Elevation of C-reactive protein, P-selectin and Resistin as potential inflammatory biomarkers of urogenital Schistosomiasis exposure in preschool children
Abstract Background Schistosomiasis is known to induce inflammatory immune responses. C-reactive protein (CRP), resistin and P-selectin are serological inflammatory markers that rise during the acute stages of infection. Here, we propose such inflammatory biomarkers have a potential for use in uroge...
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doaj-fd7ea74d2b12439ab14d89052f8812932020-12-20T12:06:03ZengBMCBMC Infectious Diseases1471-23342019-12-011911810.1186/s12879-019-4690-zElevation of C-reactive protein, P-selectin and Resistin as potential inflammatory biomarkers of urogenital Schistosomiasis exposure in preschool childrenTheresa N. Chimponda0Caroline Mushayi1Derick N. M. Osakunor2Arthur Vengesai3Eyoh Enwono4Seth Amanfo5Janice Murray6Cremance Tshuma7Francisca Mutapi8Takafira Mduluza9University of Zimbabwe, Biochemistry DepartmentMashonaland Central Provincial Health Office, Ministry of Health & Child CareStatistics Department, University of ZimbabweUniversity of Zimbabwe, Biochemistry DepartmentStatistics Department, University of ZimbabweCentre for Immunity, Infection and Evolution, Institute of Immunology and Infection Research, University of Edinburgh, Ashworth LaboratoriesStatistics Department, University of ZimbabweNIHR Global Health Research Unit Tackling Infections to Benefit Africa at the University of EdinburghStatistics Department, University of ZimbabweUniversity of Zimbabwe, Biochemistry DepartmentAbstract Background Schistosomiasis is known to induce inflammatory immune responses. C-reactive protein (CRP), resistin and P-selectin are serological inflammatory markers that rise during the acute stages of infection. Here, we propose such inflammatory biomarkers have a potential for use in urogenital schistosomiasis diagnostic screening for exposure and infection in preschool-aged children. Methods As part of a larger study on urogenital schistosomiasis, 299 preschool children aged 1–5 years were included in this cross-sectional study. Parasitological diagnosis was conducted using urine filtration for Schistosoma haemtobium infection, and Kato Katz for S. mansoni infection. Serum levels of P-selectin, resistin, CRP, and antibodies against S. haematobium cercarial antigen preparation (CAP) and soluble worm antigen preparation (SWAP) were measured by ELISA. Results Of the 299 participants, 14% were egg positive for S. haematobium. Serology showed 46 and 9% of the participants to have been exposed to S. haematobium cercarial antigens and adult worm antigens, respectively. Levels of P-selectin were significantly higher in participants infected with S. haematobium (egg-positive) than in uninfected participants (p = 0.001). Levels of P-selectin were also higher in those exposed to cercarial antigen than in unexposed participants (p = 0.019). There was a positive correlation between P-selectin and infection intensity (r = 0.172; p = 0.002), as well as with IgM responses to CAP and SWAP (r = 0.183; p = 0.001); (r = 0.333; p < 0.0001) respectively. CRP significantly correlated with IgM responses to CAP (r = 0.133; p = 0.029) while resistin correlated with IgM responses to CAP and SWAP (r = 0.127; p = 0.016); (r = 0.197; p = 0.0004). CRP levels were higher in those exposed to cercarial and adult worm antigens than unexposed participants (p = 0.035); (p = 0.002) respectively, while resistin was higher in participants exposed to cercarial antigen than unexposed participants (p = 0.024). Conclusion In this preschool population, P-selectin is significantly associated with urogenital schistosome infection and intensity; hence a potential biomarker for infection diagnosis and disease monitoring. The inflammatory biomarkers (P-selectin, Resistin and CRP) were significantly higher in participants exposed to cercarial antigens than unexposed individuals indicating an underlying inflammatory environment.https://doi.org/10.1186/s12879-019-4690-zC-reactive proteinResistinP-selectinInflammatory biomarkersSchistosomiasis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Theresa N. Chimponda Caroline Mushayi Derick N. M. Osakunor Arthur Vengesai Eyoh Enwono Seth Amanfo Janice Murray Cremance Tshuma Francisca Mutapi Takafira Mduluza |
spellingShingle |
Theresa N. Chimponda Caroline Mushayi Derick N. M. Osakunor Arthur Vengesai Eyoh Enwono Seth Amanfo Janice Murray Cremance Tshuma Francisca Mutapi Takafira Mduluza Elevation of C-reactive protein, P-selectin and Resistin as potential inflammatory biomarkers of urogenital Schistosomiasis exposure in preschool children BMC Infectious Diseases C-reactive protein Resistin P-selectin Inflammatory biomarkers Schistosomiasis |
author_facet |
Theresa N. Chimponda Caroline Mushayi Derick N. M. Osakunor Arthur Vengesai Eyoh Enwono Seth Amanfo Janice Murray Cremance Tshuma Francisca Mutapi Takafira Mduluza |
author_sort |
Theresa N. Chimponda |
title |
Elevation of C-reactive protein, P-selectin and Resistin as potential inflammatory biomarkers of urogenital Schistosomiasis exposure in preschool children |
title_short |
Elevation of C-reactive protein, P-selectin and Resistin as potential inflammatory biomarkers of urogenital Schistosomiasis exposure in preschool children |
title_full |
Elevation of C-reactive protein, P-selectin and Resistin as potential inflammatory biomarkers of urogenital Schistosomiasis exposure in preschool children |
title_fullStr |
Elevation of C-reactive protein, P-selectin and Resistin as potential inflammatory biomarkers of urogenital Schistosomiasis exposure in preschool children |
title_full_unstemmed |
Elevation of C-reactive protein, P-selectin and Resistin as potential inflammatory biomarkers of urogenital Schistosomiasis exposure in preschool children |
title_sort |
elevation of c-reactive protein, p-selectin and resistin as potential inflammatory biomarkers of urogenital schistosomiasis exposure in preschool children |
publisher |
BMC |
series |
BMC Infectious Diseases |
issn |
1471-2334 |
publishDate |
2019-12-01 |
description |
Abstract Background Schistosomiasis is known to induce inflammatory immune responses. C-reactive protein (CRP), resistin and P-selectin are serological inflammatory markers that rise during the acute stages of infection. Here, we propose such inflammatory biomarkers have a potential for use in urogenital schistosomiasis diagnostic screening for exposure and infection in preschool-aged children. Methods As part of a larger study on urogenital schistosomiasis, 299 preschool children aged 1–5 years were included in this cross-sectional study. Parasitological diagnosis was conducted using urine filtration for Schistosoma haemtobium infection, and Kato Katz for S. mansoni infection. Serum levels of P-selectin, resistin, CRP, and antibodies against S. haematobium cercarial antigen preparation (CAP) and soluble worm antigen preparation (SWAP) were measured by ELISA. Results Of the 299 participants, 14% were egg positive for S. haematobium. Serology showed 46 and 9% of the participants to have been exposed to S. haematobium cercarial antigens and adult worm antigens, respectively. Levels of P-selectin were significantly higher in participants infected with S. haematobium (egg-positive) than in uninfected participants (p = 0.001). Levels of P-selectin were also higher in those exposed to cercarial antigen than in unexposed participants (p = 0.019). There was a positive correlation between P-selectin and infection intensity (r = 0.172; p = 0.002), as well as with IgM responses to CAP and SWAP (r = 0.183; p = 0.001); (r = 0.333; p < 0.0001) respectively. CRP significantly correlated with IgM responses to CAP (r = 0.133; p = 0.029) while resistin correlated with IgM responses to CAP and SWAP (r = 0.127; p = 0.016); (r = 0.197; p = 0.0004). CRP levels were higher in those exposed to cercarial and adult worm antigens than unexposed participants (p = 0.035); (p = 0.002) respectively, while resistin was higher in participants exposed to cercarial antigen than unexposed participants (p = 0.024). Conclusion In this preschool population, P-selectin is significantly associated with urogenital schistosome infection and intensity; hence a potential biomarker for infection diagnosis and disease monitoring. The inflammatory biomarkers (P-selectin, Resistin and CRP) were significantly higher in participants exposed to cercarial antigens than unexposed individuals indicating an underlying inflammatory environment. |
topic |
C-reactive protein Resistin P-selectin Inflammatory biomarkers Schistosomiasis |
url |
https://doi.org/10.1186/s12879-019-4690-z |
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