Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1

Changes in influenza viruses require regular reformulation of strain-specific influenza vaccines. Vaccines based on conserved antigens provide broader protection. Influenza matrix protein 2 (M2) is highly conserved across influenza A subtypes. To evaluate its efficacy as a vaccine candidate, we vacc...

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Main Authors: Stephen Mark Tompkins, Zi-Shan Zhao, Chia-Yun Lo, Julia A. Misplon, Teresa Liu, Zhiping Ye, Robert J. Hogan, Zhengqi Wu, Kimberly A. Benton, Terrence M. Tumpey, Suzanne L. Epstein
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2007-03-01
Series:Emerging Infectious Diseases
Subjects:
Online Access:https://wwwnc.cdc.gov/eid/article/13/3/06-1125_article
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spelling doaj-fee9914cef934acbac16c51803f85cd72020-11-25T01:04:37ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592007-03-0113342642610.3201/eid1303.061125Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1Stephen Mark TompkinsZi-Shan ZhaoChia-Yun LoJulia A. MisplonTeresa LiuZhiping YeRobert J. HoganZhengqi WuKimberly A. BentonTerrence M. TumpeySuzanne L. EpsteinChanges in influenza viruses require regular reformulation of strain-specific influenza vaccines. Vaccines based on conserved antigens provide broader protection. Influenza matrix protein 2 (M2) is highly conserved across influenza A subtypes. To evaluate its efficacy as a vaccine candidate, we vaccinated mice with M2 peptide of a widely shared consensus sequence. This vaccination induced antibodies that cross-reacted with divergent M2 peptide from an H5N1 subtype. A DNA vaccine expressing full-length consensus-sequence M2 (M2-DNA) induced M2-specific antibody responses and protected against challenge with lethal influenza. Mice primed with M2-DNA and then boosted with recombinant adenovirus expressing M2 (M2-Ad) had enhanced antibody responses that cross-reacted with human and avian M2 sequences and induced T-cell responses. This M2 prime-boost vaccination conferred broad protection against challenge with lethal influenza A, including an H5N1 strain. Vaccination with M2, with key sequences represented, may provide broad protection against influenza A.https://wwwnc.cdc.gov/eid/article/13/3/06-1125_articleDNA vaccinesM2 proteininfluenza A virus H1N1 subtypeinfluenza A virus H3N2 subtypeinfluenza A virus H5N1 subtyperesearch
collection DOAJ
language English
format Article
sources DOAJ
author Stephen Mark Tompkins
Zi-Shan Zhao
Chia-Yun Lo
Julia A. Misplon
Teresa Liu
Zhiping Ye
Robert J. Hogan
Zhengqi Wu
Kimberly A. Benton
Terrence M. Tumpey
Suzanne L. Epstein
spellingShingle Stephen Mark Tompkins
Zi-Shan Zhao
Chia-Yun Lo
Julia A. Misplon
Teresa Liu
Zhiping Ye
Robert J. Hogan
Zhengqi Wu
Kimberly A. Benton
Terrence M. Tumpey
Suzanne L. Epstein
Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1
Emerging Infectious Diseases
DNA vaccines
M2 protein
influenza A virus H1N1 subtype
influenza A virus H3N2 subtype
influenza A virus H5N1 subtype
research
author_facet Stephen Mark Tompkins
Zi-Shan Zhao
Chia-Yun Lo
Julia A. Misplon
Teresa Liu
Zhiping Ye
Robert J. Hogan
Zhengqi Wu
Kimberly A. Benton
Terrence M. Tumpey
Suzanne L. Epstein
author_sort Stephen Mark Tompkins
title Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1
title_short Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1
title_full Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1
title_fullStr Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1
title_full_unstemmed Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1
title_sort matrix protein 2 vaccination and protection against influenza viruses, including subtype h5n1
publisher Centers for Disease Control and Prevention
series Emerging Infectious Diseases
issn 1080-6040
1080-6059
publishDate 2007-03-01
description Changes in influenza viruses require regular reformulation of strain-specific influenza vaccines. Vaccines based on conserved antigens provide broader protection. Influenza matrix protein 2 (M2) is highly conserved across influenza A subtypes. To evaluate its efficacy as a vaccine candidate, we vaccinated mice with M2 peptide of a widely shared consensus sequence. This vaccination induced antibodies that cross-reacted with divergent M2 peptide from an H5N1 subtype. A DNA vaccine expressing full-length consensus-sequence M2 (M2-DNA) induced M2-specific antibody responses and protected against challenge with lethal influenza. Mice primed with M2-DNA and then boosted with recombinant adenovirus expressing M2 (M2-Ad) had enhanced antibody responses that cross-reacted with human and avian M2 sequences and induced T-cell responses. This M2 prime-boost vaccination conferred broad protection against challenge with lethal influenza A, including an H5N1 strain. Vaccination with M2, with key sequences represented, may provide broad protection against influenza A.
topic DNA vaccines
M2 protein
influenza A virus H1N1 subtype
influenza A virus H3N2 subtype
influenza A virus H5N1 subtype
research
url https://wwwnc.cdc.gov/eid/article/13/3/06-1125_article
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