In Silico Screening of the Human Gut Metaproteome Identifies Th17-Promoting Peptides Encrypted in Proteins of Commensal Bacteria
Scientific studies focused on the role of the human microbiome over human health have generated billions of gigabits of genetic information during the last decade. Nowadays integration of all this information in public databases and development of pipelines allowing us to biotechnologically exploit...
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doaj-fd6fd15de9324aa7b25a07dfc3d55a142020-11-24T22:57:41ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-09-01810.3389/fmicb.2017.01726283719In Silico Screening of the Human Gut Metaproteome Identifies Th17-Promoting Peptides Encrypted in Proteins of Commensal BacteriaClaudio Hidalgo-Cantabrana0Claudio Hidalgo-Cantabrana1Marco A. Moro-García2Marco A. Moro-García3Aitor Blanco-Míguez4Aitor Blanco-Míguez5Aitor Blanco-Míguez6Florentino Fdez-Riverola7Florentino Fdez-Riverola8Anália Lourenço9Anália Lourenço10Anália Lourenço11Rebeca Alonso-Arias12Borja Sánchez13Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones CientíficasVillaviciosa, SpainDepartment of Immunology, Hospital Universitario Central de AsturiasOviedo, SpainDepartment of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones CientíficasVillaviciosa, SpainDepartment of Immunology, Hospital Universitario Central de AsturiasOviedo, SpainDepartment of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones CientíficasVillaviciosa, SpainEscuela Superior de Ingeniería Informática – Department of Computer Science, University of VigoVigo, SpainCentro de Investigaciones Biomédicas, University of VigoVigo, SpainEscuela Superior de Ingeniería Informática – Department of Computer Science, University of VigoVigo, SpainCentro de Investigaciones Biomédicas, University of VigoVigo, SpainEscuela Superior de Ingeniería Informática – Department of Computer Science, University of VigoVigo, SpainCentro de Investigaciones Biomédicas, University of VigoVigo, SpainCentre of Biological Engineering, University of MinhoBraga, PortugalDepartment of Immunology, Hospital Universitario Central de AsturiasOviedo, SpainDepartment of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones CientíficasVillaviciosa, SpainScientific studies focused on the role of the human microbiome over human health have generated billions of gigabits of genetic information during the last decade. Nowadays integration of all this information in public databases and development of pipelines allowing us to biotechnologically exploit this information are urgently needed. Prediction of the potential bioactivity of the products encoded by the human gut microbiome, or metaproteome, is the first step for identifying proteins responsible for the molecular interaction between microorganisms and the immune system. We have recently published the Mechanism of Action of the Human Microbiome (MAHMI) database (http://www.mahmi.org), conceived as a resource compiling peptide sequences with a potential immunomodulatory activity. Fifteen out of the 300 hundred million peptides contained in the MAHMI database were synthesized. These peptides were identified as being encrypted in proteins produced by gut microbiota members, they do not contain cleavage points for the major intestinal endoproteases and displayed high probability to have immunomodulatory bioactivity. The bacterial peptides FR-16 and LR-17 encrypted in proteins from Bifidobacterium longum DJ010A and Bifidobacterium fragilis YCH46 respectively, showed the higher immune modulation capability over human peripheral blood mononuclear cells. Both peptides modulated the immune response toward increases in the Th17 and decreases in the Th1 cell response, together with an induction of IL-22 production. These results strongly suggest the combined use of bioinformatics and in vitro tools as a first stage in the screening of bioactive peptides encrypted in the human gut metaproteome.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01726/fullbacterial peptidesTh17 responseCD4 cytokinesflow cytometrymicrobiomegut metaproteome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Claudio Hidalgo-Cantabrana Claudio Hidalgo-Cantabrana Marco A. Moro-García Marco A. Moro-García Aitor Blanco-Míguez Aitor Blanco-Míguez Aitor Blanco-Míguez Florentino Fdez-Riverola Florentino Fdez-Riverola Anália Lourenço Anália Lourenço Anália Lourenço Rebeca Alonso-Arias Borja Sánchez |
spellingShingle |
Claudio Hidalgo-Cantabrana Claudio Hidalgo-Cantabrana Marco A. Moro-García Marco A. Moro-García Aitor Blanco-Míguez Aitor Blanco-Míguez Aitor Blanco-Míguez Florentino Fdez-Riverola Florentino Fdez-Riverola Anália Lourenço Anália Lourenço Anália Lourenço Rebeca Alonso-Arias Borja Sánchez In Silico Screening of the Human Gut Metaproteome Identifies Th17-Promoting Peptides Encrypted in Proteins of Commensal Bacteria Frontiers in Microbiology bacterial peptides Th17 response CD4 cytokines flow cytometry microbiome gut metaproteome |
author_facet |
Claudio Hidalgo-Cantabrana Claudio Hidalgo-Cantabrana Marco A. Moro-García Marco A. Moro-García Aitor Blanco-Míguez Aitor Blanco-Míguez Aitor Blanco-Míguez Florentino Fdez-Riverola Florentino Fdez-Riverola Anália Lourenço Anália Lourenço Anália Lourenço Rebeca Alonso-Arias Borja Sánchez |
author_sort |
Claudio Hidalgo-Cantabrana |
title |
In Silico Screening of the Human Gut Metaproteome Identifies Th17-Promoting Peptides Encrypted in Proteins of Commensal Bacteria |
title_short |
In Silico Screening of the Human Gut Metaproteome Identifies Th17-Promoting Peptides Encrypted in Proteins of Commensal Bacteria |
title_full |
In Silico Screening of the Human Gut Metaproteome Identifies Th17-Promoting Peptides Encrypted in Proteins of Commensal Bacteria |
title_fullStr |
In Silico Screening of the Human Gut Metaproteome Identifies Th17-Promoting Peptides Encrypted in Proteins of Commensal Bacteria |
title_full_unstemmed |
In Silico Screening of the Human Gut Metaproteome Identifies Th17-Promoting Peptides Encrypted in Proteins of Commensal Bacteria |
title_sort |
in silico screening of the human gut metaproteome identifies th17-promoting peptides encrypted in proteins of commensal bacteria |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2017-09-01 |
description |
Scientific studies focused on the role of the human microbiome over human health have generated billions of gigabits of genetic information during the last decade. Nowadays integration of all this information in public databases and development of pipelines allowing us to biotechnologically exploit this information are urgently needed. Prediction of the potential bioactivity of the products encoded by the human gut microbiome, or metaproteome, is the first step for identifying proteins responsible for the molecular interaction between microorganisms and the immune system. We have recently published the Mechanism of Action of the Human Microbiome (MAHMI) database (http://www.mahmi.org), conceived as a resource compiling peptide sequences with a potential immunomodulatory activity. Fifteen out of the 300 hundred million peptides contained in the MAHMI database were synthesized. These peptides were identified as being encrypted in proteins produced by gut microbiota members, they do not contain cleavage points for the major intestinal endoproteases and displayed high probability to have immunomodulatory bioactivity. The bacterial peptides FR-16 and LR-17 encrypted in proteins from Bifidobacterium longum DJ010A and Bifidobacterium fragilis YCH46 respectively, showed the higher immune modulation capability over human peripheral blood mononuclear cells. Both peptides modulated the immune response toward increases in the Th17 and decreases in the Th1 cell response, together with an induction of IL-22 production. These results strongly suggest the combined use of bioinformatics and in vitro tools as a first stage in the screening of bioactive peptides encrypted in the human gut metaproteome. |
topic |
bacterial peptides Th17 response CD4 cytokines flow cytometry microbiome gut metaproteome |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.01726/full |
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