Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion
(1) Background: Amikacin is an aminoglycoside antibiotic used for treating gram-negative bacterial infections in cancer patients. In this study, our aims are to investigate the migratory inhibition effects of amikacin in human MDA-MB-231 cells. (2) Methods: We used a wound-healing assay, trans-well...
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doaj-fe97a88ea40841adb22423659dd472842020-11-25T04:05:31ZengMDPI AGToxics2305-63042020-11-01810810810.3390/toxics8040108Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and InvasionYun-Hsin Wang0Yau-Hung Chen1Wen-Hao Shen2Division of Basic Research, Koo Foundation Sun Yat-Sen Cancer Center, Taipei 112, TaiwanDepartment of Chemistry, Tamkang University, Tamsui, New Taipei City 251, TaiwanDivision of Basic Research, Koo Foundation Sun Yat-Sen Cancer Center, Taipei 112, Taiwan(1) Background: Amikacin is an aminoglycoside antibiotic used for treating gram-negative bacterial infections in cancer patients. In this study, our aims are to investigate the migratory inhibition effects of amikacin in human MDA-MB-231 cells. (2) Methods: We used a wound-healing assay, trans-well analysis, Western blotting, immunostaining and siRNA knockdown approaches to investigate how amikacin influenced MDA-MB-231 cell migration and invasion. (3) Results: Wound healing showed that the MDA-MB-231 cell migration rates decreased to 44.4% in the presence of amikacin. Trans-well analysis showed that amikacin treatment led to invasion inhibition. Western blotting demonstrated that amikacin induced thioredoxin-interacting protein (TXNIP) up-regulation. TXNIP was knocked down using siRNA in MDA-MB-231 cell. Using immunostaining analysis, we found that inhibition of TXNIP expression led to MDA-MB-231 pseudopodia extension; however, amikacin treatment attenuated the cell extension formation. (4) Conclusions: We observed inhibition of migration and invasion in MDA-MB-231 cells treated with amikacin. This suggests inhibition might be mediated by up-regulation of TXNIP.https://www.mdpi.com/2305-6304/8/4/108amikacinTXNIPsiRNAbreast cancer cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yun-Hsin Wang Yau-Hung Chen Wen-Hao Shen |
spellingShingle |
Yun-Hsin Wang Yau-Hung Chen Wen-Hao Shen Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion Toxics amikacin TXNIP siRNA breast cancer cells |
author_facet |
Yun-Hsin Wang Yau-Hung Chen Wen-Hao Shen |
author_sort |
Yun-Hsin Wang |
title |
Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion |
title_short |
Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion |
title_full |
Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion |
title_fullStr |
Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion |
title_full_unstemmed |
Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion |
title_sort |
amikacin suppresses human breast cancer cell mda-mb-231 migration and invasion |
publisher |
MDPI AG |
series |
Toxics |
issn |
2305-6304 |
publishDate |
2020-11-01 |
description |
(1) Background: Amikacin is an aminoglycoside antibiotic used for treating gram-negative bacterial infections in cancer patients. In this study, our aims are to investigate the migratory inhibition effects of amikacin in human MDA-MB-231 cells. (2) Methods: We used a wound-healing assay, trans-well analysis, Western blotting, immunostaining and siRNA knockdown approaches to investigate how amikacin influenced MDA-MB-231 cell migration and invasion. (3) Results: Wound healing showed that the MDA-MB-231 cell migration rates decreased to 44.4% in the presence of amikacin. Trans-well analysis showed that amikacin treatment led to invasion inhibition. Western blotting demonstrated that amikacin induced thioredoxin-interacting protein (TXNIP) up-regulation. TXNIP was knocked down using siRNA in MDA-MB-231 cell. Using immunostaining analysis, we found that inhibition of TXNIP expression led to MDA-MB-231 pseudopodia extension; however, amikacin treatment attenuated the cell extension formation. (4) Conclusions: We observed inhibition of migration and invasion in MDA-MB-231 cells treated with amikacin. This suggests inhibition might be mediated by up-regulation of TXNIP. |
topic |
amikacin TXNIP siRNA breast cancer cells |
url |
https://www.mdpi.com/2305-6304/8/4/108 |
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