Establishment of Pluripotent Cell Cultures to Explore Allelopathic Activity of Coffee Cells by Protoplast Co-Culture Bioassay Method

• Main Authors: Shinjiro Ogita, Muchamad Imam Asrori, Hamako Sasamoto
• Format: Article
• Language: English
• Published: MDPI AG 2020-09-01
• Series: Plants
• Subjects: allelopathic activity; coffee; direct somatic embryogenesis; pluripotency; protoplast co-culture
• Online Access: https://www.mdpi.com/2223-7747/9/9/1170

 <span style="color: windowtext;">We focused on the demonstration of a new pluripotent coffee cell culture system to control the growth and metabolic functions. Somatic cells in the epidermal layer of in vitro somatic embryos (SEs) of <i>Coffea canephora</i> expressed higher pluripotency to produce secondary SEs than primary or secondary meristematic tissue. SEs were ideal explants to selectively induce functionally-differentiated cell lines, both non-embryogenic callus (nEC) and embryogenic callus (EC). The protoplast co-culture bioassay method was used to explore allelopathic activity of these cultured coffee cells. Cell wall formation of lettuce protoplasts varied after five days of co-culture. A strong stimulative reaction was observed at lower nEC protoplast densities, whereas growth was inhibited at higher densities. The reaction of lettuce protoplasts after 12 days of co-culture was recognized as an inhibitory reaction of colony formation. </span>