Effects of Leukocyte-Platelet-Rich Fibrin (L–PRF) on Pain, Soft Tissue Healing, Growth Factors, and Cytokines after Third Molar Extraction: A Randomized, Split-Mouth, Double-Blinded Clinical Trial
This study assessed the effects of leukocyte-platelet-rich fibrin (L–PRF) on soft tissue healing and the correlation with the local concentration of growth factors (GF) and cytokines in the dental socket of lower third molars. Forty lower-third molars (20 participants) were included in this randomiz...
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doaj-fd2c74c9c7b64a348bf9c5c58875d2df2021-02-13T00:04:15ZengMDPI AGApplied Sciences2076-34172021-02-01111666166610.3390/app11041666Effects of Leukocyte-Platelet-Rich Fibrin (L–PRF) on Pain, Soft Tissue Healing, Growth Factors, and Cytokines after Third Molar Extraction: A Randomized, Split-Mouth, Double-Blinded Clinical TrialMadelaine Torres da Silva ⊃0Carlos Fernando de Almeida Barros Mourão1Rafael Coutinho Mello-Machado2Pietro Montemezzi3Renata de Lima Barbosa4Suelen Cristina Sartoretto5Paulo Emílio Correa Leite6Kayvon Javid7Tomoyuki Kawase8Gutemberg Gomes Alves9Mônica Diuana Calasans-Maia10Graduate Program, Dentistry School, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilBiotechnology Department, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilGraduate Program, Dentistry School, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilPrivate Practice, 24128 Bergamo, ItalyBiotechnology Department, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilOral Surgery Department, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilBioengineering Program, Nacional Institute of Metrology, Quality and Technology, Duque de Caxias 25250-020, Rio de Janeiro, BrazilGraduate Program, Dentistry School, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilDivision of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, JapanBiotechnology Department, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilOral Surgery Department, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilThis study assessed the effects of leukocyte-platelet-rich fibrin (L–PRF) on soft tissue healing and the correlation with the local concentration of growth factors (GF) and cytokines in the dental socket of lower third molars. Forty lower-third molars (20 participants) were included in this randomized, double-blinded, split-mouth study. After extractions, randomized sides received alveolar filling with L–PRF on one side and a natural blood clot on the other side. The pain was assessed for up to seven days and soft tissue healing (Landry index) for 14 days post-extraction. Swabs were collected from the surgical sites for GF and cytokine assessment by flow luminometry. Participants reported lower postoperative pain on the sides grafted with L–PRF, which also presented increased tissue healing scores (<i>p </i>< 0.05). There were increased levels of all GFs and several cytokines at the L–PRF site at day one, while vascular endothelial growth factor (VEGF), IL–10, and IL1–RA remained higher throughout for seven days (<i>p </i>< 0.05). VEGF concentration at L–PRF sites correlated positively with the participants’ blood platelet content (<i>ρ</i> = 0.769). PDGF correlated negatively with pain experience on days 2 and 3, and positively with soft tissue healing scores, while FGFb presented a weak correlation with a reduction of pain on day 3. The use of L–PRF improves the soft tissue healing process and decreases postoperative pain after the third molar extractions, which correlates with an increase in the local concentration of growth factors such as PDGF and FGFb.https://www.mdpi.com/2076-3417/11/4/1666platelet-rich fibrinthird molarclinical trialgrowth factorscytokines |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Madelaine Torres da Silva ⊃ Carlos Fernando de Almeida Barros Mourão Rafael Coutinho Mello-Machado Pietro Montemezzi Renata de Lima Barbosa Suelen Cristina Sartoretto Paulo Emílio Correa Leite Kayvon Javid Tomoyuki Kawase Gutemberg Gomes Alves Mônica Diuana Calasans-Maia |
spellingShingle |
Madelaine Torres da Silva ⊃ Carlos Fernando de Almeida Barros Mourão Rafael Coutinho Mello-Machado Pietro Montemezzi Renata de Lima Barbosa Suelen Cristina Sartoretto Paulo Emílio Correa Leite Kayvon Javid Tomoyuki Kawase Gutemberg Gomes Alves Mônica Diuana Calasans-Maia Effects of Leukocyte-Platelet-Rich Fibrin (L–PRF) on Pain, Soft Tissue Healing, Growth Factors, and Cytokines after Third Molar Extraction: A Randomized, Split-Mouth, Double-Blinded Clinical Trial Applied Sciences platelet-rich fibrin third molar clinical trial growth factors cytokines |
author_facet |
Madelaine Torres da Silva ⊃ Carlos Fernando de Almeida Barros Mourão Rafael Coutinho Mello-Machado Pietro Montemezzi Renata de Lima Barbosa Suelen Cristina Sartoretto Paulo Emílio Correa Leite Kayvon Javid Tomoyuki Kawase Gutemberg Gomes Alves Mônica Diuana Calasans-Maia |
author_sort |
Madelaine Torres da Silva ⊃ |
title |
Effects of Leukocyte-Platelet-Rich Fibrin (L–PRF) on Pain, Soft Tissue Healing, Growth Factors, and Cytokines after Third Molar Extraction: A Randomized, Split-Mouth, Double-Blinded Clinical Trial |
title_short |
Effects of Leukocyte-Platelet-Rich Fibrin (L–PRF) on Pain, Soft Tissue Healing, Growth Factors, and Cytokines after Third Molar Extraction: A Randomized, Split-Mouth, Double-Blinded Clinical Trial |
title_full |
Effects of Leukocyte-Platelet-Rich Fibrin (L–PRF) on Pain, Soft Tissue Healing, Growth Factors, and Cytokines after Third Molar Extraction: A Randomized, Split-Mouth, Double-Blinded Clinical Trial |
title_fullStr |
Effects of Leukocyte-Platelet-Rich Fibrin (L–PRF) on Pain, Soft Tissue Healing, Growth Factors, and Cytokines after Third Molar Extraction: A Randomized, Split-Mouth, Double-Blinded Clinical Trial |
title_full_unstemmed |
Effects of Leukocyte-Platelet-Rich Fibrin (L–PRF) on Pain, Soft Tissue Healing, Growth Factors, and Cytokines after Third Molar Extraction: A Randomized, Split-Mouth, Double-Blinded Clinical Trial |
title_sort |
effects of leukocyte-platelet-rich fibrin (l–prf) on pain, soft tissue healing, growth factors, and cytokines after third molar extraction: a randomized, split-mouth, double-blinded clinical trial |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2021-02-01 |
description |
This study assessed the effects of leukocyte-platelet-rich fibrin (L–PRF) on soft tissue healing and the correlation with the local concentration of growth factors (GF) and cytokines in the dental socket of lower third molars. Forty lower-third molars (20 participants) were included in this randomized, double-blinded, split-mouth study. After extractions, randomized sides received alveolar filling with L–PRF on one side and a natural blood clot on the other side. The pain was assessed for up to seven days and soft tissue healing (Landry index) for 14 days post-extraction. Swabs were collected from the surgical sites for GF and cytokine assessment by flow luminometry. Participants reported lower postoperative pain on the sides grafted with L–PRF, which also presented increased tissue healing scores (<i>p </i>< 0.05). There were increased levels of all GFs and several cytokines at the L–PRF site at day one, while vascular endothelial growth factor (VEGF), IL–10, and IL1–RA remained higher throughout for seven days (<i>p </i>< 0.05). VEGF concentration at L–PRF sites correlated positively with the participants’ blood platelet content (<i>ρ</i> = 0.769). PDGF correlated negatively with pain experience on days 2 and 3, and positively with soft tissue healing scores, while FGFb presented a weak correlation with a reduction of pain on day 3. The use of L–PRF improves the soft tissue healing process and decreases postoperative pain after the third molar extractions, which correlates with an increase in the local concentration of growth factors such as PDGF and FGFb. |
topic |
platelet-rich fibrin third molar clinical trial growth factors cytokines |
url |
https://www.mdpi.com/2076-3417/11/4/1666 |
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