Analysis of Tetracyclines in Medicated Feed for Food Animal Production by HPLC-MS/MS
The use of medicated feed is a common practice in animal food production to improve animal health. Tetracyclines and β-Lactams are the groups that are most frequently added to this type of feed. The measurement of the concentration of the analytes in these types of samples is sometimes due to the ma...
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doaj-fdec628f9388436d997ec1d92ea8b6832020-11-24T20:41:24ZengMDPI AGAntibiotics2079-63822015-12-0151110.3390/antibiotics5010001antibiotics5010001Analysis of Tetracyclines in Medicated Feed for Food Animal Production by HPLC-MS/MSRosa Elvira Gavilán0Carolina Nebot1Jose Manuel Miranda2Yolanda Martín-Gómez3Beatriz Vázquez-Belda4Carlos Manuel Franco5Alberto Cepeda6Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, University of Santiago de Compostela, 27002 Lugo, SpainDepartment of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, University of Santiago de Compostela, 27002 Lugo, SpainDepartment of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, University of Santiago de Compostela, 27002 Lugo, SpainLaboratorio de Sanidad Animal, Lugar Barrio Jove de Arriba, 0 S/N, 33290 Gijón, SpainDepartment of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, University of Santiago de Compostela, 27002 Lugo, SpainDepartment of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, University of Santiago de Compostela, 27002 Lugo, SpainDepartment of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, University of Santiago de Compostela, 27002 Lugo, SpainThe use of medicated feed is a common practice in animal food production to improve animal health. Tetracyclines and β-Lactams are the groups that are most frequently added to this type of feed. The measurement of the concentration of the analytes in these types of samples is sometimes due to the matrix characteristic, and manufacturers are demanding fast, precise and reproducible methods. A rapid confirmatory method based on a simple extraction protocol using acidified methanol and followed by high performance liquid chromatography coupled to a tandem mass spectrometer for the quantification of four tetracyclines in feed is presented. Validation was performed following the guidelines of Decision 2002/657/EC. Results indicated that the four tetracyclines can be identified and quantified in a concentration range between 50 and 500 mg/kg with recoveries between 84% and 109% and RSD for precision under reproducible conditions between 12% and 16%. Satisfactory results were also obtained with interlaboratory studies and by comparing the method with an HPLC-Fluorescent method.http://www.mdpi.com/2079-6382/5/1/1feedantimicrobialtetracyclinesHPLC-MS/MSDecision 2002/657/EC |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rosa Elvira Gavilán Carolina Nebot Jose Manuel Miranda Yolanda Martín-Gómez Beatriz Vázquez-Belda Carlos Manuel Franco Alberto Cepeda |
spellingShingle |
Rosa Elvira Gavilán Carolina Nebot Jose Manuel Miranda Yolanda Martín-Gómez Beatriz Vázquez-Belda Carlos Manuel Franco Alberto Cepeda Analysis of Tetracyclines in Medicated Feed for Food Animal Production by HPLC-MS/MS Antibiotics feed antimicrobial tetracyclines HPLC-MS/MS Decision 2002/657/EC |
author_facet |
Rosa Elvira Gavilán Carolina Nebot Jose Manuel Miranda Yolanda Martín-Gómez Beatriz Vázquez-Belda Carlos Manuel Franco Alberto Cepeda |
author_sort |
Rosa Elvira Gavilán |
title |
Analysis of Tetracyclines in Medicated Feed for Food Animal Production by HPLC-MS/MS |
title_short |
Analysis of Tetracyclines in Medicated Feed for Food Animal Production by HPLC-MS/MS |
title_full |
Analysis of Tetracyclines in Medicated Feed for Food Animal Production by HPLC-MS/MS |
title_fullStr |
Analysis of Tetracyclines in Medicated Feed for Food Animal Production by HPLC-MS/MS |
title_full_unstemmed |
Analysis of Tetracyclines in Medicated Feed for Food Animal Production by HPLC-MS/MS |
title_sort |
analysis of tetracyclines in medicated feed for food animal production by hplc-ms/ms |
publisher |
MDPI AG |
series |
Antibiotics |
issn |
2079-6382 |
publishDate |
2015-12-01 |
description |
The use of medicated feed is a common practice in animal food production to improve animal health. Tetracyclines and β-Lactams are the groups that are most frequently added to this type of feed. The measurement of the concentration of the analytes in these types of samples is sometimes due to the matrix characteristic, and manufacturers are demanding fast, precise and reproducible methods. A rapid confirmatory method based on a simple extraction protocol using acidified methanol and followed by high performance liquid chromatography coupled to a tandem mass spectrometer for the quantification of four tetracyclines in feed is presented. Validation was performed following the guidelines of Decision 2002/657/EC. Results indicated that the four tetracyclines can be identified and quantified in a concentration range between 50 and 500 mg/kg with recoveries between 84% and 109% and RSD for precision under reproducible conditions between 12% and 16%. Satisfactory results were also obtained with interlaboratory studies and by comparing the method with an HPLC-Fluorescent method. |
topic |
feed antimicrobial tetracyclines HPLC-MS/MS Decision 2002/657/EC |
url |
http://www.mdpi.com/2079-6382/5/1/1 |
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