Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex
Motor coordination is supported by an array of highly organized heterogeneous modules in the cerebellum. How incoming sensorimotor information is channeled and communicated between these anatomical modules is still poorly understood. In this study, we used transgenic mice expressing GFP in specific...
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doaj-fecce531808b42f48ff88f06ee8abd0d2021-05-05T00:18:52ZengeLife Sciences Publications LtdeLife2050-084X2016-03-01510.7554/eLife.09862Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortexAntoine M Valera0Francesca Binda1Sophie A Pawlowski2Jean-Luc Dupont3Jean-François Casella4Jeffrey D Rothstein5Bernard Poulain6Philippe Isope7https://orcid.org/0000-0002-0630-5935Institut des Neurosciences Cellulaires et Intégratives, CNRS Université de Strasbourg, Strasbourg, FranceInstitut des Neurosciences Cellulaires et Intégratives, CNRS Université de Strasbourg, Strasbourg, FranceInstitut des Neurosciences Cellulaires et Intégratives, CNRS Université de Strasbourg, Strasbourg, FranceInstitut des Neurosciences Cellulaires et Intégratives, CNRS Université de Strasbourg, Strasbourg, FranceInstitut des Neurosciences Cellulaires et Intégratives, CNRS Université de Strasbourg, Strasbourg, FranceBrain Science Institute, Johns Hopkins University, School of Medicine, Baltimore, United StatesInstitut des Neurosciences Cellulaires et Intégratives, CNRS Université de Strasbourg, Strasbourg, FranceInstitut des Neurosciences Cellulaires et Intégratives, CNRS Université de Strasbourg, Strasbourg, FranceMotor coordination is supported by an array of highly organized heterogeneous modules in the cerebellum. How incoming sensorimotor information is channeled and communicated between these anatomical modules is still poorly understood. In this study, we used transgenic mice expressing GFP in specific subsets of Purkinje cells that allowed us to target a given set of cerebellar modules. Combining in vitro recordings and photostimulation, we identified stereotyped patterns of functional synaptic organization between the granule cell layer and its main targets, the Purkinje cells, Golgi cells and molecular layer interneurons. Each type of connection displayed position-specific patterns of granule cell synaptic inputs that do not strictly match with anatomical boundaries but connect distant cortical modules. Although these patterns can be adjusted by activity-dependent processes, they were found to be consistent and predictable between animals. Our results highlight the operational rules underlying communication between modules in the cerebellar cortex.https://elifesciences.org/articles/09862cerebellummodulepurkinje cellgranule cellzebrinmicrocircuit |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Antoine M Valera Francesca Binda Sophie A Pawlowski Jean-Luc Dupont Jean-François Casella Jeffrey D Rothstein Bernard Poulain Philippe Isope |
spellingShingle |
Antoine M Valera Francesca Binda Sophie A Pawlowski Jean-Luc Dupont Jean-François Casella Jeffrey D Rothstein Bernard Poulain Philippe Isope Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex eLife cerebellum module purkinje cell granule cell zebrin microcircuit |
author_facet |
Antoine M Valera Francesca Binda Sophie A Pawlowski Jean-Luc Dupont Jean-François Casella Jeffrey D Rothstein Bernard Poulain Philippe Isope |
author_sort |
Antoine M Valera |
title |
Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex |
title_short |
Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex |
title_full |
Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex |
title_fullStr |
Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex |
title_full_unstemmed |
Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex |
title_sort |
stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2016-03-01 |
description |
Motor coordination is supported by an array of highly organized heterogeneous modules in the cerebellum. How incoming sensorimotor information is channeled and communicated between these anatomical modules is still poorly understood. In this study, we used transgenic mice expressing GFP in specific subsets of Purkinje cells that allowed us to target a given set of cerebellar modules. Combining in vitro recordings and photostimulation, we identified stereotyped patterns of functional synaptic organization between the granule cell layer and its main targets, the Purkinje cells, Golgi cells and molecular layer interneurons. Each type of connection displayed position-specific patterns of granule cell synaptic inputs that do not strictly match with anatomical boundaries but connect distant cortical modules. Although these patterns can be adjusted by activity-dependent processes, they were found to be consistent and predictable between animals. Our results highlight the operational rules underlying communication between modules in the cerebellar cortex. |
topic |
cerebellum module purkinje cell granule cell zebrin microcircuit |
url |
https://elifesciences.org/articles/09862 |
work_keys_str_mv |
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1721476450504146944 |