Femtosecond pump probe spectroscopy for the study of energy transfer of light-harvesting complexes from extractions of spinach leaves
Measurements of ultrafast transient processes, of temporal durations in the picosecond and femtosecond regime, are made possible by femtosecond pump probe transient absorption spectroscopy. Such an ultrafast pump probe transient absorption setup has been implemented at the CSIR National Laser Centre...
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doaj-fea3ce665e0e4cdcbc74ad8e74c97a072021-07-05T11:45:48ZengAcademy of Science of South AfricaSouth African Journal of Science1996-74892010-01-011059/10Femtosecond pump probe spectroscopy for the study of energy transfer of light-harvesting complexes from extractions of spinach leavesS. Ombinda-Lemboumba0A. du du Plessis1R.W. Sparrow2P. Molukanele3L.R. Botha4E.G. Rohwer5C.M. Steenkamp6L. van Rensburg7CSIR National Laser Centre, Laser Research Institute, Department of Physics, University of StellenboschCSIR National Laser Centre, Laser Research Institute, Department of Physics, University of StellenboschCSIR National Laser Centre, Laser Research Institute, Department of Physics, University of StellenboschCSIR National Laser Centre, cCSIR BiosciencesCSIR National Laser Centre, Laser Research Institute, Department of Physics, University of StellenboschLaser Research Institute, Department of Physics, University of StellenboschLaser Research Institute, Department of Physics, University of StellenboschdEnvironmental Science and Management, North-West UniversityMeasurements of ultrafast transient processes, of temporal durations in the picosecond and femtosecond regime, are made possible by femtosecond pump probe transient absorption spectroscopy. Such an ultrafast pump probe transient absorption setup has been implemented at the CSIR National Laser Centre and has been applied to investigate energy transfer processes in different parts of photosynthetic systems. In this paper we report on our first results obtained with Malachite green as a benchmark. Malachite green was chosen because the lifetime of its excited state is well known. We also present experimental results of the ultrafast energy transfer of light-harvesting complexes in samples prepared from spinach leaves. Various pump wavelengths in the range 600 - 680 nm were used; the probe was a white light continuum spanning 420 - 700 nm. The experimental setup is described in detail in this paper. Results obtained with these samples are consistent with those expected and achieved by other researchers in this field.http://192.168.0.108/index.php/sajs/article/view/10055 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
S. Ombinda-Lemboumba A. du du Plessis R.W. Sparrow P. Molukanele L.R. Botha E.G. Rohwer C.M. Steenkamp L. van Rensburg |
spellingShingle |
S. Ombinda-Lemboumba A. du du Plessis R.W. Sparrow P. Molukanele L.R. Botha E.G. Rohwer C.M. Steenkamp L. van Rensburg Femtosecond pump probe spectroscopy for the study of energy transfer of light-harvesting complexes from extractions of spinach leaves South African Journal of Science |
author_facet |
S. Ombinda-Lemboumba A. du du Plessis R.W. Sparrow P. Molukanele L.R. Botha E.G. Rohwer C.M. Steenkamp L. van Rensburg |
author_sort |
S. Ombinda-Lemboumba |
title |
Femtosecond pump probe spectroscopy for the study of energy transfer of light-harvesting complexes from extractions of spinach leaves |
title_short |
Femtosecond pump probe spectroscopy for the study of energy transfer of light-harvesting complexes from extractions of spinach leaves |
title_full |
Femtosecond pump probe spectroscopy for the study of energy transfer of light-harvesting complexes from extractions of spinach leaves |
title_fullStr |
Femtosecond pump probe spectroscopy for the study of energy transfer of light-harvesting complexes from extractions of spinach leaves |
title_full_unstemmed |
Femtosecond pump probe spectroscopy for the study of energy transfer of light-harvesting complexes from extractions of spinach leaves |
title_sort |
femtosecond pump probe spectroscopy for the study of energy transfer of light-harvesting complexes from extractions of spinach leaves |
publisher |
Academy of Science of South Africa |
series |
South African Journal of Science |
issn |
1996-7489 |
publishDate |
2010-01-01 |
description |
Measurements of ultrafast transient processes, of temporal durations in the picosecond and femtosecond regime, are made possible by femtosecond pump probe transient absorption spectroscopy. Such an ultrafast pump probe transient absorption setup has been implemented at the CSIR National Laser Centre and has been applied to investigate energy transfer processes in different parts of photosynthetic systems. In this paper we report on our first results obtained with Malachite green as a benchmark. Malachite green was chosen because the lifetime of its excited state is well known. We also present experimental results of the ultrafast energy transfer of light-harvesting complexes in samples prepared from spinach leaves. Various pump wavelengths in the range 600 - 680 nm were used; the probe was a white light continuum spanning 420 - 700 nm. The experimental setup is described in detail in this paper. Results obtained with these samples are consistent with those expected and achieved by other researchers in this field. |
url |
http://192.168.0.108/index.php/sajs/article/view/10055 |
work_keys_str_mv |
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