Numb regulates cell tension required for mammary duct elongation
The mammary gland undergoes extensive expansion of a ductal network through the stroma during puberty and is an excellent model for understanding epithelial tube morphogenesis. To investigate a role for Numb, a multifaceted adapter protein, in epithelial tube morphogenesis, we conditionally deleted...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
The Company of Biologists
2019-05-01
|
Series: | Biology Open |
Subjects: | |
Online Access: | http://bio.biologists.org/content/8/5/bio042341 |
id |
doaj-ff105bf5d49c4d86925fd435daf9be63 |
---|---|
record_format |
Article |
spelling |
doaj-ff105bf5d49c4d86925fd435daf9be632021-06-02T13:11:22ZengThe Company of BiologistsBiology Open2046-63902019-05-018510.1242/bio.042341042341Numb regulates cell tension required for mammary duct elongationSudipa June Chatterjee0Ruba Halaoui1Rebecca Catherine Deagle2Carlis Rejon3Luke McCaffrey4 Rosalind and Morris Goodman Cancer Research Centre, McGill University, Montreal, QC H3A 1A3, Canada Rosalind and Morris Goodman Cancer Research Centre, McGill University, Montreal, QC H3A 1A3, Canada Rosalind and Morris Goodman Cancer Research Centre, McGill University, Montreal, QC H3A 1A3, Canada Rosalind and Morris Goodman Cancer Research Centre, McGill University, Montreal, QC H3A 1A3, Canada Rosalind and Morris Goodman Cancer Research Centre, McGill University, Montreal, QC H3A 1A3, Canada The mammary gland undergoes extensive expansion of a ductal network through the stroma during puberty and is an excellent model for understanding epithelial tube morphogenesis. To investigate a role for Numb, a multifaceted adapter protein, in epithelial tube morphogenesis, we conditionally deleted it from the mammary epithelium. We report that Numb-depletion results in altered extracellular-matrix organization, reduced cell tension, altered cell shape, and increased cell packing density, which results in a 50% reduction in mammary duct elongation. Using laser ablation in vitro and geometric-based cell force inference in vivo, we determined that Numb-deficient cells have altered cortical tension. Duct elongation defects were associated with altered E-cadherin distribution, but were independent of proliferation, apoptosis in ducts or end buds. This highlights a critical role for Numb in a mechanical mechanism that is required to maintain cell packing density during epithelial tube elongation.http://bio.biologists.org/content/8/5/bio042341Mammary glandEpithelialMorphogenesisTensionE-cadherin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sudipa June Chatterjee Ruba Halaoui Rebecca Catherine Deagle Carlis Rejon Luke McCaffrey |
spellingShingle |
Sudipa June Chatterjee Ruba Halaoui Rebecca Catherine Deagle Carlis Rejon Luke McCaffrey Numb regulates cell tension required for mammary duct elongation Biology Open Mammary gland Epithelial Morphogenesis Tension E-cadherin |
author_facet |
Sudipa June Chatterjee Ruba Halaoui Rebecca Catherine Deagle Carlis Rejon Luke McCaffrey |
author_sort |
Sudipa June Chatterjee |
title |
Numb regulates cell tension required for mammary duct elongation |
title_short |
Numb regulates cell tension required for mammary duct elongation |
title_full |
Numb regulates cell tension required for mammary duct elongation |
title_fullStr |
Numb regulates cell tension required for mammary duct elongation |
title_full_unstemmed |
Numb regulates cell tension required for mammary duct elongation |
title_sort |
numb regulates cell tension required for mammary duct elongation |
publisher |
The Company of Biologists |
series |
Biology Open |
issn |
2046-6390 |
publishDate |
2019-05-01 |
description |
The mammary gland undergoes extensive expansion of a ductal network through the stroma during puberty and is an excellent model for understanding epithelial tube morphogenesis. To investigate a role for Numb, a multifaceted adapter protein, in epithelial tube morphogenesis, we conditionally deleted it from the mammary epithelium. We report that Numb-depletion results in altered extracellular-matrix organization, reduced cell tension, altered cell shape, and increased cell packing density, which results in a 50% reduction in mammary duct elongation. Using laser ablation in vitro and geometric-based cell force inference in vivo, we determined that Numb-deficient cells have altered cortical tension. Duct elongation defects were associated with altered E-cadherin distribution, but were independent of proliferation, apoptosis in ducts or end buds. This highlights a critical role for Numb in a mechanical mechanism that is required to maintain cell packing density during epithelial tube elongation. |
topic |
Mammary gland Epithelial Morphogenesis Tension E-cadherin |
url |
http://bio.biologists.org/content/8/5/bio042341 |
work_keys_str_mv |
AT sudipajunechatterjee numbregulatescelltensionrequiredformammaryductelongation AT rubahalaoui numbregulatescelltensionrequiredformammaryductelongation AT rebeccacatherinedeagle numbregulatescelltensionrequiredformammaryductelongation AT carlisrejon numbregulatescelltensionrequiredformammaryductelongation AT lukemccaffrey numbregulatescelltensionrequiredformammaryductelongation |
_version_ |
1721404255981535232 |