Amino Acid Availability of a Dairy and Vegetable Protein Blend Compared to Single Casein, Whey, Soy, and Pea Proteins: A Double-Blind, Cross-Over Trial

• Main Authors: Jue Liu, Marianne Klebach, Monique Visser, Zandrie Hofman
• Format: Article
• Language: English
• Published: MDPI AG 2019-11-01
• Series: Nutrients
• Subjects: protein quality; enteral tube feed; protein blend; vegetable protein; amino acid composition; non-essential amino acid; leucine; methionine; arginine; glycine
• Online Access: https://www.mdpi.com/2072-6643/11/11/2613

Protein quality is important for patients needing medical nutrition, especially those dependent on tube feeding. A blend of dairy and vegetable proteins (35% whey, 25% casein, 20% soy, 20% pea; P4) developed to obtain a more balanced amino acid profile with higher chemical scores, was compared to its constituent single proteins. Fourteen healthy elderly subjects received P4, whey, casein, soy, and pea (18 g/360 mL bolus) on five separate visits. Blood samples were collected at baseline until 240 min after intake. Amino acid availability was calculated using incremental maximal concentration (<i>i</i>C_max) and area under the curve (<i>i</i>AUC). Availability for P4 as a sum of all amino acids was similar to casein (<i>i</i>C_max and <i>i</i>AUC) and whey (<i>i</i>C_max) and higher vs. soy (<i>i</i>C_max and <i>i</i>AUC) and pea (<i>i</i>C_max). Individual amino acid availability (<i>i</i>C_max and <i>i</i>AUC) showed different profiles reflecting the composition of the protein sources: availability of leucine and methionine was higher for P4 vs. soy and pea; availability of arginine was higher for P4 vs. casein and whey. Conclusions: The P4 amino acid profile was reflected in post-prandial plasma levels and may be regarded as more balanced compared to the constituent single proteins.