Evaluating the association of interleukin-10 gene promoter -592 A/C polymorphism with lupus nephritis susceptibility
Background: Interleukin-10 (IL-10) is an important immunoregulatory cytokine. There are few studies evaluating the association between IL-10 and lupus nephritis (LN). The aim of this study was to evaluate the association of IL-10 gene promoter -592 A/C with LN susceptibility. Methods: The study was...
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The Korean Society of Nephrology
2016-03-01
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doaj-fe426897b012454dbde9f0a5d6a328dd2020-11-25T01:01:28ZengThe Korean Society of NephrologyKidney Research and Clinical Practice2211-91322016-03-01351293410.1016/j.krcp.2015.11.002Evaluating the association of interleukin-10 gene promoter -592 A/C polymorphism with lupus nephritis susceptibilityEmad Abdallah0Emam Waked1Mahmoud A. Abdelwahab2Department of Nephrology, Theodor Bilharz Research Institute, Cairo, EgyptDepartment of Nephrology, Theodor Bilharz Research Institute, Cairo, EgyptDepartment of Medical Biochemistry, Faculty of Medicine, Fayoum University, Faiyum, EgyptBackground: Interleukin-10 (IL-10) is an important immunoregulatory cytokine. There are few studies evaluating the association between IL-10 and lupus nephritis (LN). The aim of this study was to evaluate the association of IL-10 gene promoter -592 A/C with LN susceptibility. Methods: The study was conducted on 84 patients with systemic lupus erythematosus (SLE). Patients were divided into LN group (Group I, 48 patients) and non-LN group (Group II, 36 patients). The -592 A/C polymorphisms in IL-10 promoter gene were determined by polymerase chain reaction and restriction fragment length polymorphism in both groups. IL-10 was determined by enzyme-linked immunosorbent assay. Frequencies of the genotypes were compared between LN and non-LN patients and among LN patients with different pathologic classes. Results: There was a significant increase in serum level of IL-10 (P = 0.001) in Group I compared with Group II and significant positive correlation between serum IL-10 and SLE disease activity index (r = 0.466, P = 0.001) in Group I. There were no significant differences in the distribution of the IL-10 gene promoter -592 A/C genotypes or the allele frequencies between Groups I and II. There was no significant difference between AC/CC and AA genotypes with SLE disease activity index, proteinuria, hematuria, anti-double-stranded DNA, and IL-10 in Group I. There was no significant difference in the distribution of AC and CC genotypes among different pathologic LN classes. Conclusion: IL-10 suggested to play a role in pathogenesis and development of LN. However, the promoter -592 A/C of IL-10 gene suggested to be not associated with serum IL-10 levels or LN susceptibility. In addition, it appears that promoter -592 A/C of IL-10 gene not associated with LN activity or the pathologic classes of LN.http://www.sciencedirect.com/science/article/pii/S221191321530019XLupus nephritisPromoter -592 A/C of interleukin-10 geneSystemic lupus erythematosus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Emad Abdallah Emam Waked Mahmoud A. Abdelwahab |
spellingShingle |
Emad Abdallah Emam Waked Mahmoud A. Abdelwahab Evaluating the association of interleukin-10 gene promoter -592 A/C polymorphism with lupus nephritis susceptibility Kidney Research and Clinical Practice Lupus nephritis Promoter -592 A/C of interleukin-10 gene Systemic lupus erythematosus |
author_facet |
Emad Abdallah Emam Waked Mahmoud A. Abdelwahab |
author_sort |
Emad Abdallah |
title |
Evaluating the association of interleukin-10 gene promoter -592 A/C polymorphism with lupus nephritis susceptibility |
title_short |
Evaluating the association of interleukin-10 gene promoter -592 A/C polymorphism with lupus nephritis susceptibility |
title_full |
Evaluating the association of interleukin-10 gene promoter -592 A/C polymorphism with lupus nephritis susceptibility |
title_fullStr |
Evaluating the association of interleukin-10 gene promoter -592 A/C polymorphism with lupus nephritis susceptibility |
title_full_unstemmed |
Evaluating the association of interleukin-10 gene promoter -592 A/C polymorphism with lupus nephritis susceptibility |
title_sort |
evaluating the association of interleukin-10 gene promoter -592 a/c polymorphism with lupus nephritis susceptibility |
publisher |
The Korean Society of Nephrology |
series |
Kidney Research and Clinical Practice |
issn |
2211-9132 |
publishDate |
2016-03-01 |
description |
Background: Interleukin-10 (IL-10) is an important immunoregulatory cytokine. There are few studies evaluating the association between IL-10 and lupus nephritis (LN). The aim of this study was to evaluate the association of IL-10 gene promoter -592 A/C with LN susceptibility.
Methods: The study was conducted on 84 patients with systemic lupus erythematosus (SLE). Patients were divided into LN group (Group I, 48 patients) and non-LN group (Group II, 36 patients). The -592 A/C polymorphisms in IL-10 promoter gene were determined by polymerase chain reaction and restriction fragment length polymorphism in both groups. IL-10 was determined by enzyme-linked immunosorbent assay. Frequencies of the genotypes were compared between LN and non-LN patients and among LN patients with different pathologic classes.
Results: There was a significant increase in serum level of IL-10 (P = 0.001) in Group I compared with Group II and significant positive correlation between serum IL-10 and SLE disease activity index (r = 0.466, P = 0.001) in Group I. There were no significant differences in the distribution of the IL-10 gene promoter -592 A/C genotypes or the allele frequencies between Groups I and II. There was no significant difference between AC/CC and AA genotypes with SLE disease activity index, proteinuria, hematuria, anti-double-stranded DNA, and IL-10 in Group I. There was no significant difference in the distribution of AC and CC genotypes among different pathologic LN classes.
Conclusion: IL-10 suggested to play a role in pathogenesis and development of LN. However, the promoter -592 A/C of IL-10 gene suggested to be not associated with serum IL-10 levels or LN susceptibility. In addition, it appears that promoter -592 A/C of IL-10 gene not associated with LN activity or the pathologic classes of LN. |
topic |
Lupus nephritis Promoter -592 A/C of interleukin-10 gene Systemic lupus erythematosus |
url |
http://www.sciencedirect.com/science/article/pii/S221191321530019X |
work_keys_str_mv |
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