Spectroscopic quantification of soil phosphorus forms by 31p-nmr after nine years of organic or mineral fertilization

• Main Authors: Luciano Colpo Gatiboni, Gustavo Brunetto, Danilo dos Santos Rheinheimer, João Kaminski, Carla Maria Pandolfo, Milton Veiga, Alex Fabiani Claro Flores, Maria Angélica Silveira Lima, Eduardo Girotto, André Carlos Cruz Copetti
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Ciência do Solo 2013-06-01
• Series: Revista Brasileira de Ciência do Solo
• Subjects: glicerofosfato; estercos; fitato; fósforo orgânico; fertilizantes orgânicos
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832013000300010&lng=en&tlng=en

Long-standing applications of mineral fertilizers or types of organic wastes such as manure can cause phosphorus (P) accumulation and changes in the accumulated P forms in the soil. The objective of this research was to evaluate the forms of P accumulated in soils treated with mineral fertilizer or different types of manure in a long-term experiment. Soil was sampled from the 0-5 cm layer of plots fertilized with five different nutrient sources for nine years: 1) control without fertilizer; 2) mineral fertilizer at recommended rates for local conditions; 3) 5 t ha-1 year-1 of moist poultry litter; 4) 60 m³ ha-1 year-1 of liquid cattle manure and 5) 40 m³ ha-1 year-1 of liquid swine manure. The 31P-NMR spectra of soil extracts detected the following P compounds: orthophosphate, pyrophosphate, inositol phosphate, glycerophosphate, and DNA. The use of organic or mineral fertilizer over nine years did not change the soil P forms but influenced their concentration. Fertilization with mineral or organic fertilizers stimulated P accumulation in inorganic forms. Highest inositol phosphate levels were observed after fertilization with any kind of manure and highest organic P concentration in glycerophosphate form in after mineral or no fertilization.