Evaluation of the microbial diversity of denitrifying bacteria in batch reactor
Microbial communities in an industrial activated sludge plant may contribute to the denitrification process, but the information on the microorganisms present in denitrifying reactors is still scarce. Removal of inorganic nitrogen compounds can be accomplished by the addition of carbon sources to th...
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doaj-fea0a6d5df15417f934d1a1d122c50392020-11-24T22:52:41ZengBrazilian Society of Chemical EngineeringBrazilian Journal of Chemical Engineering0104-66322013-09-0130345746510.1590/S0104-66322013000300004S0104-66322013000300004Evaluation of the microbial diversity of denitrifying bacteria in batch reactorS. I. Maintinguer0I. K. Sakamoto1M. A. T. Adorno2M. B. A. Varesche3Universidade Estadual PaulistaUniversidade de São PauloUniversidade de São PauloUniversidade de São PauloMicrobial communities in an industrial activated sludge plant may contribute to the denitrification process, but the information on the microorganisms present in denitrifying reactors is still scarce. Removal of inorganic nitrogen compounds can be accomplished by the addition of carbon sources to the biological process of denitrification. Ethanol is an economically viable alternative as a carbon source in tropical countries like Brazil, with large-scale production from sugarcane. This paper reports the successful aplication of activated sludge with nitrate and ethanol in a batch anaerobic reactor. The operation lasted 61.5 h with total consumption of nitrate in 42.5 h, nitrite generation (2.0 mg/L) and ethanol consumption (830.0 mg/L) in 23.5 h. Denitrifying cell counts by the most probable number at the start of the operation were lower than at the end, confirming the ability of the inoculum from activated sludge for the denitrification process. The samples from cell counts were identified as Acidovorax sp., Acinetobacter sp., Comamonas sp. and uncultured bacteria. Therefore, these species may be involved in nitrate reduction and ethanol consumption in the batch reactor.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322013000300004&lng=en&tlng=enActivated sludge systemAcidovoraxAcinetobacterNitrateEthanol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
S. I. Maintinguer I. K. Sakamoto M. A. T. Adorno M. B. A. Varesche |
spellingShingle |
S. I. Maintinguer I. K. Sakamoto M. A. T. Adorno M. B. A. Varesche Evaluation of the microbial diversity of denitrifying bacteria in batch reactor Brazilian Journal of Chemical Engineering Activated sludge system Acidovorax Acinetobacter Nitrate Ethanol |
author_facet |
S. I. Maintinguer I. K. Sakamoto M. A. T. Adorno M. B. A. Varesche |
author_sort |
S. I. Maintinguer |
title |
Evaluation of the microbial diversity of denitrifying bacteria in batch reactor |
title_short |
Evaluation of the microbial diversity of denitrifying bacteria in batch reactor |
title_full |
Evaluation of the microbial diversity of denitrifying bacteria in batch reactor |
title_fullStr |
Evaluation of the microbial diversity of denitrifying bacteria in batch reactor |
title_full_unstemmed |
Evaluation of the microbial diversity of denitrifying bacteria in batch reactor |
title_sort |
evaluation of the microbial diversity of denitrifying bacteria in batch reactor |
publisher |
Brazilian Society of Chemical Engineering |
series |
Brazilian Journal of Chemical Engineering |
issn |
0104-6632 |
publishDate |
2013-09-01 |
description |
Microbial communities in an industrial activated sludge plant may contribute to the denitrification process, but the information on the microorganisms present in denitrifying reactors is still scarce. Removal of inorganic nitrogen compounds can be accomplished by the addition of carbon sources to the biological process of denitrification. Ethanol is an economically viable alternative as a carbon source in tropical countries like Brazil, with large-scale production from sugarcane. This paper reports the successful aplication of activated sludge with nitrate and ethanol in a batch anaerobic reactor. The operation lasted 61.5 h with total consumption of nitrate in 42.5 h, nitrite generation (2.0 mg/L) and ethanol consumption (830.0 mg/L) in 23.5 h. Denitrifying cell counts by the most probable number at the start of the operation were lower than at the end, confirming the ability of the inoculum from activated sludge for the denitrification process. The samples from cell counts were identified as Acidovorax sp., Acinetobacter sp., Comamonas sp. and uncultured bacteria. Therefore, these species may be involved in nitrate reduction and ethanol consumption in the batch reactor. |
topic |
Activated sludge system Acidovorax Acinetobacter Nitrate Ethanol |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322013000300004&lng=en&tlng=en |
work_keys_str_mv |
AT simaintinguer evaluationofthemicrobialdiversityofdenitrifyingbacteriainbatchreactor AT iksakamoto evaluationofthemicrobialdiversityofdenitrifyingbacteriainbatchreactor AT matadorno evaluationofthemicrobialdiversityofdenitrifyingbacteriainbatchreactor AT mbavaresche evaluationofthemicrobialdiversityofdenitrifyingbacteriainbatchreactor |
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1725665036613976064 |