The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of <i>Aronia melanocarpa</i> juice (AMJ), high-polyphe...
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doaj-fda6828348c14bef91fffd23c4de78a72020-11-25T03:03:13ZengMDPI AGNutrients2072-66432020-05-01121484148410.3390/nu12051484The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular RiskLjiljana Stojković0Ivan Jovanović1Maja Zivković2Manja Zec3Tamara Djurić4Ivan Zivotić5Jovana Kuveljić6Ana Kolaković7Ivana Kolić8Ana Djordjević9Marija Glibetić10Dragan Alavantić11Aleksandra Stanković12Laboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaCentre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaCentre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaFoods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of <i>Aronia melanocarpa</i> juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of<i> TNF</i>, <i>IL1B</i>, <i>IL8</i>, <i>RGS1</i>, <i>OSM</i>, and <i>DUSP2</i> in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.https://www.mdpi.com/2072-6643/12/5/1484<i>Aronia melanocarpa</i>polyphenolperipheral blood mononuclear cellsmessenger RNA (mRNA)transcriptomecardiovascular risk |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ljiljana Stojković Ivan Jovanović Maja Zivković Manja Zec Tamara Djurić Ivan Zivotić Jovana Kuveljić Ana Kolaković Ivana Kolić Ana Djordjević Marija Glibetić Dragan Alavantić Aleksandra Stanković |
spellingShingle |
Ljiljana Stojković Ivan Jovanović Maja Zivković Manja Zec Tamara Djurić Ivan Zivotić Jovana Kuveljić Ana Kolaković Ivana Kolić Ana Djordjević Marija Glibetić Dragan Alavantić Aleksandra Stanković The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk Nutrients <i>Aronia melanocarpa</i> polyphenol peripheral blood mononuclear cells messenger RNA (mRNA) transcriptome cardiovascular risk |
author_facet |
Ljiljana Stojković Ivan Jovanović Maja Zivković Manja Zec Tamara Djurić Ivan Zivotić Jovana Kuveljić Ana Kolaković Ivana Kolić Ana Djordjević Marija Glibetić Dragan Alavantić Aleksandra Stanković |
author_sort |
Ljiljana Stojković |
title |
The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk |
title_short |
The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk |
title_full |
The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk |
title_fullStr |
The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk |
title_full_unstemmed |
The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk |
title_sort |
effects of <em>aronia melanocarpa</em> juice consumption on the mrna expression profile in peripheral blood mononuclear cells in subjects at cardiovascular risk |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2020-05-01 |
description |
Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of <i>Aronia melanocarpa</i> juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of<i> TNF</i>, <i>IL1B</i>, <i>IL8</i>, <i>RGS1</i>, <i>OSM</i>, and <i>DUSP2</i> in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk. |
topic |
<i>Aronia melanocarpa</i> polyphenol peripheral blood mononuclear cells messenger RNA (mRNA) transcriptome cardiovascular risk |
url |
https://www.mdpi.com/2072-6643/12/5/1484 |
work_keys_str_mv |
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