Dengue virus serotype infection specifies the activation of the unfolded protein response
<p>Abstract</p> <p>Background</p> <p>Dengue and Dengue hemorrhagic fever have emerged as some of the most important mosquito-borne viral diseases in the tropics. The mechanisms of pathogenesis of Dengue remain elusive. Recently, virus-induced apoptosis mediated by the U...
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doaj-fe5491d3d1484ee982f7caa8702476b32020-11-24T21:13:49ZengBMCVirology Journal1743-422X2007-09-01419110.1186/1743-422X-4-91Dengue virus serotype infection specifies the activation of the unfolded protein responseChevet EricVasudevan Subhash GWang QingPluquet OlivierUmareddy IndiraGu Feng<p>Abstract</p> <p>Background</p> <p>Dengue and Dengue hemorrhagic fever have emerged as some of the most important mosquito-borne viral diseases in the tropics. The mechanisms of pathogenesis of Dengue remain elusive. Recently, virus-induced apoptosis mediated by the Unfolded Protein Response (UPR) has been hypothesised to represent a crucial pathogenic event in viral infection. In an attempt to evaluate the contribution of the UPR to virus replication, we have characterized each component of this signalling pathway following Dengue virus infection.</p> <p>Results</p> <p>We find that upon Dengue virus infection, A549 cells elicit an UPR which is observed at the level of translation attenuation (as visualized by the phosphorylation of eIF2alpha) and activation of specific pathways such as nuclear translocation of ATF-6 and splicing of XBP-1. Interestingly, we find that specific serotype of virus modulate the UPR with different selectivity. In addition, we demonstrate that perturbation of the UPR by preventing the dephosphorylation of the translation initiation factor eIF2alpha using Salubrinal considerably alters virus infectivity.</p> <p>Conclusion</p> <p>This report provides evidence that Dengue infection induces and regulates the three branches of the UPR signaling cascades. This is a basis for our understanding of the viral regulation and conditions beneficial to the viral infection. Furthermore, modulators of UPR such as Salubrinal that inhibit Dengue replication may open up an avenue toward cell-protective agents that target the endoplasmic reticulum for anti-viral therapy.</p> http://www.virologyj.com/content/4/1/91 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chevet Eric Vasudevan Subhash G Wang Qing Pluquet Olivier Umareddy Indira Gu Feng |
spellingShingle |
Chevet Eric Vasudevan Subhash G Wang Qing Pluquet Olivier Umareddy Indira Gu Feng Dengue virus serotype infection specifies the activation of the unfolded protein response Virology Journal |
author_facet |
Chevet Eric Vasudevan Subhash G Wang Qing Pluquet Olivier Umareddy Indira Gu Feng |
author_sort |
Chevet Eric |
title |
Dengue virus serotype infection specifies the activation of the unfolded protein response |
title_short |
Dengue virus serotype infection specifies the activation of the unfolded protein response |
title_full |
Dengue virus serotype infection specifies the activation of the unfolded protein response |
title_fullStr |
Dengue virus serotype infection specifies the activation of the unfolded protein response |
title_full_unstemmed |
Dengue virus serotype infection specifies the activation of the unfolded protein response |
title_sort |
dengue virus serotype infection specifies the activation of the unfolded protein response |
publisher |
BMC |
series |
Virology Journal |
issn |
1743-422X |
publishDate |
2007-09-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Dengue and Dengue hemorrhagic fever have emerged as some of the most important mosquito-borne viral diseases in the tropics. The mechanisms of pathogenesis of Dengue remain elusive. Recently, virus-induced apoptosis mediated by the Unfolded Protein Response (UPR) has been hypothesised to represent a crucial pathogenic event in viral infection. In an attempt to evaluate the contribution of the UPR to virus replication, we have characterized each component of this signalling pathway following Dengue virus infection.</p> <p>Results</p> <p>We find that upon Dengue virus infection, A549 cells elicit an UPR which is observed at the level of translation attenuation (as visualized by the phosphorylation of eIF2alpha) and activation of specific pathways such as nuclear translocation of ATF-6 and splicing of XBP-1. Interestingly, we find that specific serotype of virus modulate the UPR with different selectivity. In addition, we demonstrate that perturbation of the UPR by preventing the dephosphorylation of the translation initiation factor eIF2alpha using Salubrinal considerably alters virus infectivity.</p> <p>Conclusion</p> <p>This report provides evidence that Dengue infection induces and regulates the three branches of the UPR signaling cascades. This is a basis for our understanding of the viral regulation and conditions beneficial to the viral infection. Furthermore, modulators of UPR such as Salubrinal that inhibit Dengue replication may open up an avenue toward cell-protective agents that target the endoplasmic reticulum for anti-viral therapy.</p> |
url |
http://www.virologyj.com/content/4/1/91 |
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1716748050035113984 |