High Diversity and Variability in the Vaginal Microbiome in Women following Preterm Premature Rupture of Membranes (PPROM): A Prospective Cohort Study.
To characterize the vaginal microbiota of women following preterm premature rupture of membranes (PPROM), and determine if microbiome composition predicts latency duration and perinatal outcomes.A prospective cohort study.Canada.Women with PPROM between 24+0 and 33+6 weeks gestational age (GA).Micro...
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doaj-fdcbd13659a64042be70f9f458bf0d742020-11-24T22:11:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011111e016679410.1371/journal.pone.0166794High Diversity and Variability in the Vaginal Microbiome in Women following Preterm Premature Rupture of Membranes (PPROM): A Prospective Cohort Study.Teenus Paramel JayaprakashEmily C WagnerJulie van SchalkwykArianne Y K AlbertJanet E HillDeborah M MoneyPPROM Study GroupTo characterize the vaginal microbiota of women following preterm premature rupture of membranes (PPROM), and determine if microbiome composition predicts latency duration and perinatal outcomes.A prospective cohort study.Canada.Women with PPROM between 24+0 and 33+6 weeks gestational age (GA).Microbiome profiles, based on pyrosequencing of the cpn60 universal target, were generated from vaginal samples at time of presentation with PPROM, weekly thereafter, and at delivery.Vaginal microbiome composition, latency duration, gestational age at delivery, perinatal outcomes.Microbiome profiles were generated from 70 samples from 36 women. Mean GA at PPROM was 28.8 wk (mean latency 2.7 wk). Microbiome profiles were highly diverse but sequences representing Megasphaera type 1 and Prevotella spp. were detected in all vaginal samples. Only 13/70 samples were dominated by Lactobacillus spp. Microbiome profiles at the time of membrane rupture did not cluster by gestational age at PPROM, latency duration, presence of chorioamnionitis or by infant outcomes. Mycoplasma and/or Ureaplasma were detected by PCR in 81% (29/36) of women, and these women had significantly lower GA at delivery and correspondingly lower birth weight infants than Mycoplasma and/or Ureaplasma negative women.Women with PPROM had mixed, abnormal vaginal microbiota but the microbiome profile at PPROM did not correlate with latency duration. Prevotella spp. and Megasphaera type I were ubiquitous. The presence of Mollicutes in the vaginal microbiome was associated with lower GA at delivery. The microbiome was remarkably unstable during the latency period.http://europepmc.org/articles/PMC5115810?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Teenus Paramel Jayaprakash Emily C Wagner Julie van Schalkwyk Arianne Y K Albert Janet E Hill Deborah M Money PPROM Study Group |
spellingShingle |
Teenus Paramel Jayaprakash Emily C Wagner Julie van Schalkwyk Arianne Y K Albert Janet E Hill Deborah M Money PPROM Study Group High Diversity and Variability in the Vaginal Microbiome in Women following Preterm Premature Rupture of Membranes (PPROM): A Prospective Cohort Study. PLoS ONE |
author_facet |
Teenus Paramel Jayaprakash Emily C Wagner Julie van Schalkwyk Arianne Y K Albert Janet E Hill Deborah M Money PPROM Study Group |
author_sort |
Teenus Paramel Jayaprakash |
title |
High Diversity and Variability in the Vaginal Microbiome in Women following Preterm Premature Rupture of Membranes (PPROM): A Prospective Cohort Study. |
title_short |
High Diversity and Variability in the Vaginal Microbiome in Women following Preterm Premature Rupture of Membranes (PPROM): A Prospective Cohort Study. |
title_full |
High Diversity and Variability in the Vaginal Microbiome in Women following Preterm Premature Rupture of Membranes (PPROM): A Prospective Cohort Study. |
title_fullStr |
High Diversity and Variability in the Vaginal Microbiome in Women following Preterm Premature Rupture of Membranes (PPROM): A Prospective Cohort Study. |
title_full_unstemmed |
High Diversity and Variability in the Vaginal Microbiome in Women following Preterm Premature Rupture of Membranes (PPROM): A Prospective Cohort Study. |
title_sort |
high diversity and variability in the vaginal microbiome in women following preterm premature rupture of membranes (pprom): a prospective cohort study. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
To characterize the vaginal microbiota of women following preterm premature rupture of membranes (PPROM), and determine if microbiome composition predicts latency duration and perinatal outcomes.A prospective cohort study.Canada.Women with PPROM between 24+0 and 33+6 weeks gestational age (GA).Microbiome profiles, based on pyrosequencing of the cpn60 universal target, were generated from vaginal samples at time of presentation with PPROM, weekly thereafter, and at delivery.Vaginal microbiome composition, latency duration, gestational age at delivery, perinatal outcomes.Microbiome profiles were generated from 70 samples from 36 women. Mean GA at PPROM was 28.8 wk (mean latency 2.7 wk). Microbiome profiles were highly diverse but sequences representing Megasphaera type 1 and Prevotella spp. were detected in all vaginal samples. Only 13/70 samples were dominated by Lactobacillus spp. Microbiome profiles at the time of membrane rupture did not cluster by gestational age at PPROM, latency duration, presence of chorioamnionitis or by infant outcomes. Mycoplasma and/or Ureaplasma were detected by PCR in 81% (29/36) of women, and these women had significantly lower GA at delivery and correspondingly lower birth weight infants than Mycoplasma and/or Ureaplasma negative women.Women with PPROM had mixed, abnormal vaginal microbiota but the microbiome profile at PPROM did not correlate with latency duration. Prevotella spp. and Megasphaera type I were ubiquitous. The presence of Mollicutes in the vaginal microbiome was associated with lower GA at delivery. The microbiome was remarkably unstable during the latency period. |
url |
http://europepmc.org/articles/PMC5115810?pdf=render |
work_keys_str_mv |
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