Effects of aging on gene expression in blood of captive Tibetan macaques (Macaca thibetana) and comparisons with expression in humans
Changes in gene expression occur as animals, including primates, age. Macaques have long been used as a model species for primate evolution and biomedical studies. Here, to study gene expression in Tibetan macaques (Macaca thibetana, TMs) and its differences to humans, we applied RNA-Seq to obtain t...
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Science Press, PR China
2020-09-01
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Online Access: | http://www.zoores.ac.cn/EN/10.24272/j.issn.2095-8137.2020.092 |
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doaj-fe616f587213429896f9b69ca7d642c12020-11-25T03:49:16ZengScience Press, PR ChinaZoological Research2095-81372020-09-0141555756310.24272/j.issn.2095-8137.2020.092ZR-2020-092Effects of aging on gene expression in blood of captive Tibetan macaques (Macaca thibetana) and comparisons with expression in humansChao-Chao Yan0Xin-Shang Zhang1Liang Zhou2Qiao Yang3Min Zhou4Lin-Wan Zhang5Jin-Chuan Xing6Zhi-Feng Yan7Megan Price8Jing Li9Bi-Song Yue10Zhen-Xin Fan11Key Laboratory of Bioresources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, Sichuan 610065, ChinaInstitute of Laboratory Animal Sciences, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu, Sichuan 610212, ChinaInstitute of Laboratory Animal Sciences, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu, Sichuan 610212, ChinaKey Laboratory of Bioresources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, Sichuan 610065, ChinaKey Laboratory of Bioresources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, Sichuan 610065, ChinaKey Laboratory of Bioresources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, Sichuan 610065, ChinaDepartment of Genetics, Rutgers, State University of New Jersey, Piscataway, New Jersey 08854, USAInstitute of Laboratory Animal Sciences, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu, Sichuan 610212, ChinaKey Laboratory of Bioresources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, Sichuan 610065, ChinaKey Laboratory of Bioresources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, Sichuan 610065, ChinaKey Laboratory of Bioresources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, Sichuan 610065, ChinaKey Laboratory of Bioresources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, Sichuan 610065, ChinaChanges in gene expression occur as animals, including primates, age. Macaques have long been used as a model species for primate evolution and biomedical studies. Here, to study gene expression in Tibetan macaques (Macaca thibetana, TMs) and its differences to humans, we applied RNA-Seq to obtain the blood transcriptomes of 24 TMs. In total, 2 523 age-associated differentially expressed genes (DEGs) were identified. Several pathways and processes that regulate aging, including the FoxO signaling pathway, autophagy, and platelet activation, were significantly enriched in the up-regulated DEGs. Two significantly age-related modules were identified by weighted gene co-expression network analysis (WGCNA). The TMs and humans shared 279 common DEGs, including 111 up-regulated and 141 down-regulated genes with advancing age in the same expression direction. However, 27 age-related DEGs presented the opposite expression direction in TMs as that in humans. For example, INPPL1, with inhibitory effects on the B cell receptor signaling pathway, was up-regulated in humans but down-regulated in TMs. In general, our study suggests that aging is a critical factor affecting gene expression in the captive TM population. The similarities and differences in gene expression patterns between TMs and humans could provide new insights into primate evolution and benefit TM model development.http://www.zoores.ac.cn/EN/10.24272/j.issn.2095-8137.2020.092gene expressionagingmacaquehumantranscriptome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chao-Chao Yan Xin-Shang Zhang Liang Zhou Qiao Yang Min Zhou Lin-Wan Zhang Jin-Chuan Xing Zhi-Feng Yan Megan Price Jing Li Bi-Song Yue Zhen-Xin Fan |
spellingShingle |
Chao-Chao Yan Xin-Shang Zhang Liang Zhou Qiao Yang Min Zhou Lin-Wan Zhang Jin-Chuan Xing Zhi-Feng Yan Megan Price Jing Li Bi-Song Yue Zhen-Xin Fan Effects of aging on gene expression in blood of captive Tibetan macaques (Macaca thibetana) and comparisons with expression in humans Zoological Research gene expression aging macaque human transcriptome |
author_facet |
Chao-Chao Yan Xin-Shang Zhang Liang Zhou Qiao Yang Min Zhou Lin-Wan Zhang Jin-Chuan Xing Zhi-Feng Yan Megan Price Jing Li Bi-Song Yue Zhen-Xin Fan |
author_sort |
Chao-Chao Yan |
title |
Effects of aging on gene expression in blood of captive Tibetan macaques (Macaca thibetana) and comparisons with expression in humans |
title_short |
Effects of aging on gene expression in blood of captive Tibetan macaques (Macaca thibetana) and comparisons with expression in humans |
title_full |
Effects of aging on gene expression in blood of captive Tibetan macaques (Macaca thibetana) and comparisons with expression in humans |
title_fullStr |
Effects of aging on gene expression in blood of captive Tibetan macaques (Macaca thibetana) and comparisons with expression in humans |
title_full_unstemmed |
Effects of aging on gene expression in blood of captive Tibetan macaques (Macaca thibetana) and comparisons with expression in humans |
title_sort |
effects of aging on gene expression in blood of captive tibetan macaques (macaca thibetana) and comparisons with expression in humans |
publisher |
Science Press, PR China |
series |
Zoological Research |
issn |
2095-8137 |
publishDate |
2020-09-01 |
description |
Changes in gene expression occur as animals, including primates, age. Macaques have long been used as a model species for primate evolution and biomedical studies. Here, to study gene expression in Tibetan macaques (Macaca thibetana, TMs) and its differences to humans, we applied RNA-Seq to obtain the blood transcriptomes of 24 TMs. In total, 2 523 age-associated differentially expressed genes (DEGs) were identified. Several pathways and processes that regulate aging, including the FoxO signaling pathway, autophagy, and platelet activation, were significantly enriched in the up-regulated DEGs. Two significantly age-related modules were identified by weighted gene co-expression network analysis (WGCNA). The TMs and humans shared 279 common DEGs, including 111 up-regulated and 141 down-regulated genes with advancing age in the same expression direction. However, 27 age-related DEGs presented the opposite expression direction in TMs as that in humans. For example, INPPL1, with inhibitory effects on the B cell receptor signaling pathway, was up-regulated in humans but down-regulated in TMs. In general, our study suggests that aging is a critical factor affecting gene expression in the captive TM population. The similarities and differences in gene expression patterns between TMs and humans could provide new insights into primate evolution and benefit TM model development. |
topic |
gene expression aging macaque human transcriptome |
url |
http://www.zoores.ac.cn/EN/10.24272/j.issn.2095-8137.2020.092 |
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