Data on physicochemical properties of LPEI 25 kDa, PEI“Max” 40 kDa and PEIpro™

Data on physicochemical properties of LPEI 25 kDa, PEI“Max” 40 kDa and PEIpro™

The data presented in this article are related to the research article entitled “Comparative study of polyethylenimines for transient gene expression in mammalian HEK293 and CHO cells” (Delafosse et al., 2016 [1]). Polyethylenimine is a cationic polymer whose linear form has been described as the mo...

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Main Authors: Laurence Delafosse, Ping Xu, Yves Durocher
Format: Article
Language:English
Published: Elsevier 2016-09-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S235234091630350X
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spelling doaj-fed2c964ce8044689dea5d13420bb2842020-11-25T01:54:28ZengElsevierData in Brief2352-34092016-09-018456460Data on physicochemical properties of LPEI 25 kDa, PEI“Max” 40 kDa and PEIpro™Laurence Delafosse0Ping Xu1Yves Durocher2Département de biochimie et médecine moléculaire, Faculté de Médecine, Université de Montréal, QC, Canada; National Research Council of Canada, Building Montreal-Royalmount, 6100 Royalmount Avenue, Montreal, QC, Canada H4P 2R2National Research Council of Canada, Building Montreal-Royalmount, 6100 Royalmount Avenue, Montreal, QC, Canada H4P 2R2Département de biochimie et médecine moléculaire, Faculté de Médecine, Université de Montréal, QC, Canada; National Research Council of Canada, Building Montreal-Royalmount, 6100 Royalmount Avenue, Montreal, QC, Canada H4P 2R2; Corresponding author at: National Research Council of Canada, Building Montreal-Royalmount, 6100 Royalmount Avenue, Montreal, QC, Canada H4P 2R2.The data presented in this article are related to the research article entitled “Comparative study of polyethylenimines for transient gene expression in mammalian HEK293 and CHO cells” (Delafosse et al., 2016 [1]). Polyethylenimine is a cationic polymer whose linear form has been described as the most efficient to transfect a wide range of cell lines and thus is broadly used in transient gene expression. Data presented in this article compares apparent size and polydispersity as determined by size exclusion chromatography of three commercially available linear PEIs, namely LPEI, PEI“Max” and PEIpro™. Impact of those features on plasmid DNA affinity was established by plasmid DNA agarose gel migration assay.http://www.sciencedirect.com/science/article/pii/S235234091630350X
collection DOAJ
language English
format Article
sources DOAJ
author Laurence Delafosse
Ping Xu
Yves Durocher
spellingShingle Laurence Delafosse
Ping Xu
Yves Durocher
Data on physicochemical properties of LPEI 25 kDa, PEI“Max” 40 kDa and PEIpro™
Data in Brief
author_facet Laurence Delafosse
Ping Xu
Yves Durocher
author_sort Laurence Delafosse
title Data on physicochemical properties of LPEI 25 kDa, PEI“Max” 40 kDa and PEIpro™
title_short Data on physicochemical properties of LPEI 25 kDa, PEI“Max” 40 kDa and PEIpro™
title_full Data on physicochemical properties of LPEI 25 kDa, PEI“Max” 40 kDa and PEIpro™
title_fullStr Data on physicochemical properties of LPEI 25 kDa, PEI“Max” 40 kDa and PEIpro™
title_full_unstemmed Data on physicochemical properties of LPEI 25 kDa, PEI“Max” 40 kDa and PEIpro™
title_sort data on physicochemical properties of lpei 25 kda, pei“max” 40 kda and peipro™
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2016-09-01
description The data presented in this article are related to the research article entitled “Comparative study of polyethylenimines for transient gene expression in mammalian HEK293 and CHO cells” (Delafosse et al., 2016 [1]). Polyethylenimine is a cationic polymer whose linear form has been described as the most efficient to transfect a wide range of cell lines and thus is broadly used in transient gene expression. Data presented in this article compares apparent size and polydispersity as determined by size exclusion chromatography of three commercially available linear PEIs, namely LPEI, PEI“Max” and PEIpro™. Impact of those features on plasmid DNA affinity was established by plasmid DNA agarose gel migration assay.
url http://www.sciencedirect.com/science/article/pii/S235234091630350X
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AT yvesdurocher dataonphysicochemicalpropertiesoflpei25kdapeimax40kdaandpeipro
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