Selection of Salicylic Acid Tolerant Epilines in <i>Brassica napus</i>
Two of the major pathways involved in induced defense of plants against pathogens include the salicylic acid (SA)- and jasmonic acid (JA)-mediated pathways that act mainly against biotrophs and necrotrophs, respectively. However, some necrotrophic pathogens, such as <i>Botrytis cinerea</i&g...
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doaj-fe38f7650d2f477bab15bca37dbc93b72021-04-02T07:03:48ZengMDPI AGAgronomy2073-43952019-02-01929210.3390/agronomy9020092agronomy9020092Selection of Salicylic Acid Tolerant Epilines in <i>Brassica napus</i>Sonja Klemme0Yorick De Smet1Bruno P. A. Cammue2Marc De Block3BASF Agricultural Solutions Belgium NV, Technologiepark 38, 9052 Ghent, BelgiumBASF Agricultural Solutions Belgium NV, Technologiepark 38, 9052 Ghent, BelgiumCentre for Microbial & Plant Genetics, Department Microbial & Molecular Systems, University of Leuven, Kasteelpark Arenberg 20, 3001 Leuven, BelgiumBASF Agricultural Solutions Belgium NV, Technologiepark 38, 9052 Ghent, BelgiumTwo of the major pathways involved in induced defense of plants against pathogens include the salicylic acid (SA)- and jasmonic acid (JA)-mediated pathways that act mainly against biotrophs and necrotrophs, respectively. However, some necrotrophic pathogens, such as <i>Botrytis cinerea</i>, actively induce the SA pathway, resulting in cell death that allows the pathogen to proliferate in the plant. Starting from an isogenic canola (<i>Brassica napus</i>) line, epilines were selected with a reduced sensitivity to SA. The genes belonging to the SA pathway had an altered transcription profile in the SA-tolerant lines, when treated with SA. Besides the already known genes of the SA pathway, new SA target genes were identified, creating possibilities to better understand the plant defense mechanism against pathogens. The SA-tolerant line with the lowest SA-induced gene expression is tolerant to <i>Botrytis cinerea</i>. When treated with SA, this line has also a reduced histone modification (histone H3 lysine 4 trimethylation) at the genes at the start of the SA pathway.https://www.mdpi.com/2073-4395/9/2/92<i>Brassica napus</i>RNAseqChIP-Seq<i>Botrytis cinerea</i>salicylic acidepigeneticsbreedingpathogen tolerancebiotic stressascorbate |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sonja Klemme Yorick De Smet Bruno P. A. Cammue Marc De Block |
spellingShingle |
Sonja Klemme Yorick De Smet Bruno P. A. Cammue Marc De Block Selection of Salicylic Acid Tolerant Epilines in <i>Brassica napus</i> Agronomy <i>Brassica napus</i> RNAseq ChIP-Seq <i>Botrytis cinerea</i> salicylic acid epigenetics breeding pathogen tolerance biotic stress ascorbate |
author_facet |
Sonja Klemme Yorick De Smet Bruno P. A. Cammue Marc De Block |
author_sort |
Sonja Klemme |
title |
Selection of Salicylic Acid Tolerant Epilines in <i>Brassica napus</i> |
title_short |
Selection of Salicylic Acid Tolerant Epilines in <i>Brassica napus</i> |
title_full |
Selection of Salicylic Acid Tolerant Epilines in <i>Brassica napus</i> |
title_fullStr |
Selection of Salicylic Acid Tolerant Epilines in <i>Brassica napus</i> |
title_full_unstemmed |
Selection of Salicylic Acid Tolerant Epilines in <i>Brassica napus</i> |
title_sort |
selection of salicylic acid tolerant epilines in <i>brassica napus</i> |
publisher |
MDPI AG |
series |
Agronomy |
issn |
2073-4395 |
publishDate |
2019-02-01 |
description |
Two of the major pathways involved in induced defense of plants against pathogens include the salicylic acid (SA)- and jasmonic acid (JA)-mediated pathways that act mainly against biotrophs and necrotrophs, respectively. However, some necrotrophic pathogens, such as <i>Botrytis cinerea</i>, actively induce the SA pathway, resulting in cell death that allows the pathogen to proliferate in the plant. Starting from an isogenic canola (<i>Brassica napus</i>) line, epilines were selected with a reduced sensitivity to SA. The genes belonging to the SA pathway had an altered transcription profile in the SA-tolerant lines, when treated with SA. Besides the already known genes of the SA pathway, new SA target genes were identified, creating possibilities to better understand the plant defense mechanism against pathogens. The SA-tolerant line with the lowest SA-induced gene expression is tolerant to <i>Botrytis cinerea</i>. When treated with SA, this line has also a reduced histone modification (histone H3 lysine 4 trimethylation) at the genes at the start of the SA pathway. |
topic |
<i>Brassica napus</i> RNAseq ChIP-Seq <i>Botrytis cinerea</i> salicylic acid epigenetics breeding pathogen tolerance biotic stress ascorbate |
url |
https://www.mdpi.com/2073-4395/9/2/92 |
work_keys_str_mv |
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