Dysbiosis of saliva microbiome in patients with oral lichen planus
Abstract Background Oral microbiota is not only important for maintaining oral health but also plays a role in various oral diseases. However, studies regarding microbiome changes in oral lichen planus (OLP) are very limited. To the best of our knowledge, there has been only two studies investigatin...
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doaj-fe8fab65dbd64bb2b349b9ff1e393b452020-11-25T03:23:01ZengBMCBMC Microbiology1471-21802020-04-0120111210.1186/s12866-020-01733-7Dysbiosis of saliva microbiome in patients with oral lichen planusFei Yan Yu0Qian Qian Wang1Miao Li2Ya-Hsin Cheng3Yi-Shing Lisa Cheng4Yu Zhou5Xi Yang6Fang Zhang7Xuejun Ge8Bin Zhao9Xiu Yun Ren10Department of Oral Medicine, Shanxi Medical University School and Hospital of StomatologyDepartment of Periodontology, Shanxi Medical University School and Hospital of StomatologyDepartment of Periodontology, Shanxi Medical University School and Hospital of StomatologyDepartment of Physiology, School of Medicine, China Medical UniversityDepartment of Diagnostic Sciences, Texas A & M University College of DentistryDepartment of Oral Medicine, Shanxi Medical University School and Hospital of StomatologyDepartment of Periodontology, Shanxi Medical University School and Hospital of StomatologyDepartment of Oral Medicine, Shanxi Medical University School and Hospital of StomatologyDepartment of Periodontology, Shanxi Medical University School and Hospital of StomatologyShanxi Medical University School and Hospital of StomatologyDepartment of Periodontology, Shanxi Medical University School and Hospital of StomatologyAbstract Background Oral microbiota is not only important for maintaining oral health but also plays a role in various oral diseases. However, studies regarding microbiome changes in oral lichen planus (OLP) are very limited. To the best of our knowledge, there has been only two studies investigating salivary microbiome changes in OLP. Therefore, the purpose of this study was to identify the characteristic microbial profile in the saliva of OLP patients, with or without erosive lesions, and compare that with recurrent aphthous ulcer (RAU), a common oral immunological disorder that also shows multiple erosive/ulcerative lesions. Whole saliva samples were collected from 20 patients with OLP (erosive E, n = 10 and non-erosive NE, n = 10), 10 patients with RAU (U) and 10 healthy controls (C). DNA was extracted from the saliva samples, and the 16S rDNA gene V4 hypervariable region was analyzed using Illumina sequencing. Results We obtained 4949 operational taxonomic units (OTUs) from the V4 region in all saliva samples. Community composition analysis showed a clear decreased relative abundance of genera Streptococcus and Sphingomonas in saliva from RAU patients when compared to the other three groups. Relative abundance of Lautropia and Gemella were higher in E group, whereas relative abundance of Haemophilus and Neisseria were higher in NE group when compared to C group. Abiotrophia and Oribacterium were higher in OLP (combining E and NE groups), while Eikenella and Aggregatibacter were lower when compared to C group. There was statistically significance in α-diversity between E and RAU groups(p < 0.05). Significant differences in β-diversity were detected in bacteria between E and C; NE and C; as well as E and NE groups. The LDA effect size algorithm identified the g_Haemophilus might be the potential biomarker in NE group. Conclusions We found that salivary microbiome in erosive OLP was significantly different from that found in RAU; and these changes may be related to the underlying disease process rather than presence of ulcerative/erosive lesions clinically. In addition, our findings in bacterial relative abundance in OLP were significantly different from the previously reported findings, which points to the need for further research in salivary microbiome of OLP.http://link.springer.com/article/10.1186/s12866-020-01733-7Oral lichen planusSalivary microbiome16S rDNAHigh-throughput sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fei Yan Yu Qian Qian Wang Miao Li Ya-Hsin Cheng Yi-Shing Lisa Cheng Yu Zhou Xi Yang Fang Zhang Xuejun Ge Bin Zhao Xiu Yun Ren |
spellingShingle |
Fei Yan Yu Qian Qian Wang Miao Li Ya-Hsin Cheng Yi-Shing Lisa Cheng Yu Zhou Xi Yang Fang Zhang Xuejun Ge Bin Zhao Xiu Yun Ren Dysbiosis of saliva microbiome in patients with oral lichen planus BMC Microbiology Oral lichen planus Salivary microbiome 16S rDNA High-throughput sequencing |
author_facet |
Fei Yan Yu Qian Qian Wang Miao Li Ya-Hsin Cheng Yi-Shing Lisa Cheng Yu Zhou Xi Yang Fang Zhang Xuejun Ge Bin Zhao Xiu Yun Ren |
author_sort |
Fei Yan Yu |
title |
Dysbiosis of saliva microbiome in patients with oral lichen planus |
title_short |
Dysbiosis of saliva microbiome in patients with oral lichen planus |
title_full |
Dysbiosis of saliva microbiome in patients with oral lichen planus |
title_fullStr |
Dysbiosis of saliva microbiome in patients with oral lichen planus |
title_full_unstemmed |
Dysbiosis of saliva microbiome in patients with oral lichen planus |
title_sort |
dysbiosis of saliva microbiome in patients with oral lichen planus |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2020-04-01 |
description |
Abstract Background Oral microbiota is not only important for maintaining oral health but also plays a role in various oral diseases. However, studies regarding microbiome changes in oral lichen planus (OLP) are very limited. To the best of our knowledge, there has been only two studies investigating salivary microbiome changes in OLP. Therefore, the purpose of this study was to identify the characteristic microbial profile in the saliva of OLP patients, with or without erosive lesions, and compare that with recurrent aphthous ulcer (RAU), a common oral immunological disorder that also shows multiple erosive/ulcerative lesions. Whole saliva samples were collected from 20 patients with OLP (erosive E, n = 10 and non-erosive NE, n = 10), 10 patients with RAU (U) and 10 healthy controls (C). DNA was extracted from the saliva samples, and the 16S rDNA gene V4 hypervariable region was analyzed using Illumina sequencing. Results We obtained 4949 operational taxonomic units (OTUs) from the V4 region in all saliva samples. Community composition analysis showed a clear decreased relative abundance of genera Streptococcus and Sphingomonas in saliva from RAU patients when compared to the other three groups. Relative abundance of Lautropia and Gemella were higher in E group, whereas relative abundance of Haemophilus and Neisseria were higher in NE group when compared to C group. Abiotrophia and Oribacterium were higher in OLP (combining E and NE groups), while Eikenella and Aggregatibacter were lower when compared to C group. There was statistically significance in α-diversity between E and RAU groups(p < 0.05). Significant differences in β-diversity were detected in bacteria between E and C; NE and C; as well as E and NE groups. The LDA effect size algorithm identified the g_Haemophilus might be the potential biomarker in NE group. Conclusions We found that salivary microbiome in erosive OLP was significantly different from that found in RAU; and these changes may be related to the underlying disease process rather than presence of ulcerative/erosive lesions clinically. In addition, our findings in bacterial relative abundance in OLP were significantly different from the previously reported findings, which points to the need for further research in salivary microbiome of OLP. |
topic |
Oral lichen planus Salivary microbiome 16S rDNA High-throughput sequencing |
url |
http://link.springer.com/article/10.1186/s12866-020-01733-7 |
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