChang Jin Kim0Kyung-Bon Lee1https://orcid.org/0000-0001-9825-8891Department of Biology Education, College of Education, Chonnam National University, Gwangju 61186, KoreaDepartment of Biology Education, College of Education, Chonnam National University, Gwangju 61186, KoreaThis study was investigated to test whether the zygote recognized the topoisomerase II beta (TOP2B) mediated DNA fragmentation in epididymal spermatozoa or the nuclease degradation in vas deferens spermatozoa by testing for the presence of gammaH2AX (γH2AX). The γH2AX is phosphorylation of histone protein H2AX on serine 139 occurs at sites flanking DNA double-stranded breaks (DSBs). The presence of γH2AX in the pronuclei of mouse zygotes which were injected with DNA broke epididymal spermatozoa was tested by immunohistochemistry at 5 and 9 h post fertilization, respectively. Paternal pronuclei that arose from epididymal spermatozoa treated with divalent cations did not stain for γH2AX at 5 h. On the other hand, in embryos injected with vas deferences spermatozoa that had been treated with divalent cations, γH2AX was only present in paternal pronuclei, and not the maternal pronuclei at 5 h. Interestingly, both pronuclei stained positively for γH2AX for all treatments and controls at 9 h after sperm injection. In conclusion, the embryos recognize DNA that is damaged by nuclease, but not by TOP2B because H2AX in phosphorylated in paternal pronuclei resulting from spermatozoa treated with fragmented DNA from vas deferens spermatozoa treated with divalent cations, but not from epididymal spermatozoa treated the same way.http://www.e-jarb.org/journal/view.html?uid=2483&vmd=Fulldna degradationdna fragmentationdna licensingdna replicationgammah2ax |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chang Jin Kim Kyung-Bon Lee |
spellingShingle |
Chang Jin Kim Kyung-Bon Lee Effect of Paternal DNA Damage on Paternal DNA Degradation and Early Embryonic Development in Mouse Embryo: Supporting Evidence by GammaH2AX Expression Journal of Animal Reproduction and Biotechnology dna degradation dna fragmentation dna licensing dna replication gammah2ax |
author_facet |
Chang Jin Kim Kyung-Bon Lee |
author_sort |
Chang Jin Kim |
title |
Effect of Paternal DNA Damage on Paternal DNA Degradation and Early Embryonic Development in Mouse Embryo: Supporting Evidence by GammaH2AX Expression |
title_short |
Effect of Paternal DNA Damage on Paternal DNA Degradation and Early Embryonic Development in Mouse Embryo: Supporting Evidence by GammaH2AX Expression |
title_full |
Effect of Paternal DNA Damage on Paternal DNA Degradation and Early Embryonic Development in Mouse Embryo: Supporting Evidence by GammaH2AX Expression |
title_fullStr |
Effect of Paternal DNA Damage on Paternal DNA Degradation and Early Embryonic Development in Mouse Embryo: Supporting Evidence by GammaH2AX Expression |
title_full_unstemmed |
Effect of Paternal DNA Damage on Paternal DNA Degradation and Early Embryonic Development in Mouse Embryo: Supporting Evidence by GammaH2AX Expression |
title_sort |
effect of paternal dna damage on paternal dna degradation and early embryonic development in mouse embryo: supporting evidence by gammah2ax expression |
publisher |
The Korean Society of Animal Reproduction and Biotechnology |
series |
Journal of Animal Reproduction and Biotechnology |
issn |
2671-4639 2671-4663 |
publishDate |
2019-09-01 |
description |
This study was investigated to test whether the zygote recognized the topoisomerase II beta (TOP2B) mediated DNA fragmentation in epididymal spermatozoa or the nuclease degradation in vas deferens spermatozoa by testing for the presence of gammaH2AX (γH2AX). The γH2AX is phosphorylation of histone protein H2AX on serine 139 occurs at sites flanking DNA double-stranded breaks (DSBs). The presence of γH2AX in the pronuclei of mouse zygotes which were injected with DNA broke epididymal spermatozoa was tested by immunohistochemistry at 5 and 9 h post fertilization, respectively. Paternal pronuclei that arose from epididymal spermatozoa treated with divalent cations did not stain for γH2AX at 5 h. On the other hand, in embryos injected with vas deferences spermatozoa that had been treated with divalent cations, γH2AX was only present in paternal pronuclei, and not the maternal pronuclei at 5 h. Interestingly, both pronuclei stained positively for γH2AX for all treatments and controls at 9 h after sperm injection. In conclusion, the embryos recognize DNA that is damaged by nuclease, but not by TOP2B because H2AX in phosphorylated in paternal pronuclei resulting from spermatozoa treated with fragmented DNA from vas deferens spermatozoa treated with divalent cations, but not from epididymal spermatozoa treated the same way. |
topic |
dna degradation dna fragmentation dna licensing dna replication gammah2ax |
url |
http://www.e-jarb.org/journal/view.html?uid=2483&vmd=Full |
work_keys_str_mv |
AT changjinkim effectofpaternaldnadamageonpaternaldnadegradationandearlyembryonicdevelopmentinmouseembryosupportingevidencebygammah2axexpression AT kyungbonlee effectofpaternaldnadamageonpaternaldnadegradationandearlyembryonicdevelopmentinmouseembryosupportingevidencebygammah2axexpression |
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