Integrating Deoxyribozymes into Colorimetric Sensing Platforms
Biosensors are analytical devices that have found a variety of applications in medical diagnostics, food quality control, environmental monitoring and biodefense. In recent years, functional nucleic acids, such as aptamers and nucleic acid enzymes, have shown great potential in biosensor development...
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doaj-fda9b349c7f74cc28aa1aa7af7d8333f2020-11-24T20:50:50ZengMDPI AGSensors1424-82202016-12-011612206110.3390/s16122061s16122061Integrating Deoxyribozymes into Colorimetric Sensing PlatformsDingran Chang0Sandy Zakaria1Mimi Deng2Nicholas Allen3Kha Tram4Yingfu Li5Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, CanadaDepartment of Biomedical Engineering, McMaster University, Hamilton, ON L8S 4K1, CanadaDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, CanadaDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, CanadaDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, CanadaDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, CanadaBiosensors are analytical devices that have found a variety of applications in medical diagnostics, food quality control, environmental monitoring and biodefense. In recent years, functional nucleic acids, such as aptamers and nucleic acid enzymes, have shown great potential in biosensor development due to their excellent ability in target recognition and catalysis. Deoxyribozymes (or DNAzymes) are single-stranded DNA molecules with catalytic activity and can be isolated to recognize a wide range of analytes through the process of in vitro selection. By using various signal transduction mechanisms, DNAzymes can be engineered into fluorescent, colorimetric, electrochemical and chemiluminescent biosensors. Among them, colorimetric sensors represent an attractive option as the signal can be easily detected by the naked eye. This reduces reliance on complex and expensive equipment. In this review, we will discuss the recent progress in the development of colorimetric biosensors that make use of DNAzymes and the prospect of employing these sensors in a range of chemical and biological applications.http://www.mdpi.com/1424-8220/16/12/2061DNAzymesbiosensorscolorimetric sensors |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dingran Chang Sandy Zakaria Mimi Deng Nicholas Allen Kha Tram Yingfu Li |
spellingShingle |
Dingran Chang Sandy Zakaria Mimi Deng Nicholas Allen Kha Tram Yingfu Li Integrating Deoxyribozymes into Colorimetric Sensing Platforms Sensors DNAzymes biosensors colorimetric sensors |
author_facet |
Dingran Chang Sandy Zakaria Mimi Deng Nicholas Allen Kha Tram Yingfu Li |
author_sort |
Dingran Chang |
title |
Integrating Deoxyribozymes into Colorimetric Sensing Platforms |
title_short |
Integrating Deoxyribozymes into Colorimetric Sensing Platforms |
title_full |
Integrating Deoxyribozymes into Colorimetric Sensing Platforms |
title_fullStr |
Integrating Deoxyribozymes into Colorimetric Sensing Platforms |
title_full_unstemmed |
Integrating Deoxyribozymes into Colorimetric Sensing Platforms |
title_sort |
integrating deoxyribozymes into colorimetric sensing platforms |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2016-12-01 |
description |
Biosensors are analytical devices that have found a variety of applications in medical diagnostics, food quality control, environmental monitoring and biodefense. In recent years, functional nucleic acids, such as aptamers and nucleic acid enzymes, have shown great potential in biosensor development due to their excellent ability in target recognition and catalysis. Deoxyribozymes (or DNAzymes) are single-stranded DNA molecules with catalytic activity and can be isolated to recognize a wide range of analytes through the process of in vitro selection. By using various signal transduction mechanisms, DNAzymes can be engineered into fluorescent, colorimetric, electrochemical and chemiluminescent biosensors. Among them, colorimetric sensors represent an attractive option as the signal can be easily detected by the naked eye. This reduces reliance on complex and expensive equipment. In this review, we will discuss the recent progress in the development of colorimetric biosensors that make use of DNAzymes and the prospect of employing these sensors in a range of chemical and biological applications. |
topic |
DNAzymes biosensors colorimetric sensors |
url |
http://www.mdpi.com/1424-8220/16/12/2061 |
work_keys_str_mv |
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