Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1
Changes in influenza viruses require regular reformulation of strain-specific influenza vaccines. Vaccines based on conserved antigens provide broader protection. Influenza matrix protein 2 (M2) is highly conserved across influenza A subtypes. To evaluate its efficacy as a vaccine candidate, we vacc...
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doaj-fee9914cef934acbac16c51803f85cd72020-11-25T01:04:37ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592007-03-0113342642610.3201/eid1303.061125Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1Stephen Mark TompkinsZi-Shan ZhaoChia-Yun LoJulia A. MisplonTeresa LiuZhiping YeRobert J. HoganZhengqi WuKimberly A. BentonTerrence M. TumpeySuzanne L. EpsteinChanges in influenza viruses require regular reformulation of strain-specific influenza vaccines. Vaccines based on conserved antigens provide broader protection. Influenza matrix protein 2 (M2) is highly conserved across influenza A subtypes. To evaluate its efficacy as a vaccine candidate, we vaccinated mice with M2 peptide of a widely shared consensus sequence. This vaccination induced antibodies that cross-reacted with divergent M2 peptide from an H5N1 subtype. A DNA vaccine expressing full-length consensus-sequence M2 (M2-DNA) induced M2-specific antibody responses and protected against challenge with lethal influenza. Mice primed with M2-DNA and then boosted with recombinant adenovirus expressing M2 (M2-Ad) had enhanced antibody responses that cross-reacted with human and avian M2 sequences and induced T-cell responses. This M2 prime-boost vaccination conferred broad protection against challenge with lethal influenza A, including an H5N1 strain. Vaccination with M2, with key sequences represented, may provide broad protection against influenza A.https://wwwnc.cdc.gov/eid/article/13/3/06-1125_articleDNA vaccinesM2 proteininfluenza A virus H1N1 subtypeinfluenza A virus H3N2 subtypeinfluenza A virus H5N1 subtyperesearch |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stephen Mark Tompkins Zi-Shan Zhao Chia-Yun Lo Julia A. Misplon Teresa Liu Zhiping Ye Robert J. Hogan Zhengqi Wu Kimberly A. Benton Terrence M. Tumpey Suzanne L. Epstein |
spellingShingle |
Stephen Mark Tompkins Zi-Shan Zhao Chia-Yun Lo Julia A. Misplon Teresa Liu Zhiping Ye Robert J. Hogan Zhengqi Wu Kimberly A. Benton Terrence M. Tumpey Suzanne L. Epstein Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1 Emerging Infectious Diseases DNA vaccines M2 protein influenza A virus H1N1 subtype influenza A virus H3N2 subtype influenza A virus H5N1 subtype research |
author_facet |
Stephen Mark Tompkins Zi-Shan Zhao Chia-Yun Lo Julia A. Misplon Teresa Liu Zhiping Ye Robert J. Hogan Zhengqi Wu Kimberly A. Benton Terrence M. Tumpey Suzanne L. Epstein |
author_sort |
Stephen Mark Tompkins |
title |
Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1 |
title_short |
Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1 |
title_full |
Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1 |
title_fullStr |
Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1 |
title_full_unstemmed |
Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1 |
title_sort |
matrix protein 2 vaccination and protection against influenza viruses, including subtype h5n1 |
publisher |
Centers for Disease Control and Prevention |
series |
Emerging Infectious Diseases |
issn |
1080-6040 1080-6059 |
publishDate |
2007-03-01 |
description |
Changes in influenza viruses require regular reformulation of strain-specific influenza vaccines. Vaccines based on conserved antigens provide broader protection. Influenza matrix protein 2 (M2) is highly conserved across influenza A subtypes. To evaluate its efficacy as a vaccine candidate, we vaccinated mice with M2 peptide of a widely shared consensus sequence. This vaccination induced antibodies that cross-reacted with divergent M2 peptide from an H5N1 subtype. A DNA vaccine expressing full-length consensus-sequence M2 (M2-DNA) induced M2-specific antibody responses and protected against challenge with lethal influenza. Mice primed with M2-DNA and then boosted with recombinant adenovirus expressing M2 (M2-Ad) had enhanced antibody responses that cross-reacted with human and avian M2 sequences and induced T-cell responses. This M2 prime-boost vaccination conferred broad protection against challenge with lethal influenza A, including an H5N1 strain. Vaccination with M2, with key sequences represented, may provide broad protection against influenza A. |
topic |
DNA vaccines M2 protein influenza A virus H1N1 subtype influenza A virus H3N2 subtype influenza A virus H5N1 subtype research |
url |
https://wwwnc.cdc.gov/eid/article/13/3/06-1125_article |
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