A Novel ZnO-Methylene Blue Nanocomposite Matrix for Biosensing Application
A novel hybrid matrix of zinc oxide-methylene blue (ZnO-MB) has been successfully developed for biosensing application. The introduction of methylene blue into the ZnO thin film leads to reduction in the charge transfer resistance and suggests an increase in the electron transfer capacity of the com...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2011-01-01
|
Series: | International Journal of Electrochemistry |
Online Access: | http://dx.doi.org/10.4061/2011/823734 |
id |
doaj-fde422da34b14e789ddfe66c5a1f5ce1 |
---|---|
record_format |
Article |
spelling |
doaj-fde422da34b14e789ddfe66c5a1f5ce12020-11-25T00:34:20ZengHindawi LimitedInternational Journal of Electrochemistry2090-35372011-01-01201110.4061/2011/823734823734A Novel ZnO-Methylene Blue Nanocomposite Matrix for Biosensing ApplicationShibu Saha0S. K. Arya1S. P. Singh2Vinay Gupta3Department of Physics & Astrophysics, University of Delhi, Delhi 110007, IndiaBio-MEMS and Microsystem Lab, Department of Electrical Engineering, University of South Florida, Tampa, FL 33620, USADepartment of Engineering Sciences and Materials, University of Puerto Rico, Mayaguez, PR 00680, USADepartment of Physics & Astrophysics, University of Delhi, Delhi 110007, IndiaA novel hybrid matrix of zinc oxide-methylene blue (ZnO-MB) has been successfully developed for biosensing application. The introduction of methylene blue into the ZnO thin film leads to reduction in the charge transfer resistance and suggests an increase in the electron transfer capacity of the composite. Glucose oxidase (GOx) was chosen as the model enzyme and effectively immobilized on the surface of hybrid ZnO-MB nanocomposite matrix. Electrochemical measurements were employed to study biosensing response of the GOx/ZnO-MB/ITO bioelectrode as a function of glucose concentration. The low oxidation potential (−0.23 V) of the hybrid bioelectrode, in a mediatorless electrolyte, makes it resistant against interference from other bio-molecules. The low value of Michaelis-Menten constant (2.65 mM) indicates that immobilized GOx retains its enzymatic activity significantly on the surface of nanocomposite hybrid matrix that results in an enhanced affinity towards its substrate (glucose). The ZnO-MB nanocomposite hybrid matrix, exhibiting enhanced sensing response (0.2 μAmM−1cm−2) with long shelf-life (>10 weeks), has potential for the realization of an integrated biosensing device.http://dx.doi.org/10.4061/2011/823734 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shibu Saha S. K. Arya S. P. Singh Vinay Gupta |
spellingShingle |
Shibu Saha S. K. Arya S. P. Singh Vinay Gupta A Novel ZnO-Methylene Blue Nanocomposite Matrix for Biosensing Application International Journal of Electrochemistry |
author_facet |
Shibu Saha S. K. Arya S. P. Singh Vinay Gupta |
author_sort |
Shibu Saha |
title |
A Novel ZnO-Methylene Blue Nanocomposite Matrix for Biosensing Application |
title_short |
A Novel ZnO-Methylene Blue Nanocomposite Matrix for Biosensing Application |
title_full |
A Novel ZnO-Methylene Blue Nanocomposite Matrix for Biosensing Application |
title_fullStr |
A Novel ZnO-Methylene Blue Nanocomposite Matrix for Biosensing Application |
title_full_unstemmed |
A Novel ZnO-Methylene Blue Nanocomposite Matrix for Biosensing Application |
title_sort |
novel zno-methylene blue nanocomposite matrix for biosensing application |
publisher |
Hindawi Limited |
series |
International Journal of Electrochemistry |
issn |
2090-3537 |
publishDate |
2011-01-01 |
description |
A novel hybrid matrix of zinc oxide-methylene blue (ZnO-MB) has been successfully developed for biosensing application. The introduction of methylene blue into the ZnO thin film leads to reduction in the charge transfer resistance and suggests an increase in the electron transfer capacity of the composite. Glucose oxidase (GOx) was chosen as the model enzyme and effectively immobilized on the surface of hybrid ZnO-MB nanocomposite matrix. Electrochemical measurements were employed to study biosensing response of the GOx/ZnO-MB/ITO bioelectrode as a function of glucose concentration. The low oxidation potential (−0.23 V) of the hybrid bioelectrode, in a mediatorless electrolyte, makes it resistant against interference from other bio-molecules. The low value of Michaelis-Menten constant (2.65 mM) indicates that immobilized GOx retains its enzymatic activity significantly on the surface of nanocomposite hybrid matrix that results in an enhanced affinity towards its substrate (glucose). The ZnO-MB nanocomposite hybrid matrix, exhibiting enhanced sensing response (0.2 μAmM−1cm−2) with long shelf-life (>10 weeks), has potential for the realization of an integrated biosensing device. |
url |
http://dx.doi.org/10.4061/2011/823734 |
work_keys_str_mv |
AT shibusaha anovelznomethylenebluenanocompositematrixforbiosensingapplication AT skarya anovelznomethylenebluenanocompositematrixforbiosensingapplication AT spsingh anovelznomethylenebluenanocompositematrixforbiosensingapplication AT vinaygupta anovelznomethylenebluenanocompositematrixforbiosensingapplication AT shibusaha novelznomethylenebluenanocompositematrixforbiosensingapplication AT skarya novelznomethylenebluenanocompositematrixforbiosensingapplication AT spsingh novelznomethylenebluenanocompositematrixforbiosensingapplication AT vinaygupta novelznomethylenebluenanocompositematrixforbiosensingapplication |
_version_ |
1725314039669587968 |