Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers

• Main Authors: Zhiqiang Mei, Boxu Zhou, Chunli Wei, Jingliang Cheng, Saber Imani, Hanchun Chen, Junjiang Fu
• Format: Article
• Language: English
• Published: MDPI AG 2015-11-01
• Series: Molecules
• Subjects: G. jasminoides Ellis var. grandiflora Nakai; Gardenia jasminoides; random amplified polymorphic DNA; sequence-characterized amplified region; cloning; genetic authentication
• Online Access: http://www.mdpi.com/1420-3049/20/11/19687

The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random amplified polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplified region (SCAR) markers. These markers have been deposited in GenBank database with the accession numbers KP641310, KP641311, KP641312 and KP641313 respectively. The BLAST search of database confirmed the novelty of these markers. The four SCAR markers, namely ZZH11, ZZH31, ZZH41 and ZZH51 can specifically recognize the genetic materials of G. jasminoides from other plant species. Moreover, SCAR marker ZZH31 can be used to distinguish G. jasminoides Ellis var. grandiflora Nakai from other G. jasminoides on the market. Together, this study has developed four stably molecular SCAR markers by improved RAPD-derived DNA markers for the genetic identification and authentication, and for ecological conservation of medicinal and ornamental plant G. jasminoides.