Simultaneous optimisation of extraction of xanthone and benzophenone α-glucosidase inhibitors from Cyclopia genistoides and identification of superior genotypes for propagation
Extraction of xanthones and benzophenones from Cyclopia genistoides was optimised in terms of solvent composition (ethanol concentration) and extraction temperature, using a central composite design, response surface methodology and multi-response desirability profiling. Compounds of interest were t...
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doaj-fed2e6a78b824b279f118ec7b7568fd62021-04-30T07:09:53ZengElsevierJournal of Functional Foods1756-46462017-06-01332131Simultaneous optimisation of extraction of xanthone and benzophenone α-glucosidase inhibitors from Cyclopia genistoides and identification of superior genotypes for propagationStephanie C. Bosman0Dalene de Beer1Theresa Beelders2Elize L. Willenburg3Christiaan J. Malherbe4Beata Walczak5Elizabeth Joubert6Department of Food Science, Stellenbosch University, Private Bag X1, Matieland 7602, South AfricaDepartment of Food Science, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa; Plant Bioactives Group, Post-Harvest and Wine Technology Division, Agricultural Research Council (ARC), Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South AfricaDepartment of Food Science, Stellenbosch University, Private Bag X1, Matieland 7602, South AfricaPlant Bioactives Group, Post-Harvest and Wine Technology Division, Agricultural Research Council (ARC), Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South AfricaPlant Bioactives Group, Post-Harvest and Wine Technology Division, Agricultural Research Council (ARC), Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South AfricaInstitute of Chemistry, The University of Silesia, 9 Szkolna Street, 40-006 Katowice, PolandDepartment of Food Science, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa; Plant Bioactives Group, Post-Harvest and Wine Technology Division, Agricultural Research Council (ARC), Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South Africa; Corresponding author at: Plant Bioactives Group, Post-Harvest and Wine Technology Division, Agricultural Research Council (ARC), Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South Africa.Extraction of xanthones and benzophenones from Cyclopia genistoides was optimised in terms of solvent composition (ethanol concentration) and extraction temperature, using a central composite design, response surface methodology and multi-response desirability profiling. Compounds of interest were the xanthones, mangiferin and isomangiferin, and the benzophenones, 3-β-d-glucopyranosyl-4-β-d-glucopyranosyloxyiriflophenone and 3-β-d-glucopyranosyliriflophenone, present in high levels in C. genistoides. Optimum extraction conditions (40% ethanol; 70 °C; 10:1 solvent:solid ratio; 30 min) applied to different batches of plant material (n = 10), delivered mean extract, xanthone and benzophenone yields of 37.8, 4.12 and 1.99 g/100 g plant material, respectively, with a mean α-glucosidase inhibitory activity of 39.5% at 100 μg/mL. Similar α-glucosidase inhibitory activity was demonstrated for mangiferin and isomangiferin, indicating that the position of the sugar moiety on the dibenzo-γ-pirone structure did not affect activity. Superior C. genistoides genotypes in terms of xanthone and benzophenone content were identified.http://www.sciencedirect.com/science/article/pii/S1756464617301287Mammalian α-glucosidase inhibitionNutraceutical extractPhenolic contentPlant selectionResponse surface methodology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stephanie C. Bosman Dalene de Beer Theresa Beelders Elize L. Willenburg Christiaan J. Malherbe Beata Walczak Elizabeth Joubert |
spellingShingle |
Stephanie C. Bosman Dalene de Beer Theresa Beelders Elize L. Willenburg Christiaan J. Malherbe Beata Walczak Elizabeth Joubert Simultaneous optimisation of extraction of xanthone and benzophenone α-glucosidase inhibitors from Cyclopia genistoides and identification of superior genotypes for propagation Journal of Functional Foods Mammalian α-glucosidase inhibition Nutraceutical extract Phenolic content Plant selection Response surface methodology |
author_facet |
Stephanie C. Bosman Dalene de Beer Theresa Beelders Elize L. Willenburg Christiaan J. Malherbe Beata Walczak Elizabeth Joubert |
author_sort |
Stephanie C. Bosman |
title |
Simultaneous optimisation of extraction of xanthone and benzophenone α-glucosidase inhibitors from Cyclopia genistoides and identification of superior genotypes for propagation |
title_short |
Simultaneous optimisation of extraction of xanthone and benzophenone α-glucosidase inhibitors from Cyclopia genistoides and identification of superior genotypes for propagation |
title_full |
Simultaneous optimisation of extraction of xanthone and benzophenone α-glucosidase inhibitors from Cyclopia genistoides and identification of superior genotypes for propagation |
title_fullStr |
Simultaneous optimisation of extraction of xanthone and benzophenone α-glucosidase inhibitors from Cyclopia genistoides and identification of superior genotypes for propagation |
title_full_unstemmed |
Simultaneous optimisation of extraction of xanthone and benzophenone α-glucosidase inhibitors from Cyclopia genistoides and identification of superior genotypes for propagation |
title_sort |
simultaneous optimisation of extraction of xanthone and benzophenone α-glucosidase inhibitors from cyclopia genistoides and identification of superior genotypes for propagation |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2017-06-01 |
description |
Extraction of xanthones and benzophenones from Cyclopia genistoides was optimised in terms of solvent composition (ethanol concentration) and extraction temperature, using a central composite design, response surface methodology and multi-response desirability profiling. Compounds of interest were the xanthones, mangiferin and isomangiferin, and the benzophenones, 3-β-d-glucopyranosyl-4-β-d-glucopyranosyloxyiriflophenone and 3-β-d-glucopyranosyliriflophenone, present in high levels in C. genistoides. Optimum extraction conditions (40% ethanol; 70 °C; 10:1 solvent:solid ratio; 30 min) applied to different batches of plant material (n = 10), delivered mean extract, xanthone and benzophenone yields of 37.8, 4.12 and 1.99 g/100 g plant material, respectively, with a mean α-glucosidase inhibitory activity of 39.5% at 100 μg/mL. Similar α-glucosidase inhibitory activity was demonstrated for mangiferin and isomangiferin, indicating that the position of the sugar moiety on the dibenzo-γ-pirone structure did not affect activity. Superior C. genistoides genotypes in terms of xanthone and benzophenone content were identified. |
topic |
Mammalian α-glucosidase inhibition Nutraceutical extract Phenolic content Plant selection Response surface methodology |
url |
http://www.sciencedirect.com/science/article/pii/S1756464617301287 |
work_keys_str_mv |
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