Genomic Regions That Underlie Soybean Seed Isoflavone Content
Soy products contain isoflavones (genistein, daidzein, and glycitein) that display biological effects when ingested by humans and animals, these effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect. Our objecti...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2001-01-01
|
Series: | Journal of Biomedicine and Biotechnology |
Online Access: | http://dx.doi.org/10.1155/S1110724301000110 |
id |
doaj-fd21e32197c64b13ba58ab9c359ada18 |
---|---|
record_format |
Article |
spelling |
doaj-fd21e32197c64b13ba58ab9c359ada182020-11-25T01:57:02ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512001-01-0111384410.1155/S1110724301000110Genomic Regions That Underlie Soybean Seed Isoflavone ContentK. Meksem0V. N. Njiti1W. J. Banz2M. J. Iqbal3My. M. Kassem4D. L. Hyten5J. Yuang6T. A. Winters7D. A. Lightfoot8Department of Plant, Soil and General Agriculture, Southern Illinois University at Carbondale, Ag Building Room 176, Carbondale 62901-4415, IL, USADepartment of Plant, Soil and General Agriculture, Southern Illinois University at Carbondale, Ag Building Room 176, Carbondale 62901-4415, IL, USADepartment of Animal Science, Food and Nutrition, Southern Illinois University at Carbondale, Carbondale 62901-4417, IL, USADepartment of Plant, Soil and General Agriculture, Southern Illinois University at Carbondale, Ag Building Room 176, Carbondale 62901-4415, IL, USADepartment of Plant, Soil and General Agriculture, Southern Illinois University at Carbondale, Ag Building Room 176, Carbondale 62901-4415, IL, USADepartment of Plant, Soil and General Agriculture, Southern Illinois University at Carbondale, Ag Building Room 176, Carbondale 62901-4415, IL, USADepartment of Plant, Soil and General Agriculture, Southern Illinois University at Carbondale, Ag Building Room 176, Carbondale 62901-4415, IL, USADepartment of Animal Science, Food and Nutrition, Southern Illinois University at Carbondale, Carbondale 62901-4417, IL, USADepartment of Plant, Soil and General Agriculture, Southern Illinois University at Carbondale, Ag Building Room 176, Carbondale 62901-4415, IL, USASoy products contain isoflavones (genistein, daidzein, and glycitein) that display biological effects when ingested by humans and animals, these effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect. Our objective was to identify loci that underlie isoflavone content in soybean seeds. The study involved 100 recombinant inbred lines (RIL) from the cross of ‘Essex’ by ‘Forrest,’ two cultivars that contrast for isoflavone content. Isoflavone content of seeds from each RIL was determined by high performance liquid chromatography (HPLC). The distribution of isoflavone content was continuous and unimodal. The heritability estimates on a line mean basis were 79% for daidzein, 22% for genistein, and 88% for glycitein. Isoflavone content of soybean seeds was compared against 150 polymorphic DNA markers in a one-way analysis of variance. Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years. Molecular linkage group B1 contained a major QTL underlying glycitein content (P=0.0001,R 2=50.2%), linkage group N contained a QTL for glycitein (P=0.0033,R 2=11.1%) and a QTL for daidzein (P=0.0023,R 2=10.3%) and linkage group A1 contained a QTL for daidzein (P=0.0081,R 2=9.6%). Selection for these chromosomal regions in a marker assisted selection program will allow for the manipulation of amounts and profiles of isoflavones (genistein, daidzein, and glycitein) content of soybean seeds. In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.http://dx.doi.org/10.1155/S1110724301000110 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
K. Meksem V. N. Njiti W. J. Banz M. J. Iqbal My. M. Kassem D. L. Hyten J. Yuang T. A. Winters D. A. Lightfoot |
spellingShingle |
K. Meksem V. N. Njiti W. J. Banz M. J. Iqbal My. M. Kassem D. L. Hyten J. Yuang T. A. Winters D. A. Lightfoot Genomic Regions That Underlie Soybean Seed Isoflavone Content Journal of Biomedicine and Biotechnology |
author_facet |
K. Meksem V. N. Njiti W. J. Banz M. J. Iqbal My. M. Kassem D. L. Hyten J. Yuang T. A. Winters D. A. Lightfoot |
author_sort |
K. Meksem |
title |
Genomic Regions That Underlie Soybean Seed Isoflavone Content |
title_short |
Genomic Regions That Underlie Soybean Seed Isoflavone Content |
title_full |
Genomic Regions That Underlie Soybean Seed Isoflavone Content |
title_fullStr |
Genomic Regions That Underlie Soybean Seed Isoflavone Content |
title_full_unstemmed |
Genomic Regions That Underlie Soybean Seed Isoflavone Content |
title_sort |
genomic regions that underlie soybean seed isoflavone content |
publisher |
Hindawi Limited |
series |
Journal of Biomedicine and Biotechnology |
issn |
1110-7243 1110-7251 |
publishDate |
2001-01-01 |
description |
Soy products contain isoflavones (genistein, daidzein, and
glycitein) that display biological effects when ingested by
humans and animals, these effects are species, dose and age
dependent. Therefore, the content and quality of isoflavones in
soybeans is a key to their biological effect. Our objective was
to identify loci that underlie isoflavone content in soybean
seeds. The study involved 100 recombinant inbred lines (RIL) from
the cross of ‘Essex’ by ‘Forrest,’ two cultivars that contrast
for isoflavone content. Isoflavone content of seeds from each RIL
was determined by high performance liquid chromatography (HPLC).
The distribution of isoflavone content was continuous and
unimodal. The heritability estimates on a line mean basis were
79% for daidzein, 22% for genistein, and 88% for glycitein.
Isoflavone content of soybean seeds was compared against 150
polymorphic DNA markers in a one-way analysis of variance. Four
genomic regions were found to be significantly associated with
the isoflavone content of soybean seeds across both locations and
years. Molecular linkage group B1 contained a major QTL
underlying glycitein content (P=0.0001,R 2=50.2%), linkage
group N
contained a QTL for glycitein (P=0.0033,R 2=11.1%)
and a QTL for daidzein (P=0.0023,R 2=10.3%) and linkage
group A1 contained a QTL for daidzein (P=0.0081,R 2=9.6%).
Selection for these chromosomal regions in a marker assisted
selection program will allow for the manipulation of amounts and
profiles of isoflavones (genistein, daidzein, and glycitein)
content of soybean seeds. In addition, tightly linked markers can
be used in map based cloning of genes associated with
isoflavone content. |
url |
http://dx.doi.org/10.1155/S1110724301000110 |
work_keys_str_mv |
AT kmeksem genomicregionsthatunderliesoybeanseedisoflavonecontent AT vnnjiti genomicregionsthatunderliesoybeanseedisoflavonecontent AT wjbanz genomicregionsthatunderliesoybeanseedisoflavonecontent AT mjiqbal genomicregionsthatunderliesoybeanseedisoflavonecontent AT mymkassem genomicregionsthatunderliesoybeanseedisoflavonecontent AT dlhyten genomicregionsthatunderliesoybeanseedisoflavonecontent AT jyuang genomicregionsthatunderliesoybeanseedisoflavonecontent AT tawinters genomicregionsthatunderliesoybeanseedisoflavonecontent AT dalightfoot genomicregionsthatunderliesoybeanseedisoflavonecontent |
_version_ |
1724976804512399360 |