High expression of CDCA7 predicts poor prognosis for clear cell renal cell carcinoma and explores its associations with immunity
Abstract Background Cell division cycle-associated 7 (CDCA7), as a member of the cell division cycle associated family, was reported to be aberrantly expressed in both solid tumors and hematological tumors, suggesting its essential role in promoting tumorigenesis. Hence, we aimed to explore its comp...
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doaj-fe038abb384a424ea845085baca3e8742021-03-11T11:50:18ZengBMCCancer Cell International1475-28672021-03-0121111310.1186/s12935-021-01834-xHigh expression of CDCA7 predicts poor prognosis for clear cell renal cell carcinoma and explores its associations with immunityShouyong Liu0Yi Wang1Chenkui Miao2Qianwei Xing3Zengjun Wang4Department of Urology, The First Affiliated Hospital of Nanjing Medical UniversityDepartment of Urology, Affiliated Hospital of Nantong UniversityDepartment of Urology, The First Affiliated Hospital of Nanjing Medical UniversityDepartment of Urology, Affiliated Hospital of Nantong UniversityDepartment of Urology, The First Affiliated Hospital of Nanjing Medical UniversityAbstract Background Cell division cycle-associated 7 (CDCA7), as a member of the cell division cycle associated family, was reported to be aberrantly expressed in both solid tumors and hematological tumors, suggesting its essential role in promoting tumorigenesis. Hence, we aimed to explore its comprehensive roles of overall survival (OS) in clear cell renal cell carcinoma (ccRCC) and emphasize its associations with immunity. Methods The RNA sequencing data and corresponding clinical information were downloaded from The Cancer Genome Atlas (TCGA) database. Gene set enrichment analysis (GSEA) was adopted to explore CDCA7 associated signaling pathways. Univariate and multivariate Cox regression analyses were carried out to assess independent prognostic factors. Furthermore, roles of CDCA7 in human immunity were also investigated. Results Our results suggested that CDCA7 was overexpressed in ccRCC and its elevated expression was related to shorter OS (P < 0.01). Univariate and multivariate Cox regression analyses identified CDCA7 as an independent prognostic factor (both P < 0.05). The prognostic nomogram integrating CDCA7 expression level and clinicopathologic variables was constructed to predict 1-, 3- and 5-year OS. GSEA indicated that high CDCA7 expression was related to the apoptosis pathway, cell cycle pathway, JAK-STAT pathway, NOD like receptor pathway, P53 pathway, T cell receptor pathway and toll like receptor pathway, etc. Moreover, CDCA7 was significantly related to microsatellite instability (MSI, P < 0.001) and tumor mutational burden (TMB, P < 0.001). As for immunity, CDCA7 was remarkably associated with immune infiltration, tumor microenvironment, immune checkpoint molecules and immune pathways. Conclusions CDCA7 could serve as an independent prognostic factor for ccRCC and it was closely related to MSI, TMB, and immunity.https://doi.org/10.1186/s12935-021-01834-xCDCA7Clear cell renal cell carcinomaPrognosisSurvivalImmunity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shouyong Liu Yi Wang Chenkui Miao Qianwei Xing Zengjun Wang |
spellingShingle |
Shouyong Liu Yi Wang Chenkui Miao Qianwei Xing Zengjun Wang High expression of CDCA7 predicts poor prognosis for clear cell renal cell carcinoma and explores its associations with immunity Cancer Cell International CDCA7 Clear cell renal cell carcinoma Prognosis Survival Immunity |
author_facet |
Shouyong Liu Yi Wang Chenkui Miao Qianwei Xing Zengjun Wang |
author_sort |
Shouyong Liu |
title |
High expression of CDCA7 predicts poor prognosis for clear cell renal cell carcinoma and explores its associations with immunity |
title_short |
High expression of CDCA7 predicts poor prognosis for clear cell renal cell carcinoma and explores its associations with immunity |
title_full |
High expression of CDCA7 predicts poor prognosis for clear cell renal cell carcinoma and explores its associations with immunity |
title_fullStr |
High expression of CDCA7 predicts poor prognosis for clear cell renal cell carcinoma and explores its associations with immunity |
title_full_unstemmed |
High expression of CDCA7 predicts poor prognosis for clear cell renal cell carcinoma and explores its associations with immunity |
title_sort |
high expression of cdca7 predicts poor prognosis for clear cell renal cell carcinoma and explores its associations with immunity |
publisher |
BMC |
series |
Cancer Cell International |
issn |
1475-2867 |
publishDate |
2021-03-01 |
description |
Abstract Background Cell division cycle-associated 7 (CDCA7), as a member of the cell division cycle associated family, was reported to be aberrantly expressed in both solid tumors and hematological tumors, suggesting its essential role in promoting tumorigenesis. Hence, we aimed to explore its comprehensive roles of overall survival (OS) in clear cell renal cell carcinoma (ccRCC) and emphasize its associations with immunity. Methods The RNA sequencing data and corresponding clinical information were downloaded from The Cancer Genome Atlas (TCGA) database. Gene set enrichment analysis (GSEA) was adopted to explore CDCA7 associated signaling pathways. Univariate and multivariate Cox regression analyses were carried out to assess independent prognostic factors. Furthermore, roles of CDCA7 in human immunity were also investigated. Results Our results suggested that CDCA7 was overexpressed in ccRCC and its elevated expression was related to shorter OS (P < 0.01). Univariate and multivariate Cox regression analyses identified CDCA7 as an independent prognostic factor (both P < 0.05). The prognostic nomogram integrating CDCA7 expression level and clinicopathologic variables was constructed to predict 1-, 3- and 5-year OS. GSEA indicated that high CDCA7 expression was related to the apoptosis pathway, cell cycle pathway, JAK-STAT pathway, NOD like receptor pathway, P53 pathway, T cell receptor pathway and toll like receptor pathway, etc. Moreover, CDCA7 was significantly related to microsatellite instability (MSI, P < 0.001) and tumor mutational burden (TMB, P < 0.001). As for immunity, CDCA7 was remarkably associated with immune infiltration, tumor microenvironment, immune checkpoint molecules and immune pathways. Conclusions CDCA7 could serve as an independent prognostic factor for ccRCC and it was closely related to MSI, TMB, and immunity. |
topic |
CDCA7 Clear cell renal cell carcinoma Prognosis Survival Immunity |
url |
https://doi.org/10.1186/s12935-021-01834-x |
work_keys_str_mv |
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