Pathophysiological mechanisms of Staphylococcus non-aureus bone and joint infection: interspecies homogeneity and specific behaviour of S. pseudintermedius
Implicated in more than 60% of bone and joint infections (BJIs), Staphylococci have a particular tropism for osteoarticular tissue and lead to difficult-to-treat clinical infections. To date, Staphylococcus aureus internalization in non-professional phagocytic cells (NPPCs) is a well-explored virule...
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Frontiers Media S.A.
2016-07-01
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Series: | Frontiers in Microbiology |
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Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01063/full |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yousef Maali Yousef Maali Patrica Martins-Simoes Patrica Martins-Simoes Florent Valour Florent Valour Daniel Bouvard Daniel Bouvard Michele Bes Marisa Haenni Tristan Ferry Tristan Ferry Laurent Frederic Laurent Frederic Laurent Frederic Sophie Trouillet-Assant Sophie Trouillet-Assant |
spellingShingle |
Yousef Maali Yousef Maali Patrica Martins-Simoes Patrica Martins-Simoes Florent Valour Florent Valour Daniel Bouvard Daniel Bouvard Michele Bes Marisa Haenni Tristan Ferry Tristan Ferry Laurent Frederic Laurent Frederic Laurent Frederic Sophie Trouillet-Assant Sophie Trouillet-Assant Pathophysiological mechanisms of Staphylococcus non-aureus bone and joint infection: interspecies homogeneity and specific behaviour of S. pseudintermedius Frontiers in Microbiology invasion fibronectin Integrin α5β1 Staphylococcus pseudintermedius Staphylococcus non-aureus Bone and joint infection (BJI) |
author_facet |
Yousef Maali Yousef Maali Patrica Martins-Simoes Patrica Martins-Simoes Florent Valour Florent Valour Daniel Bouvard Daniel Bouvard Michele Bes Marisa Haenni Tristan Ferry Tristan Ferry Laurent Frederic Laurent Frederic Laurent Frederic Sophie Trouillet-Assant Sophie Trouillet-Assant |
author_sort |
Yousef Maali |
title |
Pathophysiological mechanisms of Staphylococcus non-aureus bone and joint infection: interspecies homogeneity and specific behaviour of S. pseudintermedius |
title_short |
Pathophysiological mechanisms of Staphylococcus non-aureus bone and joint infection: interspecies homogeneity and specific behaviour of S. pseudintermedius |
title_full |
Pathophysiological mechanisms of Staphylococcus non-aureus bone and joint infection: interspecies homogeneity and specific behaviour of S. pseudintermedius |
title_fullStr |
Pathophysiological mechanisms of Staphylococcus non-aureus bone and joint infection: interspecies homogeneity and specific behaviour of S. pseudintermedius |
title_full_unstemmed |
Pathophysiological mechanisms of Staphylococcus non-aureus bone and joint infection: interspecies homogeneity and specific behaviour of S. pseudintermedius |
title_sort |
pathophysiological mechanisms of staphylococcus non-aureus bone and joint infection: interspecies homogeneity and specific behaviour of s. pseudintermedius |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2016-07-01 |
description |
Implicated in more than 60% of bone and joint infections (BJIs), Staphylococci have a particular tropism for osteoarticular tissue and lead to difficult-to-treat clinical infections. To date, Staphylococcus aureus internalization in non-professional phagocytic cells (NPPCs) is a well-explored virulence mechanism involved in BJI chronicity. Conversely, the pathophysiological pathways associated with Staphylococcus non-aureus (SNA) BJIs have scarcely been studied despite their high prevalence. In this study, fifteen reference strains from 15 different SNA species were compared in terms of (i) adhesion to human fibronectin based on adhesion microplate assays and (ii) internalization ability, intracellular persistence and cytotoxicity based on an in vitro infection model using human osteoblasts. Compared to S. aureus, Staphylococcus pseudintermedius was the only species that significantly adhered to human fibronectin. This species was also associated with high (even superior to S. aureus) internalization ability, intracellular persistence and cytotoxicity. These findings were confirmed using a panel of 17 different S. pseudintermedius isolates. Additionally, S. pseudintermedius internalization by osteoblasts was completely abolished in β1 integrin-deficient murine osteoblasts. These results suggest the involvement of β1 integrin in the invasion process, although this mechanism was previously restricted to S. aureus. In summary, our results suggest that internalization into NPPCs is not a classical pathophysiologic mechanism of SNA BJIs. S. pseudintermedius appears to be an exception, and its ability to invade and subsequently induce cytotoxicity in NPPCs could explain its severe and necrotic forms of infection, notably in dogs, which exhibit a high prevalence of S. pseudintermedius infection. |
topic |
invasion fibronectin Integrin α5β1 Staphylococcus pseudintermedius Staphylococcus non-aureus Bone and joint infection (BJI) |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01063/full |
work_keys_str_mv |
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doaj-fe1ce104b4d74b70b4f27e64c5ab704f2020-11-24T20:41:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-07-01710.3389/fmicb.2016.01063208155Pathophysiological mechanisms of Staphylococcus non-aureus bone and joint infection: interspecies homogeneity and specific behaviour of S. pseudintermediusYousef Maali0Yousef Maali1Patrica Martins-Simoes2Patrica Martins-Simoes3Florent Valour4Florent Valour5Daniel Bouvard6Daniel Bouvard7Michele Bes8Marisa Haenni9Tristan Ferry10Tristan Ferry11Laurent Frederic12Laurent Frederic13Laurent Frederic14Sophie Trouillet-Assant15Sophie Trouillet-Assant16Centre International de Recherche en Infectiologie, INSERM U1111, CNRS UMR5308, Université de Lyon 1, ENS de Lyon, Team “Pathogenesis of staphylococcal infections”Department of Clinical Microbiology, Northern Hospi tal Group, Hospices Civils de Lyon,Centre International de Recherche en Infectiologie, INSERM U1111, CNRS UMR5308, Université de Lyon 1, ENS de Lyon, Team “Pathogenesis of staphylococcal infections”Department of Clinical Microbiology, Northern Hospi tal Group, Hospices Civils de Lyon,Centre International de Recherche en Infectiologie, INSERM U1111, CNRS UMR5308, Université de Lyon 1, ENS de Lyon, Team “Pathogenesis of staphylococcal infections”Infectious Diseases Department, Northern Hospital G roup, Hospices Civils de LyonAlbert Bonniot Institute, INSERM U1209Université Grenoble AlpesReference National Center of Staphylococci, Hospices Civils de LyonANSES - French Agency for Food, Environmental and Occupational Health & SafetyCentre International de Recherche en Infectiologie, INSERM U1111, CNRS UMR5308, Université de Lyon 1, ENS de Lyon, Team “Pathogenesis of staphylococcal infections”Infectious Diseases Department, Northern Hospital G roup, Hospices Civils de LyonCentre International de Recherche en Infectiologie, INSERM U1111, CNRS UMR5308, Université de Lyon 1, ENS de Lyon, Team “Pathogenesis of staphylococcal infections”Department of Clinical Microbiology, Northern Hospi tal Group, Hospices Civils de Lyon,Reference National Center of Staphylococci, Hospices Civils de LyonCentre International de Recherche en Infectiologie, INSERM U1111, CNRS UMR5308, Université de Lyon 1, ENS de Lyon, Team “Pathogenesis of staphylococcal infections”Department of Clinical Microbiology, Northern Hospi tal Group, Hospices Civils de Lyon,Implicated in more than 60% of bone and joint infections (BJIs), Staphylococci have a particular tropism for osteoarticular tissue and lead to difficult-to-treat clinical infections. To date, Staphylococcus aureus internalization in non-professional phagocytic cells (NPPCs) is a well-explored virulence mechanism involved in BJI chronicity. Conversely, the pathophysiological pathways associated with Staphylococcus non-aureus (SNA) BJIs have scarcely been studied despite their high prevalence. In this study, fifteen reference strains from 15 different SNA species were compared in terms of (i) adhesion to human fibronectin based on adhesion microplate assays and (ii) internalization ability, intracellular persistence and cytotoxicity based on an in vitro infection model using human osteoblasts. Compared to S. aureus, Staphylococcus pseudintermedius was the only species that significantly adhered to human fibronectin. This species was also associated with high (even superior to S. aureus) internalization ability, intracellular persistence and cytotoxicity. These findings were confirmed using a panel of 17 different S. pseudintermedius isolates. Additionally, S. pseudintermedius internalization by osteoblasts was completely abolished in β1 integrin-deficient murine osteoblasts. These results suggest the involvement of β1 integrin in the invasion process, although this mechanism was previously restricted to S. aureus. In summary, our results suggest that internalization into NPPCs is not a classical pathophysiologic mechanism of SNA BJIs. S. pseudintermedius appears to be an exception, and its ability to invade and subsequently induce cytotoxicity in NPPCs could explain its severe and necrotic forms of infection, notably in dogs, which exhibit a high prevalence of S. pseudintermedius infection.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01063/fullinvasionfibronectinIntegrin α5β1Staphylococcus pseudintermediusStaphylococcus non-aureusBone and joint infection (BJI) |