The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk

The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk

Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of <i>Aronia melanocarpa</i> juice (AMJ), high-polyphe...

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Main Authors: Ljiljana Stojković, Ivan Jovanović, Maja Zivković, Manja Zec, Tamara Djurić, Ivan Zivotić, Jovana Kuveljić, Ana Kolaković, Ivana Kolić, Ana Djordjević, Marija Glibetić, Dragan Alavantić, Aleksandra Stanković
Format: Article
Language:English
Published: MDPI AG 2020-05-01
Series:Nutrients
Subjects:
<i>Aronia melanocarpa</i>
polyphenol
peripheral blood mononuclear cells
messenger RNA (mRNA)
transcriptome
cardiovascular risk
Online Access:https://www.mdpi.com/2072-6643/12/5/1484
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spelling doaj-fda6828348c14bef91fffd23c4de78a72020-11-25T03:03:13ZengMDPI AGNutrients2072-66432020-05-01121484148410.3390/nu12051484The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular RiskLjiljana Stojković0Ivan Jovanović1Maja Zivković2Manja Zec3Tamara Djurić4Ivan Zivotić5Jovana Kuveljić6Ana Kolaković7Ivana Kolić8Ana Djordjević9Marija Glibetić10Dragan Alavantić11Aleksandra Stanković12Laboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaCentre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaCentre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Radiobiology and Molecular Genetics, “Vinča” Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, SerbiaFoods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of <i>Aronia melanocarpa</i> juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of<i> TNF</i>, <i>IL1B</i>, <i>IL8</i>, <i>RGS1</i>, <i>OSM</i>, and <i>DUSP2</i> in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.https://www.mdpi.com/2072-6643/12/5/1484<i>Aronia melanocarpa</i>polyphenolperipheral blood mononuclear cellsmessenger RNA (mRNA)transcriptomecardiovascular risk
collection DOAJ
language English
format Article
sources DOAJ
author Ljiljana Stojković
Ivan Jovanović
Maja Zivković
Manja Zec
Tamara Djurić
Ivan Zivotić
Jovana Kuveljić
Ana Kolaković
Ivana Kolić
Ana Djordjević
Marija Glibetić
Dragan Alavantić
Aleksandra Stanković
spellingShingle Ljiljana Stojković
Ivan Jovanović
Maja Zivković
Manja Zec
Tamara Djurić
Ivan Zivotić
Jovana Kuveljić
Ana Kolaković
Ivana Kolić
Ana Djordjević
Marija Glibetić
Dragan Alavantić
Aleksandra Stanković
The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
Nutrients
<i>Aronia melanocarpa</i>
polyphenol
peripheral blood mononuclear cells
messenger RNA (mRNA)
transcriptome
cardiovascular risk
author_facet Ljiljana Stojković
Ivan Jovanović
Maja Zivković
Manja Zec
Tamara Djurić
Ivan Zivotić
Jovana Kuveljić
Ana Kolaković
Ivana Kolić
Ana Djordjević
Marija Glibetić
Dragan Alavantić
Aleksandra Stanković
author_sort Ljiljana Stojković
title The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
title_short The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
title_full The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
title_fullStr The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
title_full_unstemmed The Effects of <em>Aronia melanocarpa</em> Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
title_sort effects of <em>aronia melanocarpa</em> juice consumption on the mrna expression profile in peripheral blood mononuclear cells in subjects at cardiovascular risk
publisher MDPI AG
series Nutrients
issn 2072-6643
publishDate 2020-05-01
description Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of <i>Aronia melanocarpa</i> juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of<i> TNF</i>, <i>IL1B</i>, <i>IL8</i>, <i>RGS1</i>, <i>OSM</i>, and <i>DUSP2</i> in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.
topic <i>Aronia melanocarpa</i>
polyphenol
peripheral blood mononuclear cells
messenger RNA (mRNA)
transcriptome
cardiovascular risk
url https://www.mdpi.com/2072-6643/12/5/1484
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