In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs)
In the present study, we investigate the effects of three different Mitragyna speciosa extracts, namely methanolic, aqueous and total alkaloid extracts, on glutathione transferase-specific activity in male Sprague Dawley rat liver cytosol in vitro and in vivo. In the in vitro study, the effect of Mi...
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doaj-fe737ecee8c741f9bb2414ba874737e02020-11-24T23:47:36ZengMDPI AGMolecules1420-30492010-01-0115143244110.3390/molecules15010432In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs)Sharif Mahsufi MansorMohd Ikram Mohd SaidSurash RamanathanMohd Nizam MordiSabariah IsmailJuzaili AziziIn the present study, we investigate the effects of three different Mitragyna speciosa extracts, namely methanolic, aqueous and total alkaloid extracts, on glutathione transferase-specific activity in male Sprague Dawley rat liver cytosol in vitro and in vivo. In the in vitro study, the effect of Mitragyna speciosa extracts (0.01 to 750 µg/mL) against the specific activity of glutathione transferases was examined in rat liver cytosolic fraction from untreated rats. Our data show concentration dependent inhibition of cytosolic GSTs when Mitragyna speciosa extract was added into the reaction mixture. At the highest concentration used, the methanolic extract showed the highest GSTs specific activity inhibition (61%), followed by aqueous (50%) and total alkaloid extract (43%), respectively. In in vivo study, three different dosages; 50, 100 and 200 mg/kg for methanolic and aqueous extracts and 5, 10 and 20 mg/kg for total alkaloid extract were given orally for 14 days. An increase in GST specific activity was generally observed. However, only Mitragyna speciosa aqueous extract with a dosage of 100 mg/kg showed significant results: 129% compared to control. http://www.mdpi.com/1420-3049/15/1/432/herb-drug interactionsglutathione transferasesMitragyna speciosain vitroin vivo |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sharif Mahsufi Mansor Mohd Ikram Mohd Said Surash Ramanathan Mohd Nizam Mordi Sabariah Ismail Juzaili Azizi |
spellingShingle |
Sharif Mahsufi Mansor Mohd Ikram Mohd Said Surash Ramanathan Mohd Nizam Mordi Sabariah Ismail Juzaili Azizi In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs) Molecules herb-drug interactions glutathione transferases Mitragyna speciosa in vitro in vivo |
author_facet |
Sharif Mahsufi Mansor Mohd Ikram Mohd Said Surash Ramanathan Mohd Nizam Mordi Sabariah Ismail Juzaili Azizi |
author_sort |
Sharif Mahsufi Mansor |
title |
In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs) |
title_short |
In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs) |
title_full |
In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs) |
title_fullStr |
In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs) |
title_full_unstemmed |
In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs) |
title_sort |
in vitro and in vivo effects of three different mitragyna speciosa korth leaf extracts on phase ii drug metabolizing enzymes—glutathione transferases (gsts) |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2010-01-01 |
description |
In the present study, we investigate the effects of three different Mitragyna speciosa extracts, namely methanolic, aqueous and total alkaloid extracts, on glutathione transferase-specific activity in male Sprague Dawley rat liver cytosol in vitro and in vivo. In the in vitro study, the effect of Mitragyna speciosa extracts (0.01 to 750 µg/mL) against the specific activity of glutathione transferases was examined in rat liver cytosolic fraction from untreated rats. Our data show concentration dependent inhibition of cytosolic GSTs when Mitragyna speciosa extract was added into the reaction mixture. At the highest concentration used, the methanolic extract showed the highest GSTs specific activity inhibition (61%), followed by aqueous (50%) and total alkaloid extract (43%), respectively. In in vivo study, three different dosages; 50, 100 and 200 mg/kg for methanolic and aqueous extracts and 5, 10 and 20 mg/kg for total alkaloid extract were given orally for 14 days. An increase in GST specific activity was generally observed. However, only Mitragyna speciosa aqueous extract with a dosage of 100 mg/kg showed significant results: 129% compared to control. |
topic |
herb-drug interactions glutathione transferases Mitragyna speciosa in vitro in vivo |
url |
http://www.mdpi.com/1420-3049/15/1/432/ |
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