The effects of tubular structure on biomaterial aided bone regeneration in distraction osteogenesis
Summary: Background: Long duration of bone consolidation in distraction osteogenesis (DO) has been regarded as a major disadvantage. Porous bone substitutes have been long used in aiding bone formation. However the effects of geometric tubular structure of the porous scaffolds on bone formation in...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2020-11-01
|
Series: | Journal of Orthopaedic Translation |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2214031X20301200 |
id |
doaj-fd746bf4e5dd4202b097c9293d59beb0 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qi Pan Yucong Li Jia Xu Yunqing Kang Ye Li Bin Wang Yunzhi Peter Yang Sien Lin Gang Li |
spellingShingle |
Qi Pan Yucong Li Jia Xu Yunqing Kang Ye Li Bin Wang Yunzhi Peter Yang Sien Lin Gang Li The effects of tubular structure on biomaterial aided bone regeneration in distraction osteogenesis Journal of Orthopaedic Translation β-tricalcium phosphate Distraction osteogenesis Long bone defect Tubular structure |
author_facet |
Qi Pan Yucong Li Jia Xu Yunqing Kang Ye Li Bin Wang Yunzhi Peter Yang Sien Lin Gang Li |
author_sort |
Qi Pan |
title |
The effects of tubular structure on biomaterial aided bone regeneration in distraction osteogenesis |
title_short |
The effects of tubular structure on biomaterial aided bone regeneration in distraction osteogenesis |
title_full |
The effects of tubular structure on biomaterial aided bone regeneration in distraction osteogenesis |
title_fullStr |
The effects of tubular structure on biomaterial aided bone regeneration in distraction osteogenesis |
title_full_unstemmed |
The effects of tubular structure on biomaterial aided bone regeneration in distraction osteogenesis |
title_sort |
effects of tubular structure on biomaterial aided bone regeneration in distraction osteogenesis |
publisher |
Elsevier |
series |
Journal of Orthopaedic Translation |
issn |
2214-031X |
publishDate |
2020-11-01 |
description |
Summary: Background: Long duration of bone consolidation in distraction osteogenesis (DO) has been regarded as a major disadvantage. Porous bone substitutes have been long used in aiding bone formation. However the effects of geometric tubular structure of the porous scaffolds on bone formation in DO remains unknown. This study investigated the effects of porous scaffolds with three different geometric structures on bone regeneration in a rabbit DO model. Methods: β-tricalcium phosphate (β-TCP) was fabricated into no tubular, single tubular, or multi-tubular structure constructs. The microstructure of the three types of β-TCP constructs was determined by scanning electron microscope (SEM). Unilateral tibial osteotomy was made at the midshaft with 1 cm tibial shaft removed, fixed with an external lengthener in adulkt New Zealand white rabbits. After acute tibial shortening of 0.5 cm, the remaining 0.5 cm defect was implanted with β-TCP constructs with no tubular, single tubular, or multi-tubular structure. Five days after surgery, the tibia was lengthened at 0.5 mm/12 h for 5 days. After further 28 days of consolidation, the animals were terminated. Bilateral tibial specimens were collected for X-ray, micro-computed tomography examination, four-point bending mechanical test and histological analysis. Results: SEM results showed that pore sizes and pore interconnectivity in all three types of β-TCP constructs were identical. 38 days after surgery (4 weeks after lengthening), the new bone formation and mechanical properties were significantly enhanced in the groups implanted with multi-tubular and single tubular constructs, comparing to the group implanted with no tubular constructs. Furthermore, the group implanted with multi-tubular constructs had significantly better mechanical properties comparing to the group implanted with single tubular constructs. Histological results confirmed that group implanted with multi-tubular constructs had the most advanced bone formation and remodelling in the defect gap. Conclusion: The current study confirmed that β-TCP constructs with multi-tubular structure was the best choice as scaffold aid for bone regeneration in DO, indicating a novel fabrication approach of multi-tubular constructs for rapid bone formation together with DO procedure. The translational potential of this article: Porous β-TCP scaffold with a multi-tubular structure used in combination with DO procedure can significantly shorten the bone consolidation duration. The tubular shaped porous β-TCP scaffold may be used to fill bone defect and to aid bone formation, consolidaiton and remodelling in DO. |
topic |
β-tricalcium phosphate Distraction osteogenesis Long bone defect Tubular structure |
url |
http://www.sciencedirect.com/science/article/pii/S2214031X20301200 |
work_keys_str_mv |
AT qipan theeffectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT yucongli theeffectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT jiaxu theeffectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT yunqingkang theeffectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT yeli theeffectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT binwang theeffectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT yunzhipeteryang theeffectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT sienlin theeffectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT gangli theeffectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT qipan effectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT yucongli effectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT jiaxu effectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT yunqingkang effectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT yeli effectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT binwang effectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT yunzhipeteryang effectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT sienlin effectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis AT gangli effectsoftubularstructureonbiomaterialaidedboneregenerationindistractionosteogenesis |
_version_ |
1724398012546940928 |
spelling |
doaj-fd746bf4e5dd4202b097c9293d59beb02020-12-07T04:15:38ZengElsevierJournal of Orthopaedic Translation2214-031X2020-11-01258086The effects of tubular structure on biomaterial aided bone regeneration in distraction osteogenesisQi Pan0Yucong Li1Jia Xu2Yunqing Kang3Ye Li4Bin Wang5Yunzhi Peter Yang6Sien Lin7Gang Li8Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong, SAR, PR ChinaDepartment of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong, SAR, PR ChinaDepartment of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, PR ChinaDepartment of Ocean and Mechanical Engineering, Florida Atlantic University, Boca Raton, FL, USA; Department of Biomedical Science, Florida Atlantic University, Boca Raton, FL, USA; Integrative Biology Program, Department of Biological Science, Florida Atlantic University, Boca Raton, FL, USADepartment of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong, SAR, PR ChinaDepartment of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong, SAR, PR ChinaDepartment of Orthopaedic Surgery, School of Medicine, Stanford University, Stanford, CA, USA; Department of Materials Science and Engineering, School of Engineering, Stanford University, Stanford, USA; Department of Bioengineering, School of Medicine, Stanford University, Stanford, USADepartment of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong, SAR, PR China; Department of Orthopaedic Surgery, School of Medicine, Stanford University, Stanford, CA, USA; Corresponding author. Room163, 1/F, Edwards Building, Stanford University, 300 Pasteur Drive, Stanford, CA, USA.Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong, SAR, PR China; The CUHK-ACC Space Medicine Centre on Health Maintenance of Musculoskeletal System, The Chinese University of Hong Kong Shenzhen Research Institute, Shenzhen, PR China; Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, PR China; Corresponding author. Room 74038, 5F, Lui Chee Woo Clinical Science Building, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, NT, Hong Kong, SAR, PR China.Summary: Background: Long duration of bone consolidation in distraction osteogenesis (DO) has been regarded as a major disadvantage. Porous bone substitutes have been long used in aiding bone formation. However the effects of geometric tubular structure of the porous scaffolds on bone formation in DO remains unknown. This study investigated the effects of porous scaffolds with three different geometric structures on bone regeneration in a rabbit DO model. Methods: β-tricalcium phosphate (β-TCP) was fabricated into no tubular, single tubular, or multi-tubular structure constructs. The microstructure of the three types of β-TCP constructs was determined by scanning electron microscope (SEM). Unilateral tibial osteotomy was made at the midshaft with 1 cm tibial shaft removed, fixed with an external lengthener in adulkt New Zealand white rabbits. After acute tibial shortening of 0.5 cm, the remaining 0.5 cm defect was implanted with β-TCP constructs with no tubular, single tubular, or multi-tubular structure. Five days after surgery, the tibia was lengthened at 0.5 mm/12 h for 5 days. After further 28 days of consolidation, the animals were terminated. Bilateral tibial specimens were collected for X-ray, micro-computed tomography examination, four-point bending mechanical test and histological analysis. Results: SEM results showed that pore sizes and pore interconnectivity in all three types of β-TCP constructs were identical. 38 days after surgery (4 weeks after lengthening), the new bone formation and mechanical properties were significantly enhanced in the groups implanted with multi-tubular and single tubular constructs, comparing to the group implanted with no tubular constructs. Furthermore, the group implanted with multi-tubular constructs had significantly better mechanical properties comparing to the group implanted with single tubular constructs. Histological results confirmed that group implanted with multi-tubular constructs had the most advanced bone formation and remodelling in the defect gap. Conclusion: The current study confirmed that β-TCP constructs with multi-tubular structure was the best choice as scaffold aid for bone regeneration in DO, indicating a novel fabrication approach of multi-tubular constructs for rapid bone formation together with DO procedure. The translational potential of this article: Porous β-TCP scaffold with a multi-tubular structure used in combination with DO procedure can significantly shorten the bone consolidation duration. The tubular shaped porous β-TCP scaffold may be used to fill bone defect and to aid bone formation, consolidaiton and remodelling in DO.http://www.sciencedirect.com/science/article/pii/S2214031X20301200β-tricalcium phosphateDistraction osteogenesisLong bone defectTubular structure |