Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic E. coli Detection

Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic E. coli Detection

In these lab activities, students perform conventional PCR and real-time PCR to simulate pathogenic E. coli detection. The labs were designed to complement a previously published virtual PCR classroom activity in which students are asked to design a PCR-based diagnostic test for a pathogenic strain...

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Bibliographic Details
Main Author: Joanna R. Klein
Format: Article
Language:English
Published: American Society for Microbiology 2014-02-01
Series:Journal of Microbiology & Biology Education
Subjects:
E. coli
O157
H7
PCR
real-time PCR
gel electrophoresis
Online Access:http://jmbesubmissions.asm.org/index.php/jmbe/article/view/665
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spelling doaj-fd571877e1a04201ba982fc5d7597ca22020-11-25T01:00:24ZengAmerican Society for MicrobiologyJournal of Microbiology & Biology Education1935-78771935-78852014-02-0115110.1128/jmbe.v15i1.665355Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic E. coli DetectionJoanna R. Klein0University of Northwestern - St. Paul In these lab activities, students perform conventional PCR and real-time PCR to simulate pathogenic E. coli detection. The labs were designed to complement a previously published virtual PCR classroom activity in which students are asked to design a PCR-based diagnostic test for a pathogenic strain of E. coli. In the virtual PCR activity, students use bioinformatics to discover that the Shiga toxin genes (stx1 and stx2) are unique to the Enterohemorrhagic E. coli (EHEC) strain O157:H7. Thus they come to the conclusion that doing PCR with primers designed for shiga toxin should be able to differentiate O157:H7 from other strains of E. coli. In the lab activity described here, students actually perform the PCR assay. Performing PCR enhanced student understanding of the technique beyond what was accomplished through the virtual PCR classroom activity and is recommended as an addition to the case study. http://jmbesubmissions.asm.org/index.php/jmbe/article/view/665E. coliO157H7PCRreal-time PCRgel electrophoresis
collection DOAJ
language English
format Article
sources DOAJ
author Joanna R. Klein
spellingShingle Joanna R. Klein
Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic E. coli Detection
Journal of Microbiology & Biology Education
E. coli
O157
H7
PCR
real-time PCR
gel electrophoresis
author_facet Joanna R. Klein
author_sort Joanna R. Klein
title Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic E. coli Detection
title_short Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic E. coli Detection
title_full Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic E. coli Detection
title_fullStr Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic E. coli Detection
title_full_unstemmed Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic E. coli Detection
title_sort two laboratory activities using conventional or real-time pcr to simulate pathogenic e. coli detection
publisher American Society for Microbiology
series Journal of Microbiology & Biology Education
issn 1935-7877
1935-7885
publishDate 2014-02-01
description In these lab activities, students perform conventional PCR and real-time PCR to simulate pathogenic E. coli detection. The labs were designed to complement a previously published virtual PCR classroom activity in which students are asked to design a PCR-based diagnostic test for a pathogenic strain of E. coli. In the virtual PCR activity, students use bioinformatics to discover that the Shiga toxin genes (stx1 and stx2) are unique to the Enterohemorrhagic E. coli (EHEC) strain O157:H7. Thus they come to the conclusion that doing PCR with primers designed for shiga toxin should be able to differentiate O157:H7 from other strains of E. coli. In the lab activity described here, students actually perform the PCR assay. Performing PCR enhanced student understanding of the technique beyond what was accomplished through the virtual PCR classroom activity and is recommended as an addition to the case study.
topic E. coli
O157
H7
PCR
real-time PCR
gel electrophoresis
url http://jmbesubmissions.asm.org/index.php/jmbe/article/view/665
work_keys_str_mv AT joannarklein twolaboratoryactivitiesusingconventionalorrealtimepcrtosimulatepathogenicecolidetection
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