Sperm morphology in Estonian and Tori Breed Stallions
<p/> <p>The standard procedure for assessing the breeding potential of a stallion includes the parameter total number of spermatozoa classified as morphologically normal. This study investigated sperm morphology of fresh semen in randomly chosen Estonian (E, n = 8) and Tori (T, n = 7) br...
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doaj-ff1a4ee7d6e94ff8a86c08940151239a2020-11-24T21:37:57ZengBMCActa Veterinaria Scandinavica1751-01472004-03-01451111810.1186/1751-0147-45-11Sperm morphology in Estonian and Tori Breed StallionsAidnik MLundeheim NKavak AEinarsson S<p/> <p>The standard procedure for assessing the breeding potential of a stallion includes the parameter total number of spermatozoa classified as morphologically normal. This study investigated sperm morphology of fresh semen in randomly chosen Estonian (E, n = 8) and Tori (T, n = 7) breed stallions with proven fertility. Two ejaculates were examined from each stallion. An aliquot from each ejaculate was fixed in 1 mL formol-saline immediately after collection and examined with phase-contrast microscope at a magnification 1000× for all types of morphological abnormalities. Furthermore smears were prepared and stained according to Williams (carbolfuchsin-eosin) for a more detailed examination of the sperm heads with light microscope at a magnification 1000×. Analysis of variance was applied to the data, and results are presented as LSmeans (± SE). One T stallion that had a disturbance in the spermatogenesis and one 22-year-old E stallion were not included in the analyses. The T stallions had on average 57.5 ± 4.1% and the E-stallions 74.4 ± 3.8% morphologically normal spermatozoa (p = 0.012). In 4 of 7 T stallions and 7 of 8 E stallions both ejaculates had >50% morphologically normal spermatozoa. There was a significant difference between breeds in mean percentage of proximal droplets (17.3 ± 2.7% and 2.9 ± 2.5% for T and E stallions, respectively; p = 0.003).</p> http://www.actavetscand.com/content/45/1/11semensperm morphologyhorse |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aidnik M Lundeheim N Kavak A Einarsson S |
spellingShingle |
Aidnik M Lundeheim N Kavak A Einarsson S Sperm morphology in Estonian and Tori Breed Stallions Acta Veterinaria Scandinavica semen sperm morphology horse |
author_facet |
Aidnik M Lundeheim N Kavak A Einarsson S |
author_sort |
Aidnik M |
title |
Sperm morphology in Estonian and Tori Breed Stallions |
title_short |
Sperm morphology in Estonian and Tori Breed Stallions |
title_full |
Sperm morphology in Estonian and Tori Breed Stallions |
title_fullStr |
Sperm morphology in Estonian and Tori Breed Stallions |
title_full_unstemmed |
Sperm morphology in Estonian and Tori Breed Stallions |
title_sort |
sperm morphology in estonian and tori breed stallions |
publisher |
BMC |
series |
Acta Veterinaria Scandinavica |
issn |
1751-0147 |
publishDate |
2004-03-01 |
description |
<p/> <p>The standard procedure for assessing the breeding potential of a stallion includes the parameter total number of spermatozoa classified as morphologically normal. This study investigated sperm morphology of fresh semen in randomly chosen Estonian (E, n = 8) and Tori (T, n = 7) breed stallions with proven fertility. Two ejaculates were examined from each stallion. An aliquot from each ejaculate was fixed in 1 mL formol-saline immediately after collection and examined with phase-contrast microscope at a magnification 1000× for all types of morphological abnormalities. Furthermore smears were prepared and stained according to Williams (carbolfuchsin-eosin) for a more detailed examination of the sperm heads with light microscope at a magnification 1000×. Analysis of variance was applied to the data, and results are presented as LSmeans (± SE). One T stallion that had a disturbance in the spermatogenesis and one 22-year-old E stallion were not included in the analyses. The T stallions had on average 57.5 ± 4.1% and the E-stallions 74.4 ± 3.8% morphologically normal spermatozoa (p = 0.012). In 4 of 7 T stallions and 7 of 8 E stallions both ejaculates had >50% morphologically normal spermatozoa. There was a significant difference between breeds in mean percentage of proximal droplets (17.3 ± 2.7% and 2.9 ± 2.5% for T and E stallions, respectively; p = 0.003).</p> |
topic |
semen sperm morphology horse |
url |
http://www.actavetscand.com/content/45/1/11 |
work_keys_str_mv |
AT aidnikm spermmorphologyinestonianandtoribreedstallions AT lundeheimn spermmorphologyinestonianandtoribreedstallions AT kavaka spermmorphologyinestonianandtoribreedstallions AT einarssons spermmorphologyinestonianandtoribreedstallions |
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